Cortisol ELISA Kit

Specifications

Product Data
Description	Cortisol ELISA Kit
Size	1 kit
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	0 - 800 ng/mL
Sample Type	Cell culture supernatant, serum, plasma
Sample Volume	20 ul
Specificity	Natural and recombinant human Cortisol
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	See the product information for more details
Background	The ORIGENE Cortisol ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Cortisol in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Cortisol in the donor sample. The microtiter wells are coated with a monoclonal antibody directed towards an antigenic site on the Cortisol molecule. Endogenous Cortisol of a donor sample competes with a Cortisol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of cortisol in the sample. Having added the substrate solution, the intensity of color developed is proportional to the concentration of cortisol in the specimen sample.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.