Mouse CCL7/MCP3 ELISA Kit

Specifications

Product Data
Description	Mouse CCL7/MCP3 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse CCL7/MCP3
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	15.6pg/ml-1000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of mouse CCL7
Sensitivity	<1pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- mouse CCL7 antibody 1 Lyophilized recombinant mouse CCL7 standard 10ng/tubex2 Biotinylated anti- mouse CCL7 antibody 100ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 100ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Wash Buffer (25x) 20ml Plate sealers 4x
Background	Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
Gene Symbol	Ccl7
Locus ID	20306

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