Human Seprase/FAP ELISA Kit

Specifications

Product Data
Description	Human Seprase/FAP ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human Seprase/FAP
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	62.5pg/ml-4000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human Seprase
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human Seprase antibody | 1 Lyophilized recombinant human Seprase standard: 10ng/tube | 2 Biotinylated anti- human Seprase antibody: 100ul (dilution 1:100) | 1 Avidin-Biotin-Peroxidase Complex(ABC) 100ul (dilution 1:100) | 1 Sample diluent buffer 30 ml | 1 Antibody diluent buffer 12ml | 1 ABC diluent buffer 12ml | 1 TMB color developing agent 10ml | 1 TMB stop solution 10ml | 1 Plate Sealers | 4 Wash Buffer (25x) 20ml | 1
Background	FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.
Gene Symbol	FAP

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.