Human IL3 ELISA KIT (96 T)

Specifications

Product Data
Description	Human IL-3 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	15.62-1000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human IL-3.
Sensitivity	8 pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,1000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.
Gene Symbol	IL3
Locus ID	3562

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