FOLR1 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:1,000 ELISA: 1:1,000 - 1:2,500
Host	Goat
Clonality	Polyclonal
Immunogen	Folate Binding Protein [Bovine Milk]
Specificity	Anti-Folate Binding Protein is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk].  Cross reactivity against Folate Binding Protein from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P02702
Background	Anti-Folate Binding Protein antibody belongs to the folate receptor family and binds to folate and reduced folic acid derivatives. It further regulates delivery of 5-methyltetrahydrofolate to the cell interior. Folate expresses solely in epithelial tissues, and expression is increased in malignant tissues. Anti-Folate Binding Protein antibody is ideal for researchers investigating Tags & Cell Markers and Cancer research.
Synonyms	goat anti-Folate Binding Protein Antibody biotin Conjugation, biotin Conjugated goat anti-Folate Binding Protein Antibody, Folate receptor alpha, FR-alpha, Folate receptor 1, Milk folate-binding protein, Folate-binding protein 1, FBP
Note	Anti-Folate Binding Protein has been tested by western blot and is suitable to be assayed against 1.0 ug of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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