Perforin (PRF1) Polyclonal Antibody [Clone ID: dG9]

Specifications

Product Data
Clone Name	dG9
Applications	FC
Recommend Dilution	Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Reactivity	Human, Cow
Clonality	Polyclonal
Immunogen	purified granules from human YT lymphoma cell line
Specificity	The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Formulation	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Format: APC Label: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Concentration	lot specific
Purification	*Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134(3):326-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/21978002,*Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2(3):139-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/20348186 ,*Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8(11):e1002984., URL: http://www.ncbi.nlm.nih.gov/pubmed/23144609 ,*Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73(11):1050-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18615597 ,*Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1374586
Conjugation	Unconjugated
Storage Condition	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Gene Name	perforin 1
Database Link	Entrez Gene 5551 Human UniProt ID P14222
Synonyms	PRF1, P1, PFP, HPLH2
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.