Mouse IgG3 (subclass specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, IP
Recommend Dilution	Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG3 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions 1/10 - 1/40, depending on the method used.
Reactivity	Mouse
Host	Goat
Immunogen	Pools of purified homogenous IgG3 isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: TRITC State: Lyphilized purified IgG fraction Label: Tetramethylrhodamine Isothiocyanate Absorption emission: 554 nm/573 nm Molar radio: TRITC/IgG: ~1,7
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	TRITC
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Fluorescent marker: Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell-counting procedures.

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