Human Uromucoid (UMOD) ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human Uromodulin in cell culture supernates, serum and plasma (heparin).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human UMOD
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	312pg/ml-20000pg/ml
Specificity	This kit is used for quantitative detection of Human UMOD
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- human Uromodulin antibody | 1 Lyophilized recombinant human Uromodulin standard | 10ng/tubex2 | 1 Biotinylated anti- human Uromodulin antibody 100 ul | 1 Avidin-Biotin-Peroxidase Complex(ABC) 100 ul | 1 Sample diluent buffer 30 ml | 1 Antibody diluent buffer 12 ml | 1 ABC diluent buffer 12 ml | 1 TMB color developing agent 10ml | 1 TMB stop solution 10 ml | 1 Wash Buffer (25x) 20 ml | 1 Plate Sealers | 4
Background	The Tamm–Horsfall glycoprotein (THP), also known as uromodulin, is a glycoprotein that in humans is encoded by the UMOD gene. The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants.
Gene Symbol	UMOD

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