TUT7 (NM_024617) Human 3' UTR Clone

Specifications

Product Data
Product Name	TUT7 (NM_024617) Human 3' UTR Clone
Vector	pMirTarget
Synonyms	PAPD6; TENT3B; ZCCHC6
ACCN	NM_024617
Insert Size	974 bp
Sequence Data	Insert Sequence (showhide) >SC211291 3’UTR clone of NM_024617 The sequence shown below is from the reference sequence of NM_024617. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC TCAGCGAAGAGGACCCAGCAGGAATCATGAGGGAAGGAAAATGCAGCACTCTAAATGGCCACTCAGGCG TTCCTATTCACTCGGAAAATTAGGTTCATTTCACAGGACACAGCAGTGTAGATCAGGCTTCAACTTAAC ATTTAAGGGAAATGTCAGATTTTTTTTTAATTTAATGAAATTGTTAATGAGGAAAAATTTTTAATATAG TCTTATCTACCACACATCCCCATAGATTTAAGGATTTTAATAGAAAGACATGATGTATGTATTTAAGCC ACGTTAAAAGAAAAAATATAACTATGGACCGGTATTCAGTGAATACAGTTTCATGGTTTTTAATTCTTT CAAAGCACATTAAAAATGGTGTGCTGATAAACCCCAAGTAAATTAACCCTTTTTCCGTATAAATCCATT TTTTGTTTTGAAGAGGGGAAATTATATTTATTGTTGTTTACTGAATCCTGGTGTGAAAGCATATCAGAT ATGTATGAACTGCTACTGCTGTACTTCCGATTTACGGACATCATTTTATTGCTATTTGTAGACGTGATA ACATGAACATGAGTACCTATTTATGTGGGCCTTCAGTGGATGGGCAGTGCCACTCAGGTCTCTGGGGTT TCCCTCTCTAATTTTAAGTAAATTGACATATAACTACTATGCTTATAAAAATGAAGTAAGGAAAACAAG TAGTCCTGTTTGCCACTAAAAACATTTTCAAAGGAAAAATAAAATGAAAGTACTTTTTACTTTTTATGA TACTCAGAAATTAGGATGAAGAACTTTTAAAATTGCTGAAGATCAAAGAGGTTATCTCTGCCAGTCACA AGTGTGGCTGGTGTCATTCTGGGTCTGACTGGAGCCCTCCTGGACTGTTTCTTTAATTTCAAAAGCCCT GCAGACATAGTACCTGGTCAGAACTATGCCTCGGTTTATTTATCATTTTGAAATAAAATCAGAATTTCA ACCTGTAA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites	SgfI-MluI
OTI Disclaimer	Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq	NM_024617.4
Synonyms	PAPD6; TENT3B; ZCCHC6
Summary	Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:25979828, PubMed:25480299, PubMed:19703396, PubMed:22898984, PubMed:18172165, PubMed:28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed:30122351).[UniProtKB/Swiss-Prot Function]
Locus ID	79670
MW	37.3

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