Human BDNF ELISA Kit

CAT#: EA100205

Human BDNF ELISA Kit



CNY 5,000.00


货期*
3周

规格
    • 1 x 96 wells

Cited in 1 publication.

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Specifications

Product Data
Description Human BDNF ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Human BDNF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human BDNF
Sensitivity <2pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-human BDNF antibody | 1
  • Lyophilized recombinant human BDNF standard | 10ng/tube x 2
  • Biotinylated anti-human BDNF antibody | 100ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20 ml
Background Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
Gene Symbol BDNF
Standard Curve
Representative standard curve for Human BDNF PicoKine ELISA Kit
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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