Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
Applications	IF
Recommend Dilution	Immunocytochemistry (1/200). In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivity	Chicken
Host	Goat
Immunogen	Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Formulation	PBS, pH 7.2 containing 0.2% BSA as stabilizer and 0.02% Sodium Azide as preservative Label: FITC State: Liquid purified IgY fraction Label: Fluorescein Isothiocyanate Molar radio: F/P = 3.2
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C in the dark.

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