Mouse Clec4f ELISA KIT (1 x 96 wells)

Specifications

Product Data
Description	For quantitative detection of mouse CLEC4F in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Sandwich ELISA kit of Quantitative Detection for Mouse CLEC4F
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	625pg/ml-40000pg/ml
Specificity	This kit is used for quantitative detection of Mouse CLEC4F
Sensitivity	<50pg/ml
Reactivity	Mouse
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Components	96-well plate precoated with anti- mouse CLEC4F antibody | 1 Lyophilized recombinant mouse CLEC4F standard | 40ng/tube×2 Biotinylated anti- mouse CLEC4F antibody | 130μl(dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Adhesive cover | 4
Background	C-type lectin domain family 4 member F is a protein that in humans is encoded by the CLEC4F gene. This gene is mapped to 6; 6 C3. CLEC4F is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain that contains an extended stalk region plus one carbohydrate recognition domain. Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation. CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues. CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner. Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system.
Gene Symbol	Clec4f

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