Human Androstenedione ELISA kit

Specifications

Product Data
Description	Human Androstenedione ELISA kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	0.12-10 ng/mL
Sample Type	Serum
Sample Volume	25 µl/well
Specificity	This kit is used for quantitative detection human Androstenedione
Sensitivity	0.12 ng/mL
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	1. Microwells coated with Goat anti-rabbit IgG: 12x8x1 2. Standard : 6 vials (ready to use): 0.5 ml 3. Enzyme Conjugate (ready to use): 7 ml 4. Rabbit Anti- Androstenedione Reagent (ready to use): 7 ml 5. TMB substrate (ready to use): 12 ml 6. Stop solution (ready to use): 12 ml 7. Wash Solution 20x Concentrated: 25ml
Background	Androstenedione is the primary precursor of testosterone in women. It is synthesized in the adrenal gland. Measurement of Androstenedione may be used as an indicator of androgenic activity in women. The steroid hormone Androstenedione is one of the main androgens, besides Testosterone and Dehydroepiandrosterone. In males, androgens are secreted primarily by the Leydig cells of the testes, to some degree also in the adrenal cortex. In females, the androgens are secreted mainly in the adrenal glands and in the ovary. Around 10% of the androgens are derived from peripheral conversion, mainly of DHEA. Androstenedione and Testosterone show high diurnal variability. The highest levels are measured in the morning. At the age of puberty serum androstenedione levels rise, after menopause they decline again. High androstenedione levels are measured during pregnancy. In women, high levels of androstenedione (47-100% above normal) are generally found in hirsutism, mostly in combination with other androgens as testosterone and DHEA-S. Androstenedione overproduction is due to ovarian dysfunction or maybe of adrenal origin. High circulating androstenedione levels are found in women with polycystic ovaries and 21-hydroxylase effect. Significant lower androstenedione levels are found in postmenopausal osteoporosis.

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