Ddx23 (BC049928) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110082M05Rik; 4921506D17Rik
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC049928, the custom clone sequence may differ by one or more nucleotides CGCGAAAGATGGCGACTGTTCGGCGACGTTGAGGCCGCGTTAGGCGGTTCAGGCGCGGGGTGATGGCAGG AGAACTGGCTGACAAAAAGGACCGAGACGCATCACCCAAGGAGGAAAGGAAGCGATCACGGACTCCTGAG AGAGATCGCGATAGAGACCGGGACCGGAAGTCCTCCCCATCTAAAGATAGGAAGCGGCATCGCTCACGGG ATAGGCGTAGAGGAGGGAGCCGTTCTCGCTCTCGGTCCCGTTCCAAGTCTGTAGAGAGGGAACGGCGGCA CAAAGAGCGGGAGCGAGATAAGGAACGGGACCGGAGTAAGAAGGAGCGGGAGCGGGACAAGGATGGCCAC CGACGGGACAAGGACCGAAAAAGATCCAGCTTGTCTCCTGGCCGAGGAAAGGATTTCAAGTCTCGGAAGG ACAGAGATTCTAAGAAGGGTGACGAGGATGAACATGGAGACAAGCGACCTAAGATCCAGCCACTGTCCCT GGAGGAACTTCTGGCCAAGAAAAAGGCTGAGGAGGAAGCTGAAGCTAAGGTAAGGCTGTGGCATCTGGAT TCATGTGCTCTGGCTGTTCTAACAAATGCCACAACTGGGCAGCTTCAGCGTTGGAGCTCTAGGACTGAGA AGTCTCGGCTGCAGTACCAGTGGTTTCCACTGAGGCCCGTTCACAGATGCCGATGCCGATGCCTTTTCTG TCCTTACAGTCACCTCTGTGGGCTCGTCTCCTCGGTATTGATACGTTATTTTCTCATGGAACTACCATGC CAGGAGGCTGCTGTGTTCCCACCATGCCACCTGACTGGCTGTACCTGCAGTCCATGCCAGAGTCCTAGGC CTCTATGTAGTTTTATCAGAATATTTACTATGTTAAGTGAATATTTAGGGCCTTCCTGGGTCTCTGTGGC AGTGATGTGGCTAGATGCTGTTAACCATTCCCTTCTGTCTTGAAGCCCAAATTCCTCTCTAAAGCAGAGC GGGAGGCTGAAGCTCTGAAGCGGCGGCAGCAGGAGGTGGAGGAGCGCCAGAAGATGCTCGAAGAGGAGCG GAAGAAAAGGAAACAGTTCCAGGACCTGGGCAGGAAGATGCTGGGTTTGTTTTCTGTCCTGAGTAGAAAG GGTGGGGTGAAGTTATGGAGAGGGATAGAAATTAGGCTATAGCTCCTGGTAGAACTTGCCTGCCATTCAA AAGGCCCTGGGTCTGATCGCCAACCATTGCAAAAAGAATGGGAGGGCGGAAGATGGAGACTTTTTGTTCC AGACTTTGGAATGGTGAAAGGTCATGGAAATGTGTTAGGTCCCCTTCCCACCCCACGTCCATTCGCTTTC CTCCCATGTCCTGATTGCCTGCATGCTTTCCGAGCCTCTCCCTCCCACGGAGCTCTTCCACAGCTGTTCT TCTTTGTCTGCAGAAGATCCTCAGGAAAGGGAACGGAGAGAACGGAGGGAAAGGATGGAGCGGGAGACCA ACGGAAATGAGGATGAGGAGGGGCGGCAGAAGATCCGGGAAGAAAAGGACAAGAGCAAGGAGCTGCACGC CATTAAGGAGCGTTACCTGGGTGGCATCAAAAAACGGCGCCGAACAAGGCATCTCAATGACCGAAAGTTT GTGTTTGAGTGGGATGCGTCTGAAGACACCTCCATTGATTACAACCCCCTGTATAAAGAACGACACCAGG TGCAGCTGTTGGGGCGAGGCTTCATTGCAGGCATTGACCTAAAGCAGCAGAAGCGAGAGCAGTCACGTTT CTATGGAGACCTGATGGAGAAGAGGCGAACCCTGGAGGAAAAGGAGCAGGAGGAAGCAAGACTCCGCAAA CTTCGCAAAAAGGAGGCCAAGCAACGCTGGGATGACCGCCATTGGTCCCAGAAGAAGTTGGATGAGATGA CGGACAGGGACTGGCGGATCTTCCGTGAAGATTACAGCATCACCACCAAAGGTGGCAAGATCCCCAATCC CATCCGGTCCTGGAAAGATTCGTCTCTGCCCCCTCATATCCTGGAAGTCATTGATAAGTGTGGCTACAAG GAGCCAACACCTATCCAGCGTCAGGCCATTCCCATTGGGCTGCAGAATCGTGACATCATTGGTGTAGCTG AGACTGGCAGTGGCAAGACAGCAGCCTTCCTCATCCCATTGCTAGTCGGGATCACCACACTTCCCAAGAT TGACAGGATCGAAGAGTCAGACCAGGGCCCTTATGCCATCATCCTGGCTCCTACCCGTGAGTTGGCTCAG CAGATTGAGGAAGAAACCATCAAGTTCGGAAAGCCACTCGGTATCCGCACTGTGGCTGTCATCGGTGGCA TCTCCAGAGAAGACCAAGGCTTCCGGCTACGCATGGGCTGTGAGATTGTGATCGCCACCCCGGGGCGCCT GATCGATGTGCTGGAGAACCGGTACCTGGTGCTGAGCCGCTGCACCTACGTGGTTCTGGATGAGGCGGAT AGGATGATTGACATGGGCTTTGAGCCAGATGTGCAGAAGATCCTGGAACACATGCCTGTCAGCAACCAGA AGCCGGACACAGATGAGGCTGAGGACCCGGAGAAGATGTTGGCCAACTTTGAATCAGGAAAACATAAATA CCGTCAAGTAAGGCGGCTTTCTTGGGAGAAGCTGAACCAGTTCCCAAGTCTTGTTTGCAGACCTTCCTTG CCCAGGGAGGGCCCAGTGCACATGCCTCCAGCATGCTAGCCGTGCCTGTGTACAGTCCTCTGACTGCCGA GGGGTAGAGCCATGTAGCTTAGCCTAAAAAGCTGATTCTAGAGAATCATGCTACTGCCAAGGAAGGAAAA AGTGCCAGGCATAGTGGTTCCTGCCAAAGCCCCAGCATTCAGGGGGAGGTGGAATATGGGGACTGAGCTA GCTTGTCTGATACGTGAGCTCCAGGCCAGTCAGATCTATGTAGCAAGATCCTGTCTCAAAAAAGTAAACA ACAGTAAGGACTGAGGGGCTGGTGAGCCAGCTCAGGAGTGAGGAGCACTGGTTGCCCTTCCAGAGTTCAG TTCCCAGCACCCACTTGGTGGCATCTAGAAAGGGCTCTGATGTGTAGGTGTACAGAGAGCGCTCATGTAT GAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC049928
Insert Size	1221 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC049928
RefSeq Size	3096 bp
RefSeq ORF	1221 bp
Locus ID	74351

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