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Nfkb2 (BC027423) Mouse Untagged Clone
CAT＃: MC206386
Nfkb2 (untagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100 (cDNA clone MGC:35934, (10ug)
CNY 2,000.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | lyt, p49, p52, p50B, p49/p100, NF-kappaB2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC027423
GGCGGGCGTCCGAAACTCTGGAAAGCTGAACCGGGGCCCGAAGCCGCAAGACACAGCAGGGCCTAGCCCA GAGATATGGACAATTGCTACGATCCAGGCCTGGATGGCATCCCCGAATATGATGATTTTGAATTCAGCCC CTCCATCGTGGAGCCTAAGGATCCAGCCCCTGAGACAGCTGATGGCCCCTATCTGGTGATTGTGGAACAG CCCAAACAGCGAGGCTTCAGATTTCGATATGGCTGTGAAGGCCCCTCCCATGGAGGTTTGCCAGGTGCCT CCAGTGAGAAGGGCCGGAAGACCTATCCTACTGTCAAGATCTGTAACTATGAGGGACCGGCCAAGATTGA GGTGGACCTGGTGACACACAGTGACCCACCTCGTGCGCATGCCCACAGTCTGGTGGGCAAGCAGTGTTCA GAGTTGGGAGTGTGCGCTGTGTCTGTAGGACCCAAGGACATGACTGCTCAATTTAATAATCTGGGTGTCC TGCATGTAACCAAGAAGAACATGATGGAGATTATGATCCAGAAACTTCAGAGGCAGCGTCTCCGCTCCAA GCCTCAGGGCCTTACAGAGGCTGAGCGGCGGGAGCTAGAGCAGGAGGCCAAGGAGCTGAAGAAAGTCATG GATCTGAGCATTGTACGGCTGCGCTTCTCAGCTTTCCTTCGAGCTAGCGATGGCTCCTTCTCCTTGCCCC TGAAGCCTGTGATCTCCCAGCCCATCCATGACAGCAAGTCTCCAGGGGCCTCGAACCTGAAGATCTCCCG AATGGACAAGACAGCGGGTTCCGTGCGCGGTGGAGACGAAGTTTATTTGCTCTGTGATAAGGTGCAAAAA GACGACATTGAGGTTCGGTTCTATGAGGATGATGAGAATGGATGGCAAGCCTTTGGGGACTTCTCTCCCA CAGACGTTCATAAACAGTATGCCATTGTGTTCCGGACACCGCCCTATCACAAGATGAAGATCGAGAGGCC TGTAACAGTGTTCCTGCAGCTGAAACGCAAGCGTGGGGGCGATGTCTCGGACTCCAAACAGTTCACATAT TACCCTCTGGTGGAAGACAAGGAGGAAGTGCAGAGGAAGCGGAGAAAGGCCTTGCCCACCTTCTCCCAGC CCTTCGGGGGCGGATCCCACATGGGTGGAGGTTCTGGGGGCTCCGCTGGGGGTTATGGAGGCGCTGGAGG AGGTGGCAGCCTCGGCTTTTTCTCCTCCTCCTTGGCCTACAACCCCTACCAATCCGGTGCAGCCCCAATG GGCTGTTATCCGGGTGGGGGAGGTGGAGCGCAGATGGCCGGTTCTAGACGGGACACCGATGCTGGCGAGG GGGCAGAGGAGCCCAGGACGCCCCCGGAGGCTCCCCAGGGCGAACCACAGGCCCTTGACACACTGCAGCG AGCTCGCGAGTACAACGCGCGCCTGTTCGGTCTGGCGCAGCGCAGCGCCCGAGCGTTGCTGGACTACGGC GTCACCGCAGACGCGCGTGCTCTGCTAGCGGGACAGCGCCACCTGCTGATGGCACAGGACGAGAACGGAG ACACGCCACTGCACCTGGCCATCATCCATGGGCAGACTGGTGTCATTGAGCAGATAGCCCACGTCATTTA TCACGCTCAGTACCTCGGCGTCATCAACCTCACCAACCACCTGCACCAGACGCCTCTGCACCTGGCGGTA ATCACTGGGCAGACAAGGGTGGTGAGCTTCCTGCTGCAGGTGGGTGCAGACCCCACGCTGCTGGATCGGC ACGGAGACTCCGCCCTCCACTTGGCTCTCCGGGCAGGTGCTGCAGCCCCAGAGCTGTTGCAGGCACTGTT GCGCAGCGGAGCCCATGCTGTGCCCCAAATATTGCACATGCCTGATTTTGAGGGACTATACCCTGTACAC CTGGCAGTCCATGCCCGAAGCCCTGAGTGCCTGGATCTGTTAGTTGACTGTGGAGCTGAAGTGGAGGCCC CAGAGAGGCAAGGGGGCCGAACTGCCCTGCATCTAGCCACAGAGATGGAGGAGTTGGGGCTGGTCACCCA TCTAGTCACCAAGCTCCATGCTAATGTGAATGCCCGGACCTTTGCTGGAAACACACCCCTCCACCTGGCA GCTGGACTCGGGTCCCCAACTCTTACTCGCCTCCTTCTAAAGGCTGGTGCTGACATCCATGCAGAGAATG AGGAGCCTCTGTGCCCGCTGCCCTCACCCTCGACCTCTGGGAGCGACTCCGACTCTGAAGGGCCTGAGAG GGATACCCAAAGAAACTTCCGAGGCCATACCCCTCTTGACCTCACTTGCAGTACCAAGGTGAAGACTCTG CTGCTAAATGCTGCTCAGAACACCACGGAGCCACCCCTGGCCCCACCCAGCCCTGCAGGGCCAGGGCTGT CCCTGGGGGATGCAGCCCTGCAGAACCTGGAGCAACTGCTGGATGGTCCCGAAGCCCAGGGCAGCTGGGC AGAGCTGGCAGAGCGACTGGGGTTGAGAAGCCTGGTGGACACATACAGGAAGACCCCGTCTCCCAGCGGC AGTCTCCTTCGTAGTTACAAGCTGGCTGGTGGGGACTTGGTGGGTCTATTGGAGGCCTTGTCTGACATGG GTCTCCATGAGGGAGTCAGGCTGCTGAAAGGTCCTGAGACCCGCGACAAGCTGCCCAGCACAGAGGTGAA AGAAGACAGTGCCTATGGGAGCCAGTCAGTGGAGCAGGAGGCAGAGAAGCTGTGTCCACCCCCTGAGCCT CCAGGAGGGCTCTGCCACGGGCACCCCCAGCCTCAGGTGCACTGAATGGCCCCGGTCAACTTCCACCCAG ATCCCTCTGTACAGCATCCCTGTCTAATCGAAATCTTATTTAAACCTCAAGCCCACATCTCGGTGGGTCA AATAAAGGGGAAGACCCCTCCCCAACTTACGGTAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC027423 |
| Insert Size | 2700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC027423, AAH27423 |
| RefSeq Size | 2918 bp |
| RefSeq ORF | 2700 bp |
| Locus ID | 18034 |
| Gene Summary | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65 (By similarity). In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.[UniProtKB/Swiss-Prot Function] |
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