Sftpb (NM_147779) Mouse Untagged Clone

CAT#: MC209384

Sftpb (untagged) - Mouse surfactant associated protein B (Sftpb), (10ug)



  "NM_147779" in other vectors (4)

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI562151; SF-B; Sftp-3; Sftp3; SP-B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209384 representing NM_147779
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAGTCGCACCTACTGCAGTGGCTACTGCTGCTTCCTACCCTCTGCTGCCCAGGTGCAGCTATCA
CGTCGGCCTCATCCCTGGAGTGTGCACAAGGCCCTCAATTCTGGTGCCAAAGCCTGGAGCATGCAGTGCA
GTGCAGAGCCCTGGGGCACTGCCTGCAGGAAGTCTGGGGGCATGCAGGAGCTAATGACCTGTGCCAAGAG
TGTGAGGATATTGTCCACCTCCTCACAAAGATGACCAAGGAAGATGCTTTCCAGGAAGCAATCCGGAAGT
TCCTGGAACAAGAATGTGATATCCTTCCCTTGAAGCTGCTTGTGCCCCGGTGTCGCCAAGTGCTTGATGT
CTACCTGCCCCTGGTTATTGACTACTTCCAGAGCCAGATTAACCCCAAAGCCATCTGCAATCATGTAGGC
CTGTGCCCACGTGGGCAGGCTAAGCCAGAACAGAATCCAGGGATGCCGGATGCCGTTCCAAACCCTCTGC
TGGACAAGCTGGTCCTCCCTGTGCTGCCAGGAGCCCTCTTGGCAAGGCCTGGGCCTCACACTCAGGACTT
CTCTGAGCAACAGCTCCCCATTCCCCTGCCCTTCTGCTGGCTTTGCAGAACTCTGATCAAGCGGGTTCAA
GCCGTGATCCCCAAGGGTGTGCTGGCTGTGGCTGTGTCCCAGGTGTGCCACGTGGTACCCCTGGTGGTGG
GTGGCATCTGCCAGTGCCTGGCTGAGCGCTACACAGTTCTCCTGCTAGACGCACTGCTGGGCCGTGTGGT
GCCCCAGCTAGTCTGTGGCCTTGTCCTCCGATGTTCCACTGAGGATGCCATGGGCCCTGCCCTCCCTGCT
GTGGAGCCTCTGATAGAAGAATGGCCACTACAGGACACTGAGTGCCATTTCTGCAAGTCTGTGATCAACC
AGGCCTGGAACACCAGTGAACAGGCTATGCCACAGGCAATGCACCAGGCCTGCCTTCGCTTCTGGCTAGA
CAGGCAAAAGTGTGAACAGTTTGTGGAACAGCACATGCCCCAGCTGCTGGCCCTGGTGCCTAGGAGCCAG
GATGCCCACATCACCTGCCAGGCCCTTGGCGTATGTGAGGCCCCGGCTAGCCCTCTGCAGTGCTTCCAAA
CCCCACACCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_147779
Insert Size 1134 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_147779.2, NP_680088.1
RefSeq Size 1564 bp
RefSeq ORF 1134 bp
Locus ID 20388
UniProt ID P50405
Gene Summary Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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