Human PBEF/ Visfatin ELISA Kit (48-well)

Specifications

Product Data
Description	Human PBEF/ Visfatin ELISA Kit (48-well)
Size	1 x 48 wells
Format	48-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	1.56-100pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human PBEF/ Visfatin Ligand
Sensitivity	0.70ng/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human PBEF/Visfatin (8X6) 2 vials human PBEF/Visfatin Standard lyophilized, 50 ng/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human PBEF/Visfatin antibody 2 vials Streptavidin-HRP solution 4 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	PBEF (preB cell colonyenhancing factor. also visfatin and nicotinamide phosphoribosyltransferase) is a 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the Cterminal 128 amino acids (aa 364491). Over aa 27491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.
Gene Symbol	NAMPT
Standard Curve	Representative standard curve for PBEF/Visfatin ELISA. PBEF/Visfatin was diluted in serial two-fold steps in Sample Diluent.

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