Human Acid sphingomyelinase (SMPD1) ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of human SMPD1 in cell culture supernates, serum and plasma (heparin).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human SMPD1
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	15.6 pg/ml-10.000 pg/ml
Specificity	This kit is used for quantitative detection of Human SMPD1
Sensitivity	<10 pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti-human SMPD1 antibody | 1 Lyophilized recombinant human SMPD1 standard | 10 ng/tube x 2 Biotinylated anti-human SMPD1 antibody | 100 ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100) Sample diluent buffer | 30 ml Antibody diluent buffer | 12 ml ABC diluent buffer | 12 ml TMB color developing agent | 10 ml TMB stop solution | 10 ml Adhesive cover | 4 Wash Buffer (25x) | 20 ml
Background	Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), is an enzyme that in humans is encoded by the SMPD1 gene. This gene is mapped to 11p15.4. The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. Sphingomyelin phosphodiesterase 1 belongs to the sphingomyelin phosphodiesterase family.
Gene Symbol	SMPD1

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