MSH2 Rabbit Monoclonal Antibody [Clone ID: 1B11-14-16]

Specifications

Product Data
Clone Name	1B11-14-16
Applications	ELISA, IHC, WB
Reactivity	Human
Modifications	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing BALB/c mice with human MSH2 protein peptide (KLH-NSFVNEIISRIKVTT-Cys) conjugated with KLH in equal volume of Freund's complete adjuvant.
Specificity	This antibody binds an epitope on the human DNA mismatch protein MSH2 between amino acids 919-934 (NSFVNEIISRIKVTT). MSH2 forms a heterodimer with MSH6 to make the human MutSα mismatch repair complex. The DNA mismatch repair system (MMR) is composed of enzyme molecules that specifically repair DNA base mismatches. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. Studies have shown that mutations and/or loss of function of MMR genes are related to the occurrence of a variety of tumors, such as colorectal cancer, skin cancer, ovarian cancer, and endometrial cancer. The binding specificity of this antibody for human MSH2 was determined using ELISA. This antibody was capable of detecting MSH2 from HCT8 and Biu-87 cell lysates in a western blot. This antibody was also used in the immunohistochemical analysis of normal tissues like tonsil, appendix tissues and cancerous tissues like colorectal and bladder cancer. This antibody shows similar staining of MSH2 protein compared to the commercially available antibody G219-1129 (CN110218251).
Isotype	IgG, kappa
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Database Link	UniProt ID P43246
Synonyms	CGMCC NO.17405; DNA mismatch repair protein Msh2; G219-1129; hMSH2; MutS protein homolog 2
Reference Data

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