Eif5 (NM_173363) Mouse Untagged Clone
CAT＃: MC204145
Eif5 (untagged) - Mouse eukaryotic translation initiation factor 5 (Eif5), transcript variant 1, (10ug)
CNY 3656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2810011H21Rik; D12Ertd549e |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC039275
CGGCGTTGTTCAGTCAGAGCGAGAACATTCCAGAGGTCGCCAGGCTCGGCGCTGCTGGTGTGGACGCGGA CGTCGCTGGGACAGTCCCTCCCGCTGCTCGGCGGCGGCACCTGCCCAGCCGCCCCTCCGCTCGCCGCCGG CCCCGCCGCTCCGGACCCGGTTTCCAGCGCCTCTTCCTCCCAGTCCCGGGCGAGCCGCGTTGGGTTTATG TCTTTATTTGACGAAAACGAGCTGTTGCGCAGCCATTGGTACCTGTATTGGGGAAACATAGCATACAAGC AAGAAGCTTACAGCCTCAGTGGCGAAAATTTTTTCATGTCAGAGACCGAGAACTCTTGCAGTCGTTTATG TCATCCCTTCTTCTCCAGACAGAAGATACCAAAAAGTTGCAATCAAAGATCTCTTCATCTTATTGATAAA GTCACTAATAAGCCAAAATGTCTGTCAACGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGAT GCCCCGTTTGATTGCTAAGGTTGAGGGCAAAGGAAATGGAATCAAGACAGTTATAGTCAACATGGTTGAC GTTGCAAAGGCGCTTAATCGGCCTCCAACGTATCCCACCAAATATTTTGGTTGTGAGCTGGGAGCACAGA CCCAGTTTGATGTTAAGAATGACCGTTACATTGTCAATGGATCTCATGAGGCGAATAAGCTGCAAGACAT GTTGGATGGATTCATTAAAAAATTTGTTCTCTGTCCTGAGTGTGAGAATCCTGAAACAGATCTGCATGTC AATCCAAAGAAGCAAACAATAGGTAATTCTTGTAAAGCCTGTGGGTACCGAGGCATGCTTGACACACATC ATAAACTCTGTACATTCATTCTCAAAAACCCACCTGAGAATAGTGACATTGGTACAGGAAAGAAAGAAAA GGAAAAGAAAAATAGAAAGGGCAAGGACAAGGAAAATGGCTCTGTATCTACCAGTGAGACACCACCACCT CCACCACCAAATGAAATTAGTCCTCCACATGCTGTGGAAGAAGAGGAAGATGATGATTGGGGGGAGGATA CAACTGAGGAAGCTCAAAGGCGCAGAATGGATGAAATCAGTGACCATGCAAAAGGTCTGACACTTAGTGA TGATTTGGAAAGAACTGTAGAAGAGCGTGTTAACATCCTGTTTGATTTTGTTAAGAAAAAGAAAGAAGAG GGCATTATTGATTCATCTGATAAAGAAATTGTGGCTGAGGCAGAAAGACTGGATGTAAAAGCCATGGGTC CTCTTGTTTTGACAGAAGTTCTCTTTGATGAGAAGATAAGAGAGCAAATCAAGAAATACAGGCGCCATTT TCTAAGATTTTGTCATAACAACAAAAAGGCTCAGCGGTACCTTCTTCATGGTTTGGAATGTGTGGTAGCA ATGCATCAAGCTCAGCTGATCTCCAAGATCCCACACATCCTGAAGGAGATGTATGATGCCGACCTGTTAG AGGAGGAGGTCATTATCAGCTGGTCAGAAAAGGCCTCTAAGAAATATGTCTCAAAAGAACTTGCCAAAGA GATTCGTGTCAAAGCAGAGCCATTTATTAAATGGTTGAAGGAAGCAGAGGAAGAATCTTCTGGTGGTGAG GAAGAAGATGAAGACGAAAATATTGAGGTCGTATATTCGAAAACTGCCAGTGTACCAAAAGTTGAAACTG TGAAGTCTGACAACAAGGATGATGACATTGATATTGACGCCATTTAAAAGGATGGATGCAACTTAGCTTA ACAGTGTAATGCTGCAAATTTTTCTCCATTATCAGCCAGAAGTGCAACATGTATGTGCAAGAGCTAAAGT GGCTTAACATCATGCTACACTTGATACTAAAAAGCTATTACTGTGAGTGGTCTATAATTAAGCCCAATGA GACATCTAGGGAGTCCATACATATCAGTGAGCAGTTGTAGTTTGCTTATTTATAGCATGTTTCTTTCGGA AAAACCTAGTGGTGGACACATTTGGATCACATTTATACAGTTATAAAAAATAAAGATTTGATTTTGGTCA TTCTTCAGACTTTGGGCTATGAATGGCTTATACTGAAGTAATTGGCTACTTTTAGGATGTTACACCATTT ATTTTAGGCTCCTTGAGTTTGATGGGTTGTTAGGAACTGAAAACTTGAAGAATGCAAACAAATATAATGA ACTTACTTAGCCATTAAGAAATGAAGTATTTTGAAAGTTGTATCTCCAGTCCCAGTCCATTCAGATTGGC AACTGACAATTCTTGTCATTCTAAGGAAATTTGGTGATTAAATGACAGTGTGACATCCTCGTAAGAAGTA AAAATGACCTGTGTGTCCTATGGTTTAAGAGCAAATTTTAAAACTTGGAAGTTGTGGTTTTCCAGTTTGT GTACACTCACCCCCAAATTGTAGTCTTTCAAGTCGTGTGCATTGCACGTTGGATAAGCCAGGGAAATTGT TAACAAATAAGTATTTTGTGTGTGTTTAGTGGTTGCTTTGTATTGAGAGAAAAGCTTTGAGGTGTGATTA AATCGTAAGCTCTGATTCTATTTGGGAGAAACAGGAAAAAAGGTGCACTTAATCTCTAGCTAAAACAGCG TAATTTTTCAACTTTTACCCTTAAATTATAATTTCAAGATGTTTAGACATACTGTATCTTGTGTTTGTGT ACCCCCTCCCCAATATTATGGTTTATTCTTTAATGCCTTTTAATTTGGATATAATAGCTTGTACTTTAGA TTTTGGTTGCTATCTTGCCAAAATAAGTGTTACTGTTTTTCAAGCTTGACCCCCTTCCCCAATTGTCTTA TTTAAAGAGAAAGTTAAACTCATACTTCTGAGTCAGAGCCTGTATTTTGGTGTAAGACTTGGGATATTTT TTACTTCACATTGAATATAGCTGGATACCTGAGAGACTGATGGCCACTGGGTGGGTGCAGCTAGCTAAGG CCTGACCAGCCCATTCAGAGCCTTGGACTTCAGACACAAAAGTGAGTTCTTACACACTTGCTGGTGTAAG CTCTATCTGGGGTCCTGACTATATTTGAAAACTTGTCTTCAATATTAAAAACAGCACATATTTCTTTCTA CAAAAGTGCATTCTGGGAGTTAAAAATCCATGTGGTTAATTTGTGTGTGGCGTGCTAGCTCATACATTAT TTGAATTTCATTCTTTGTGTCATCCATCTCACAGATTATTAGACTTTAATAAACAATGTAAAAATGTGCA TTAAAATCATACAAATATTTAGTAAAATTAGAACTTTAATAAGAGTTTGAGTGTGGAGCAGGAGCCATAC ACCTGGAATGGTAACAAGGGAATGTGCTGTGTCACACCAAAGATGTGGTACTTGGAAAGTCACTTGTCTC CTGGGTTTCAGATTCTTTGTTGCATGGCCCGCCCATCTCCATTTGTCATCATTTTTTGAGATTCTCAAGT ACATCAGCACATTCGGCCTCAGGTTGGCAAGATTTTTGTCTTAGAGCTGTTGCTTTAAAGGGAAACGGTC AGGTCTTAGACACTTAGGAAGGTCTTGGGCTTCTGTTCATTCTGGTGCCAAACCAGTGGGATTCAAATTT CACACAATCTGGGTTTTACTCATGGAGGTTAACCTGATAGGAGTAATCCTTCGTTGCTCTATTGAGGTGT CATAAAGTTTCCTGTTTCAAACAGCTACATTACTTGATTAAAACAATGTTAAAATTAAAAAAAAAAAAAA A |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_173363 |
| Insert Size | 1290 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC039275, AAH39275 |
| RefSeq Size | 3711 bp |
| RefSeq ORF | 1290 bp |
| Locus ID | 217869 |
| UniProt ID | P59325 |
| Gene Summary | Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represent the longer transcript. Variants 1 and 2 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206835 | Eif5 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 5 (Eif5), transcript variant 1 |
CNY 3656.00 |
|
| MR206835L3 | Lenti ORF clone of Eif5 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 5 (Eif5), transcript variant 1 |
CNY 5230.00 |
|
| MR206835L4 | Lenti ORF clone of Eif5 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 5 (Eif5), transcript variant 1 |
CNY 5230.00 |
