NSE (ENO2) Mouse Monoclonal Antibody [Clone ID: UMAB287]

Specifications

Product Data
Clone Name	UMAB287
Applications	IHC, WB
Recommend Dilution	WB 1:1000, IHC 1:15000
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 188-293 of human NSE (NP_001966) produced in E.coli.
Isotype	IgG2b
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Predicted Protein Size	47.3 kDa
Gene Name	enolase 2
Database Link	NP_001966 Entrez Gene 13807 MouseEntrez Gene 24334 Rat UniProt ID P09104
Background	This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Synonyms	HEL-S-279; NSE
Reference Data
Protein Pathways	Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation

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