Blk (NM_007549) Mouse Untagged Clone
CAT＃: MC216930
Blk (untagged) - Mouse B lymphoid kinase (Blk), (10ug)
CNY 6460.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216930 representing NM_007549
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGCTGAGCAGCAAGAGGCAGGTCAGTGAGAAGGGCAAGGGCTGGAGCCCCGTGAAGATCCGCA CCCAGGACAAGGCTCCCCCACCCCTGCCACCCCTGGTTGTCTTCAACCACCTTGCCCCACCATCTCCTAA CCAGGACCCAGATGAAGAGGAGCGTTTTGTGGTGGCTCTGTTTGACTATGCCGCTGTGAATGACAGGGAC CTTCAGGTGCTGAAGGGTGAGAAGCTCCAGGTCTTGAGGAGCACTGGAGACTGGTGGTTGGCCAGGTCAC TCGTCACAGGAAGAGAAGGTTATGTGCCCAGCAACTTTGTGGCCCCAGTAGAGACTCTGGAAGTAGAAAA ATGGTTCTTCAGGACCATCAGCCGGAAGGATGCTGAGAGGCAGTTGCTGGCTCCGATGAACAAGGCCGGC TCCTTTCTCATCAGAGAGAGTGAGAGCAATAAAGGTGCCTTTTCCCTGTCCGTGAAAGATATCACCACCC AGGGGGAGGTGGTCAAGCACTATAAGATCCGATCACTGGACAATGGAGGCTATTACATCTCCCCCCGGAT CACCTTTCCCACCCTCCAGGCCCTGGTGCAGCACTATTCAAAGAAAGGGGATGGTTTGTGTCAGAAGTTG ACTCTGCCCTGTGTGAACCTGGCCCCGAAGAACCTTTGGGCCCAAGATGAATGGGAAATCCCCAGGCAGT CTCTCAAGTTGGTCCGGAAACTTGGGTCTGGGCAGTTTGGCGAAGTCTGGATGGGTTATTACAAAAATAA CATGAAGGTGGCCATCAAGACCCTGAAGGAGGGAACCATGTCACCGGAAGCTTTCCTGGGCGAGGCCAAC GTGATGAAAACCCTGCAGCATGAGAGGCTGGTTCGTCTCTACGCTGTGGTCACCAGAGAGCCCATTTACA TCGTCACTGAATACATGGCCAGAGGATGCTTGCTGGATTTTCTGAAGACCGATGAAGGTAGCAGGTTGTC CCTTCCAAGGCTGATTGACATGTCAGCCCAGGTTGCAGAGGGGATGGCTTACATAGAGCGCATGAATTCC ATCCACCGTGACCTGCGGGCAGCCAACATCCTGGTGTCTGAGACGTTGTGCTGCAAAATCGCTGACTTCG GCTTGGCCAGGATCATTGACAGTGAATACACTGCCCAAGAGGGGGCCAAGTTCCCCATCAAGTGGACCGC CCCGGAGGCCATCCACTTCGGGGTGTTTACCATCAAGGCTGATGTGTGGTCCTTCGGAGTCTTGCTGATG GAGATTGTCACCTATGGGCGCGTTCCCTACCCAGGAATGAGCAACCCTGAGGTCATCCGTAGCCTGGAGC ACGGCTACCGAATGCCATGCCCGGAGACATGTCCACCGGAGTTGTACAATGATATCATCACTGAGTGCTG GCGGGGCCGGCCAGAGGAGCGGCCTACCTTTGAGTTCCTGCAGTCGGTGTTGGAGGACTTCTACACAGCC ACGGAGGGCCAATATGAGCTGCAGCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007549 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007549.2, NP_031575.2 |
| RefSeq Size | 2475 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 12143 |
| UniProt ID | P16277 |
| Gene Summary | Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors (PubMed:2404338, PubMed:7690139, PubMed:7608542, PubMed:9636152, PubMed:14662906, PubMed:12563261). Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation (PubMed:2404338, PubMed:7690139, PubMed:7608542, PubMed:14662906, PubMed:12563261). Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor (PubMed:2404338, PubMed:7690139, PubMed:7608542, PubMed:14662906, PubMed:12563261). Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207' (PubMed:7592958, PubMed:9177269). Phosphorylates also the immunoglobulin G receptor FCGR2 (By similarity). With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation (PubMed:14662906, PubMed:12563261). Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation (PubMed:7565679). In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose (By similarity). Phosphorylates CGAS, promoting retention of CGAS in the cytosol (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208004 | Blk (tGFP-tagged) - Mouse B lymphoid kinase (Blk) |
CNY 3710.00 |
|
| MR208004 | Blk (Myc-DDK-tagged) - Mouse B lymphoid kinase (Blk) |
CNY 3656.00 |
|
| MR208004L3 | Lenti ORF clone of Blk (Myc-DDK-tagged) - Mouse B lymphoid kinase (Blk) |
CNY 5230.00 |
|
| MR208004L4 | Lenti ORF clone of Blk (mGFP-tagged) - Mouse B lymphoid kinase (Blk) |
CNY 5230.00 |
