Bag1 (BC003722) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BAG-1; Rap46
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003722 GGGTTGCAAGGCTGCGCGCCCGGTAGCCACCGCTGCGCTGTGAACAAACGCGGCCTGGCGGGTCGCAGCG CCGCGCGGCGACCGCGAGGCGACCGGGAGCCGCTGGGACCCAGGCTGCGCGCCCCTCGGCCTGCCCGGGA GCCGCGCCAGTCGGAGTCCCGGGCCGAGCGGGGCTTGCCTCCCTCTCAGCGTTCCTCTGTGCGCAGTGCA GCCAGCGGGCATGACCGATCCACCAGGGGCGCCGCCGCCGGCGCCTGCAAGCCGCGGGTGAAGAAGAAAG TCCGGCCCCGCTCTTCTCAGAGCGAGAAGGTAGGGAGCAGCAGCAGGGAATTGACTAGAAGTAAGAAAGT GACCCGTAGCAAGAACGTGACCGGGACCCAGGTAGAGGAGGTGACCAAGATCGAGGAGGCGACCCAAACC GAGGAAGTAACTGTGGCAGAAGAGGTGACCCAGACCGACAACATGGCCAAGACCGAGGAGATGGTCCAGA CGGAGGAAATGGAAACACCCAGACTCAGCGTGATCGTCACCCACAGCAATGAGAGGTATGACCTTCTTGT TACCCCACAGCAAGGTAACAGTGAGCCAGTTGTCCAAGACTTGGCTCAGCTTGTTGAAGAGGCCACAGGA GTTCCACTACCTTTTCAGAAGCTCATATTTAAGGGAAAATCTCTGAAAGAAATGGAAACACCGTTGTCAG CACTTGGAATGCAAAATGGTTGCCGAGTCATGTTAATTGGTGAAAAGAGCAATCCAGAAGAAGAGGTTGA GTTAAAGAAGCTGAAAGATTTGGAGGTATCTGCAGAGAAGATAGCTAACCACCTGCAAGAATTGAATAAA GAGCTTTCTGGCATCCAGCAGGGTTTTCTGGCTAAGGAATTGCAAGCGGAGGCTCTCTGCAAACTTGATA GGAAAGTAAAAGCAACAATTGAGCAATTCATGAAGATCTTGGAGGAGATTGACACAATGGTCCTACCAGA ACAATTTAAAGACAGCAGGCTAAAAAGGAAGAATTTGGTGAAAAAGGTTCAGGTGTTCTTAGCAGAGTGT GACACAGTGGAGCAATACATCTGCCAAGAGACAGAGCGGCTGCAGTCTACAAACTTGGCCCTGGCTGAAT GAAGTGCAGTGGAGAGTGGCTGTACTGGCCTGAAGAGCAGCTTTACAGCCCTGCCCTCTCTGGAACAGAA GTCGCCTGTTTCTCCATGGCTGCCAGGGGCAACTAGCCAAATGTCAATTTCCCTGCTCCTCCGTCGGTTC TCAATGAAAAAGTCCTGTCTTTGCAACCTGAATTAGACTTGTGTTTTCTCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC003722
Insert Size	1068 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003722, AAH03722
RefSeq Size	1382 bp
RefSeq ORF	1068 bp
Locus ID	12017
Gene Summary	The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to Bcl2 protein and is referred to as Bcl2-associated athanogene. It enhances the anti-apoptotic effects of Bcl2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least two protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site and an alternative, downstream, AUG translation initiation site. [provided by RefSeq, Jul 2008]

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