Prnd (BC025140) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Dpl, PrPLP, doppel
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC025140 GCTTCAGAGGCCACAGTAGCAGAGAACCGAGATTCACCATGAAGAACCGGCTGGGTACATGGTGGGTGGC CATCCTCTGCATGCTGCTTGCCAGCCACCTCTCCACGGTCAAGGCAAGGGGCATAAAGCACAGGTTCAAG TGGAACCGGAAGGTCCTGCCCAGCAGCGGCGGCCAGATCACCGAAGCTCGGGTAGCTGAGAACCGCCCAG GAGCCTTCATCAAGCAAGGCCGGAAGCTGGACATCGACTTTGGAGCAGAGGGCAACAGGTACTACGCGGC TAACTATTGGCAGTTCCCTGATGGGATCTACTACGAAGGCTGCTCTGAAGCCAACGTGACCAAGGAGATG CTGGTGACCAGCTGCGTCAACGCCACCCAGGCGGCCAACCAGGCTGAGTTCTCCCGGGAGAAGCAGGATA GCAAGCTCCACCAGCGAGTCCTGTGGCGGCTGATCAAAGAGATCTGCTCCGCCAAGCACTGCGATTTCTG GCTGGAAAGGGGAGCTGCGCTTCGGGTCGCCGTGGACCAACCGGCGATGGTCTGCCTGCTGGGTTTCGTT TGGTTCATTGTGAAGTAAAAATCAATGAAGCTGGCAGCCACAGAGGACTCCAGGAGTTGCTGAGCGGGAT CTGGAAATAACTGCTACTGGGTGTAGTCTCTTGTAAACGGAGTATCTGCCAGGGGGGTCGGGTATTGTCA AAGGATTCATCTGCAACATTCCAGAACACGAGTGCTGATAGCAGTACTTCGAGTGCTGAGTTCCCAGTGC AATCGGTTCTTCTCAAGGACACCTCTGGCAGGTGTCACATGGTAAAGGGACATGAAGCAGGGCGGAACCT TTACCACTGGACAGACAGCCCAGAGAAACCAGCCTGCTGCAGTACGCCACTGCTGCCAAGACACACCTCC TCAGTCTACTGATCCTAAACCAGAGAGCAGGCCCAGATCACACAGTTTACCCCTTGCACGCCATTTTAAA TATCAGACAATAAAGAAGGAATAGTTAGTTCTTAGATGGTGATGTTGGTGTTTCAGCTCCAAGCTTAAGG CTTAGTTACCCGGGTGCACAAAGCTGTAGACAACCAACTTCCTTCCAGCGTGAAAATGTGTAGGCTGGAC AGAGTCCCGTGCTCCACGATCGCTGAGTTCTTTCAGACCATTGGTTGTCACACCCACAAGCCTAACTGTG ACAGCCAATGAGTGTCCATGTGATTTTGACTGCAAATTTCGCTTATTTGATTTTTAGCGGAGACGTCCTT GCTCTTACTTGTGGATCAGGAGAGGTTTTCTTAGCCCTTCTAAGTGTTCCCAACCCGTGCGCTGTAGAAC AGATTGCGCAAACTGACAGCAGAGTAAAAGGGGGCGGGGCTTCACTTCCGGCATCCCACCAGTCCACGCC CGCCGTTTTCACCTCTCCAGACTCGCCACTCCTTTATTCATTTGCAACTGCAAATGTAGCAATTTGCAAT CCTAATACAGGGCCAGGGCGGTGGATACCTTAAAGTTTTATTTGACTTCATTTAGTGCTTTCTGTCCATT TTTAATGACTTTTGTTTTTTTGTTTTTGTTTCTCCAGCCCCATCTCCTCCCTCTGCCCTAGTATGAAACT TGTACCCCTTTACTGTGTTTAAATATAAAGAAGAGGGGTTTTGTTTTGTTACATTACAACCCTTAAAATA CAACGATCACTTGTATAATATTTGTTTCTCTGCGGTTTGTATTCCTGATTTAGTGGAATGCCATTAAACT CTCTGGGTCAGTATCTAACTAGTTAACATCTAACTAGTCCTTTTAGATGACCATGAAGTATTCCACAGTA CAGATGTGCTGCAAGTGACTTGATAACTTCCTTGTTGACAGATCTTCAGATTGTTCTTATCTTGCAAACA ATAAACACCCATACATATACAGACAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC025140
Insert Size	540 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025140, AAH25140
RefSeq Size	1929 bp
RefSeq ORF	540 bp
Locus ID	26434
Gene Summary	Required for normal acrosome reaction and for normal male fertility (PubMed:12110578, PubMed:15161660, PubMed:15007175). Can bind Cu(2+) (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.