Rad9 (BC082556) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Rad9
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC082556 AAGCTCGATTTTTGTGCTTATGTACTATGAAGTGCCTGATCACCGGGGGCAACGTGAAGGTGCTGGGCAA GGCTGTCCATTCGCTATCCCGAATCGGGGACGAGCTCTATCTGGAACCCTTGAAGGACGGGCTCTCCCTA CGGACTGTGAACTCGTCCCGTTCTGCCTATGCCTGCTTCCTTTTTGCCCCACTCTTCTTCCAGCAGTACC AGGCGGCTTCCCCTGGTCAGGACCTGCTGCGCTGTAAGATCCTGATGAAGGCCTTCCTGTCCGTCTTCCG CTCTCTGGCAATTGTGGAGAAGTCTGTGGAGAAGTGCTGTATCTCCCTCAGTGGCAGCCACAGCCACCTG GTGGTCCAGCTCCACTGCAAGTATGGGGTCAAGAAGACACACAACCTCTCCTTCCAGGACTGTGAGTCCC TGCAGGCTGTCTTCGACCCAGCCTCATGCCCTCACTTATTGCGCACCCCAGCACGGGTTCTGGCAGAGGC TGTTCTGTCCTTTCCCCTTGCATTGACTGAGGTGACACTGGGCATTGGCCGTGGCCGGCGGGTCATCCTG CGCAGCTACCAGGAAGAGGAGGCAGACAGCACCAGCAAAGCCATGGTGACTGAGACCAGCATTGGGGATG AGGACTTCCAGCAGCTGCATGCCCCAGAAGGGATAGCTGTCACCTTCTGCCTCAAGGAATTTCGGGGGCT CCTGAGCTTTGCAGAGTCAGCGAATTTGCCTCTTACTATCCACTTCGATGTTCCAGGCAGGCCAGTCATC TTTACTATTGAGGATTCCTTGCTGGATGCCCACTTTGTCTTGGCCACACTCTTAGAGCAAGACTCATGTT CCCAGGGCCCGTGTTCCCCAAAACCCCACCAGCCAGTGCCTCAGAAGCAGGCACACAGCACACCCCACTT AGATGACTTTACCAGTGATGACATTGACTGTTACATGATTGCCATGGAAACCACTGGAGGCAATGAGGGC TCCGGGGCACAGCCTTCCACATCCCTCCCACCTGTCTCCCTGGCCTCCCATGACCTTGCCCCCACCTCAG AGGAGGAAGCTGAGCCCAGTACAGTGCCTGGGACTCCCCCACCCAAGAAGTTTCGTTCACTGTTCTTTGG CTCCATCCTGGCCCCTGTACACTCCCCGCAGGGTCCCAACCCTGTGCTGGCTGAAGACAGTGATGGTGAA GGGTGAATTTGAGAAATTAAAGTCTGTGTTCAAAGGCTCACGGACCAGAAGCAGGCCCGCCCACTGAGTT GGGCGTCAGTCCTTAGACATGGACATGGAAATGGAAAAAGGCATACCCCACTTGGCTGTCTGCTGCAGAG CTGCCTAGGAACCATGTTTGGTCTGTGTTCTGTATTTATTCAGCCTATACCAATCATGTCTCTGGTGCCC ACCTGTCACCTGACAGATCCCTCATCCTTTACTTTGAGGCTCTAGAATACAAATTCTGGCCAAAGCCAGA AACTTAGCTTCCTCAGTCTCCTGCCTCTAAAACAGGAAGATCAGTATTGAACACCCTCTGCCAATCATGA ACTCCCCGCCAGACCCTCCTGTGGGCCAAAGGAACTGTTTCTCTTTCCTAACACTTGGGCCTTGGCCTAG AATCCTAATAGCCTCTGGACCTGATTGTGTGTCACTGGTGTTGCCCTGGCTGGGCCCATGTTCAGCCACT CAGTCTGCCCTCCCAGGTGGCTTTGTACCAAAGAACTGCTGGCCCACTGTCTGGGACGGGACAAGAGAGA CAGAGGCTATGGTGAGAATCCAGCTTTGACCTTTATTCAAGAGACCAGATGGGTTGCCCCAGGATCTGGC TGCCAGCCATGCAAGGCTGGAGACCCACAATCTGGTCTGCCATTGCCCTGAGCTGCAGCCTTGGCCCCAG GATTCCACTTGCAGCCACCACAGGTGCTGGTGGGAGGGAGCCCTGGGAGATTAGATGCTGCTATTGATTC TTTAAAAAATAAAGGAAAGACACTTAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC082556
Insert Size	1170 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC082556, AAH82556
RefSeq Size	2006 bp
RefSeq ORF	1170 bp
Locus ID	19367
Gene Summary	Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity).[UniProtKB/Swiss-Prot Function]

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