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Nek2 (BC010302) Mouse Untagged Clone
CAT＃: MC200695
Nek2 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 2 (cDNA clone MGC:11846 IMAGE:3597227), (10ug)
CNY 2,000.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA617254; C77054 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC010302
CGGACGCGTGGGCGGACGGTGGGCGGTTGGCCTGCAGCGGGCGAGCCTGGGCTCCGAGCGGCGCGGTGGT GTGGTTGCCAGGGTGGCCGCTCGCCATGCCGTCCCGGGTGGAGGACTACGAGGTGCTGCACAGCATCGGC ACCGGCTCCTACGGCCGCTGTCAGAAGATTCGGAGGAAGAGCGACGGCAAGATCCTGGTGTGGAAAGAGC TTGACTATGGCTCCATGACGGAGGTGGAGAAGCAGATGCTTGTGTCTGAAGTGAACTTGCTTCGGGAGCT GAAACATCCAAACATCGTCCGTTACTATGATCGCATTATTGACCGAACCAACACAACCCTGTACATCGTA ATGGAATACTGTGAGGGAGGGGACCTGGCTAGTGTCATTTCAAAGGGGACCAAGGATAGACAGTACTTGG AAGAAGAGTTTGTCCTTCGAGTGATGACTCAGTTGACGCTGGCCCTGAAAGAGTGTCACAGAAGGAGCGA TGGTGGCCACACTGTGCTTCACCGGGACCTGAAGCCAGCCAATGTCTTCCTGGACAGCAAACACAATGTC AAGCTGGGGGACTTTGGACTAGCTAGAATATTAAATCACGACACGAGTTTTGCAAAAACGTTTGTTGGCA CACCCTATTACATGTCTCCTGAACAGATGAGCTGCTTATCCTACAACGAGAAGTCGGACATCTGGTCCTT GGGCTGCCTGCTGTATGAGCTGTGTGCACTAATGCCTCCCTTTACAGCTTTCAACCAAAAAGAGCTAGCT GGGAAAATCAGGGAAGGGAGGTTCAGGCGCATCCCCTACCGCTACTCTGATGGCTTGAATGACCTCATCA CTCGGATGCTGAATTTAAAGGACTACCATCGACCTTCAGTGGAAGAAATTCTGGAGAGCCCTTTGATAGC AGACATGGTTGCAGAAGAGCAAAGGAGAAATCTGGAGAGGAGAGGACGGCGCTCAGGCGAGCCTTCGAAG CTGCCGGACTCCAGCCCTGTGCTGAGCGAGCTCAAGTTGAAGGAAAGGCAACTGCAGGATCGAGAGCAAG CACTCAGAGCTCGGGAGGACATTCTGGAGCAGAAGGAACGTGAACTTTGTATTCGAGAGAGACTTGCAGA GGACAAACTGGCCAGAGCCGAGAGCCTGATGAAGAACTACAGCCTGCTGAAGGAGCACAGGCTCCTATGT CTGGCTGGTGGCCCAGAACTTGATCTTCCATCCTCAGCCATGAAGAAGAAGGTTCATTTCCACGGGGAAA GCAAAGAGAACACCGCAAGGAGTGAGAATTCTGAGAGCTACCTTGCCAAGTCCAAGTGCAGGGACCTGAA GAAGAGGCTTCATGCTGCCCAGCTGCGGGCTCAAGCCCTGGCTGATATTGAAAAAAACTACCAGCTAAAG AGCAGGCAGATCCTGGGCATGCGCTAGGCCGGCAAGGCATGGAGCTGGGTCAGTGTTGATACTGACAACC CACTAGAGATTGGTATTCAGCTGCTGTCGTTTTGTGTGTCTGGTTCTGTGGGCAGGAACCTTTGTGTGTG GTGAGCTCGTGGCATTGCTTGTGGTCTGCAAATGGATGTGTGTGTCTGCTTCCTAATGTCCCTGTGTGAA AAGCAAGCTGTCTTTGCTGGTTGGTTGGGCTTTTGATCCTGTGTGTGATTACTACTTGGAATATGAGATG GGGCACTATAGATACTATAGATCTTAGGAGAAAATAATGTTAGGGAAAGAATATTTAACCTGGAGGGCTC AGAGAGGCTCACACGCTGAGTGATACCGCCTTCACTGTGGTTTCCAAGTGAGGCCTCAGCGGGTCCATGA CCTCACTGCTAACGGGATGCGTATGGCAGGGCCCACAGGGTTGCATGTCAGAGGTGTTGTAATGTTACAG GGAGAGACCCAGCCTCTGGCCTGACCTCCTGATGAGAAGCCACTCACCAGCATTCCCATTCCAGGACGCT CACTGTGCCAGTTAACATCAGATTAGAAGTGGAGGGGAGGGGAGGGCGAGGGCTGAAGCTTTTAGGATTT GCCTTAGGGAAGCGCGTGCCATGGCCCTCAGCACGCTGCTGTCCTGCATCCTAGGCAGGCACTGGGGACA GGAGGCTCTGGGAGCCCTGAGAGCCCCCTCCTGCTGTGTGCAGGGCCTGTCCCCGTCTGTCTGTATCTGA CCTCTAGGTCAGTTGATGACATTAGTTAAGTGTTTCTGTACCTACAAGTATAAGCCAAAAGGTCGGAAAT GCCTCAGAGTCACACATGTGGTCCCCAAACATTTAATTTCTGAAGAGAACTGTCTTCAGAAAGTTGGTCT ATTTAAGGTGACTGGGGAAAAGGCAGGTTTCCTTTGCTCTCTGTCCACAGTCCCTTGAGATGGCTTCAGA AGAGAACAGTGTTCAGTGTGCGTTATCGAGGGCCCTGTATGCCTTCACCTGTGTGAGGGCCAGATTGCTG TTTGTCTACGGTTTCTTGAAGACCTCAGCTCAGCAGAAAGGAGTTCATTTGCCCTAACAAGTACTGTTGG CTGAGAGGGGAGGTCAGAAGCATGTAATGCCATTCGTTTCATGGCTAAACACACAGACTCCTTGCATTTT AAAATTCTGTGCCTTTACTTCCTGGAGCTATAATAACGTGCTTGGGTGTGTGACTTGCCGAGGTTGGCTT TCGCAATGCTTTATGTAAACTCTGCTGTTGGCACTTGCAGCTGTAGAAGCTGTCGGAGGGATTACTTACT TACGGTTATAGCATCTGGGGTCTCTTGCAGCTGCAATGCCTGGGATCTTTTGCAACTCTGACTTTTTCAG TAGCAGCTTGGAATGTTAAAATATGGTGAGCCTCTTCTCTGTGAAGGAAGGGCCTGACAATCTGCCAGCA GGGAAAAGACTGGGAACTCAGTGCAGTGGTTTTCCTGCCTTTCCCTTCTCCTTTGGTCTATAGTGATACT GACAGCAATACAAAGTAAAATCTGTGTATATAAAAATCCTAAGTATGAGGTCTTAAATGTTCTGGAATTT TAGAAAAAACTTAATTGGATGCAATTGTTTATAATTAACAAGTCATCTTATTTATAAATTTTCCAATATA ATATAAATCTATTTTGTGGAAATTTA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC010302 |
| Insert Size | 1332 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC010302, AAH10302 |
| RefSeq Size | 3106 bp |
| RefSeq ORF | 1332 bp |
| Locus ID | 18005 |
| Gene Summary | Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (By similarity). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (By similarity). Phosphorylates and activates NEK11 in G1/S-arrested cells. Involved in the regulation of centrosome disjunction (By similarity).[UniProtKB/Swiss-Prot Function] |
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