Dnajc3 (NM_008929) Mouse Untagged Clone
CAT#: MC217248
Dnajc3 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 3 (Dnajc3), (10ug)
CNY 6560.00
Product images

Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AA408985; AU067833; Dnajc3a; Dnajc3b; mp58; p58; p58IPK; Prkri |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC217248 representing NM_008929
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCCCCGGCTCGGTGGGCAGCCGGCTGGGCGCGGTGTTCCCGTTCCTGCTGGTCCTGGTGGACC TGCAGTACGAAGGCGCTGAGTGTGGAGTAAATGCGGATGTGGAGAAGCATCTTGAATTGGGGAAGAAATT ACTCGCAGCCGGACAGCTAGCCGACGCCTTATCACAGTTTCACGCTGCAGTTGATGGTGACCCCGATAAC TATATCGCATACTATAGGAGAGCTACCGTCTTCTTAGCCATGGGAAAATCCAAAGCAGCGCTGCCCGACC TCACCAAAGTGATTGCGCTGAAGATGGACTTTACTGCCGCAAGACTACAGAGAGGTCACCTACTGCTCAA ACAAGGGAAGCTTGACGAAGCAGAAGACGATTTCAAGAAAGTGCTCAAATCTAACCCCAGTGAGCAGGAG GAGAAGGAAGCCGAGTCTCAGCTTGTAAAAGCTGACGAGATGCAGCGGCTGCGCTCCCAAGCGCTCGACG CCTTTGACGGTGCCGATTACACTGCTGCTATAACCTTCCTTGATAAGATTTTAGAGGTTTGTGTTTGGGA TGCAGAACTGCGTGAACTGCGAGCAGAATGCTTCATAAAGGAAGGAGAGCCCAGGAAAGCCATCAGCGAC TTAAAGGCTGCTTCAAAGCTGAAGAGTGACAACACAGAGGCCTTTTACAAAATCAGCACACTCTACTACC AGCTAGGAGACCATGAGCTGTCTCTCAGTGAAGTTCGTGAATGTCTGAAACTTGACCAGGATCACAAGCG GTGTTTTGCACACTATAAGCAAGTGAAGAAACTTAACAAGCTGATTGAGTCAGCTGAAGAGCTGATCCGA GATGGCAGATACACAGATGCAACCAGCAAATATGAATCAGTCATGAAAACAGAGCCCAGTGTTGCTGAGT ACACAGTGCGCTCGAAGGAGAGGATCTGCCACTGCTTCTCTAAGGATGAGAAGCCCGTGGAAGCCATTAG GATATGTTCTGAAGTTTTACAGATGGAGCCCGACAATGTGAATGCTCTGAAAGACCGAGCCGAGGCCTAC TTAATAGAGGAAATGTACGATGAAGCCATTCAGGATTATGAAGCTGCTCAGGAGCACAATGAAAATGACC AGCAGATTCGGGAAGGCTTAGAGAAAGCCCAGCGGTTACTGAAGCAGTCACAGAAACGAGATTATTATAA AATCTTGGGAGTAAAAAGAAATGCCAAAAAACAAGAAATCATTAAAGCATACCGAAAGTTAGCACTGCAG TGGCATCCAGATAATTTCCAGAATGAAGAAGAAAAGAAAAAAGCAGAGAAAAAGTTCATTGACATAGCAG CTGCTAAAGAAGTCCTCTCAGACCCAGAAATGAGAAAGAAGTTTGATGATGGAGAAGATCCTCTTGATGC AGAGAGTCAACAAGGAGGTGGTGGCAACCCCTTCCACAGAAGTTGGAACTCATGGCAAGGGTTTAACCCC TTCAGCTCAGGCGGACCGTTCAGATTTAAGTTCCACTTCAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_008929 |
Insert Size | 1515 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_008929.3, NP_032955.2 |
RefSeq Size | 5190 bp |
RefSeq ORF | 1515 bp |
Locus ID | 100037258 |
UniProt ID | Q91YW3 |
Gene Summary | Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (PubMed:25329545). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG227024 | Dnajc3 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily C member 3 (Dnajc3), (10ug) |
CNY 5510.00 |
|
MR227024 | Dnajc3 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 3 (Dnajc3) |
CNY 6000.00 |
|
MR227024L3 | Lenti ORF clone of Dnajc3 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 3 (Dnajc3) |
CNY 6940.00 |
|
MR227024L4 | Lenti ORF clone of Dnajc3 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 3 (Dnajc3) |
CNY 6940.00 |