Timm22 (BC055279) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Tim22
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC055279 TGAAAGGTACTAGAGCCCCTACGGTCATGGCGGCTACCGCCCCCAAGGCCGGGGGTTCAGCTCCTGAGGC AGCGGGTTCTGCCGAAGCTCCGTTGCAGTACAGCCTTCTTCTTCAGTACCTGGTGGGCGACAAGCGTCAG CCCCGGCTTCTGGAGCCTGGCAGCCTGGGCGGGATCCCGAGTCCTGCCAAGAGCGAGGAGCAGAAGATGA TCGAGAGGGCAATGGAAAGTTGCGCCTTCAAGGCTGTGCTGGCCTGTGTAGGAGGGTTTGTCTTGGGAGG CGCGTTTGGTATCTTTACTGCTGGCATTGATACCAACGTAGGCTTTGACCCAAAGGACCCTTACCGGACA CCAACTGCAAAAGAAGTCCTGAAAGACATGGGACAGAGAGGAATGTCCTATGCCAAAAACTTTGCCATTG TGGGCGCCATGTTTTCATGTACTGAGTGTCTGGTAGAGTCTTACCGGGGAAAGTCGGACTGGAAGAACAG CGTCATCAGTGGCTGCATCACTGGCGGAGCCATCGGCTTCCGAGCTGGAGTAAAGGCCGGGGCCATAGGT TGTGGAGGGTTTGCTGCTTTCTCTGCTGCAATCGATTATTACCTACGGTGAAAGAAACAGCCGGGAAGGA AAGAGGACGCCAGCCACCTTCAGAGCTGCTCTGTGGAGCAGTTTCTACACACTTGGACTGCTTCAGGGGT GAAGCCAAGTGCATCCCTCTACAGTTGATGGTGTGAAGGACACACCTCGCTGCTCGATCGCTCTCTGCAG CCATTGGAACAACCATACTTTTCCACTTGAATTCCAACCAGGTTTTTCAGGGGAGGTGGTATGGCAGAGT GTCTCCTTGCAGTAAACAGATCAGGAGGCAGTGTGTGGACTGTTACGCATTAAGCTTTGAACCAGGCTCA GTTCTGACCTGGCTCAGCTCCCCAGCTTTGAATAGGGAAGTGGCAGTGTTTGCCCATAGCCGGGAAGCTC TTCTTCCTTCTGTGTGCAAGTATCCATCATGAGTCCCAGGGCAGGGCAAAGTAAACTTATGCCATCGAGG CAATTATGTGATTTCAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC055279
Insert Size	585 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC055279, AAH55279
RefSeq Size	1091 bp
RefSeq ORF	585 bp
Locus ID	56322
Gene Summary	Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity).[UniProtKB/Swiss-Prot Function]

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