Ift20 (BC002040) Mouse Untagged Clone
CAT＃: MC200244
Ift20 (untagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790), (10ug)
CNY 1200.00
CNY 2000.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | RP23-399H5.8 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC002040
TGCTTCTGGACTCGGGGGTGACGTGTGGCTCCTAGATCTTGTCTCCGTGGCAGCAGCCGGTTGCAGTTGC CGGAGAGAAGCTTCTACCGACAGTCGTCAGGGCCGCAGAGCGCTGCCGAGGCGGCTCGTCAGCTAAGCTG TGCTGCTCCAGGGCAGGCACCATGTTGCATTCATCTTTACGCCCAGCATCTAGCAGTGTCGGCATGTAGT AGGCACTCAAGAAATGTATGTTGAATGAACGATGTCTGTGACAAGCAACTGGACTTTCCTCTTTCCTGAC CCTTGCTCCTATGACATACCTCCTGATTGCCACTGTCACCCCTTCAAAGCAGACCTCTAGGGCTTCCTGG AAGGGAAACAGCTATGGCCAAGGACATCTTGGGCGAGGCAGGGCTGCATTTTGATGAACTGAACAAGCTC CGAGTGTTGGACCCAGAGGTCACCCAGCAGACTGTGGAGCTCAAGGAGGAGTGCAAGGACTTTGTGGACA AAATTGGCCAGTTTCAGAAAATTGTTGGTGGTCTAATTGAGCTTGTTGATCAGCTTGCCAAAGAAGCAGA GAACGAGAAGATGAAGGCCATTGGTGCTCGGAACTTGCTGAAGTCCATAGCGAAGCAGAGAGAAGCCCAG CAGCAGCAGCTGCAGGCCCTGATAGCAGAAAAGAAGACGCAGCTAGAAAGGTAAGCGGCCATGGCCACTG CAAACGCCTTTTCCTTCTCTGCCACCTTGGCTTCCCTCACCATTTCCCAGGTAGAGCATCTCACTTTAAA AATTGATAGAAGAAAGTGGGGACTTGGTTAATTCCAAATTGCCTCCATGGTGTGAGCACTGGTTCTTATA ACAAGGACTGAGGCCACCTCCCGTGCCTTCCCTGGGGTAGAGTGTTCTCCCAGGTGGCCCTAAACAGGGC TTTGCTTATCTGACACTCTGGCCCTGCCTTCTCAATTCATGGTTTCTTGCATCTGTTTTAAAGCTCTATA CACTCAAAGAATTATGTTTTTATTTCATTCTTTTCAGGGCTTCTAACAATTTTGTTTTTCAGGTATCGGG TTGAATATGAAGCTTTGTGTAAAGTAGAAGCAGAACAAAATGAATTTATTGACCAATTTATTTTTCAGAA ATGAACTGAAAATTTCAGTTTTATAGCAGGAAGGAAAAAAAAAAAAAGCACCCCAACCAAAAAAAAAAAA AAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC002040 |
| Insert Size | 321 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC002040, AAH02040 |
| RefSeq Size | 1193 bp |
| RefSeq ORF | 321 bp |
| Locus ID | 55978 |
| Gene Summary | Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium (PubMed:16775004). Regulates the ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling (PubMed:29237719). Essential for male fertility. Plays an important role in spermatogenesis, particularly spermiogenesis, when germ cells form flagella. May play a role in the transport of flagellar proteins ODF2 and SPAG16 to build sperm flagella and in the removal of redundant sperm cytoplasm (PubMed:27682589). Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment (PubMed:24089209).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG200367 | Ift20 (tGFP-tagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790) |
CNY 2850.00 |
|
| MR200367 | Ift20 (Myc-DDK-tagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790) |
CNY 1200.00 |
|
| MR200367L3 | Lenti ORF clone of Ift20 (Myc-DDK-tagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790) |
CNY 4750.00 |
|
| MR200367L4 | Lenti ORF clone of Ift20 (mGFP-tagged) - Mouse intraflagellar transport 20 homolog (Chlamydomonas) (cDNA clone MGC:6005 IMAGE:3590790) |
CNY 4750.00 |
