Rraga (NM_178376) Mouse Untagged Clone
CAT＃: MC207666
Rraga (untagged) - Mouse Ras-related GTP binding A (Rraga), (10ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1300010C19Rik; AI255374; FIP-1; RAGA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207666 representing NM_178376
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAATACAGCCATGAAGAAAAAGGTGCTGTTGATGGGAAAGAGCGGGTCGGGGAAGACCAGCATGA GGTCGATCATCTTTGCAAATTATATTGCGCGCGACACTAGGCGCCTGGGTGCCACCATCGACGTGGAGCA CTCCCACGTCCGATTCTTGGGGAACCTGGTGCTGAACCTGTGGGACTGTGGCGGCCAGGACACCTTTATG GAAAATTACTTCACCAGCCAGCGGGACAACATCTTCCGTAATGTGGAGGTTCTGATATACGTGTTTGACG TAGAGAGCCGCGAACTGGAAAAAGATATGCATTATTACCAATCGTGTCTGGAAGCCATCCTCCAGAATTC ACCTGACGCCAAAATCTTCTGCCTGGTGCACAAAATGGATCTGGTTCAGGAAGATCAACGTGACCTGATT TTTAAAGAGCGAGAGGAAGACCTGAGGCGTTTGTCTCGCCCGCTGGAGTGCGCTTGTTTTCGAACGTCCA TCTGGGATGAGACGCTCTACAAAGCCTGGTCCAGCATCGTCTATCAGCTGATCCCCAACGTTCAGCAGCT GGAGATGAACCTAAGGAATTTTGCCCAGATTATTGAGGCTGATGAAGTTCTGCTGTTCGAGAGAGCTACG TTCTTGGTGATTTCCCACTACCAGTGCAAAGAGCAGCGAGATGTGCACCGCTTTGAGAAGATCAGCAACA TCATCAAGCAGTTTAAGCTGAGCTGCAGTAAATTGGCCGCTTCTTTCCAGAGCATGGAAGTGAGGAATTC TAACTTCGCTGCCTTCATCGACATCTTTACATCAAACACGTACGTGATGGTGGTCATGTCAGATCCATCC ATCCCTTCTGCAGCTACTCTGATCAACATTCGTAATGCCAGGAAACACTTTGAGAAGCTAGAAAGAGTAG ATGGGCCCAAGCACAGCCTCCTCATGCGTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_178376 |
| Insert Size | 942 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178376.3, NP_848463.1 |
| RefSeq Size | 1653 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 68441 |
| UniProt ID | Q80X95 |
| Gene Summary | Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204448 | Rraga (tGFP-tagged) - Mouse Ras-related GTP binding A (Rraga) |
CNY 2850.00 |
|
| MR204448 | Rraga (Myc-DDK-tagged) - Mouse Ras-related GTP binding A (Rraga) |
CNY 2400.00 |
|
| MR204448L3 | Lenti ORF clone of Rraga (Myc-DDK-tagged) - Mouse Ras-related GTP binding A (Rraga) |
CNY 4750.00 |
|
| MR204448L4 | Lenti ORF clone of Rraga (mGFP-tagged) - Mouse Ras-related GTP binding A (Rraga) |
CNY 4750.00 |
