Nfia (NM_001122953) Mouse Untagged Clone
CAT＃: MC216272
Nfia (untagged) - Mouse nuclear factor I/A (Nfia), transcript variant 3, (10ug)
CNY 6080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110047K16Rik; 9430022M17Rik; CTF; NF1-A; NF1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216272 representing NM_001122953
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTCTCCGCTCTGTCTCACCCAGGATGAGTTTCATCCTTTCATTGAAGCACTTCTGCCCCATGTCC GCGCCTTCGCCTACACATGGTTCAACCTGCAGGCCCGAAAGCGGAAATACTTCAAAAAACATGAGAAGCG CATGTCGAAAGAAGAGGAGAGGGCCGTGAAGGATGAACTGCTAAGCGAGAAGCCCGAGGTCAAGCAGAAG TGGGCTTCCCGACTTCTGGCCAAGTTACGGAAAGATATCCGACCCGAGTACCGAGAGGATTTTGTTCTTA CAGTTACAGGGAAAAAACCTCCATGCTGTGTTCTTTCCAACCCTGATCAGAAAGGCAAGATGCGGAGAAT TGACTGCCTCCGCCAGGCAGATAAAGTATGGAGGTTGGACCTCGTCATGGTGATCTTGTTCAAAGGTATT CCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGAGTCCACAGTGCTCTAATCCAGGGCTCTGTGTCC AGCCCCATCACATAGGGGTTTCTGTAAAGGAACTCGATTTATATTTGGCATACTTTGTACATGCAGCAGA TTCAAGTCAATCTGAAAGTCCCAGCCAGCCAAGTGAAGCTGACATTAAGGACCAGCCAGAAAATGGACAT TTGGGCTTCCAGGACAGCTTTGTCACATCAGGTGTTTTCAGTGTGACTGAGCTAGTAAGAGTGTCACAAA CACCAATAGCTGCAGGAACCGGCCCCAATTTTTCTCTCTCTGATTTGGAAAGTTCTTCATACTACAGCAT GAGTCCAGGAGCAATGAGGAGGTCTCTGCCCAGCACATCCTCTACCAGCTCTACAAAGCGCCTCAAGTCT GTGGAGGATGAAATGGACAGTCCTGGTGAAGAACCATTTTACACAGGCCAAGGGCGCTCCCCAGGGAGTG GCAGCCAGTCCAGTGGATGGCATGAAGTAGAGCCAGCAAGTCCACATGCGACGCCATCGACTCTCCACTT TCCAACGTCACCCATCATCCAGCAGCCTGGGCCTTACTTCTCACACCCAGCCATCCGTTACCACCCTCAG GAGACGCTGAAAGAGTTTGTCCAACTTGTCTGTCCTGATGCTGGTCAGCAAGCTGGACAGGTGGGGTTCC TCAATCCCAATGGAAGCAGTCAAGGCAAGGTGCACAACCCATTCCTCCCCACCCCAATGTTGCCGCCGCC GCCACCACCACCGATGGCCAGGCCTGTGCCTCTGCCCATGCCAGACACCAAGCCTCCAACCACATCAACA GAAGGAGGTGCAGCCTCCCCCACCTCACCGACCTACTCGACACCCAGCACCTCCCCCGCAAACCGATTCG TCAGTGTTGGACCACGGGATCCAAGCTTTGTAAATATCCCTCAACAGACACAGTCCTGGTACCTGGGATA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001122953 |
| Insert Size | 1401 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001122953.1, NP_001116425.1 |
| RefSeq Size | 9399 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 18027 |
| UniProt ID | Q02780 |
| Gene Summary | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has an alternate 5' exon and lacks an internal coding exon, which does not affect the reading frame, as compared to variant 1. The resulting isoform (2) has a different and shorter N-terminus and lacks an internal segment, as compared to isoform 1. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225705 | Nfia (tGFP-tagged) - Mouse nuclear factor I/A (Nfia) transcript variant 3, (10ug) |
CNY 5130.00 |
|
| MR225705 | Nfia (Myc-DDK-tagged) - Mouse nuclear factor I/A (Nfia), transcript variant 3 |
CNY 5488.00 |
|
| MR225705L3 | Lenti ORF clone of Nfia (Myc-DDK-tagged) - Mouse nuclear factor I/A (Nfia), transcript variant 3 |
CNY 6560.00 |
|
| MR225705L4 | Lenti ORF clone of Nfia (mGFP-tagged) - Mouse nuclear factor I/A (Nfia), transcript variant 3 |
CNY 6560.00 |
