Goat IgG (H+L chain) Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Goat IgG (H+L chain) Rabbit Polyclonal Antibody
Applications	ELISA, IF
Recommend Dilution	Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000.
Reactivity	Goat
Host	Rabbit
Immunogen	Goat IgG whole molecule
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: FITC State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Sodium Azide Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 4.2 moles FITC per mole of IgG
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation	FITC
Storage Condition	Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.