Ythdf3 (BC057158) Mouse Untagged Clone
CAT＃: MC218605
Ythdf3 (untagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717), (10ug)
CNY 6940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9130022A11Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC057158
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTATCTTGATTTGACTTTGCTTCATAGGGCAACAGAGGAAACAGGCGAAGAATCATTTTCAGTAC AAAATGGTTCGATTCATCAAAAAGATGCTGTAAATGATGATGATTTTGAGCCATACTTAAGTAGCCAGAC AAATCAGAATAACAGCTATCCACCAATGTCAGATCCATATATGCCTAGTTACTATGCTCCGTCCATTGGA TTTCCATATTCTCTTGGGGAAGCAGCATGGTCCACGGCTGGAGATCAGCCTATGCCATATCTGACAACCT ATGGACAAATGAGTAATGGAGAACATCATTATATACCAGATGGTGTTTTTAGTCAACCAGGGGCATTAGG AAATACCCCTCCATTTCTTGGTCAACATGGATTTAACTTTTTTCCTGGTAATGCTGATTTCTCTACATGG GGAACAAGTGGATCTCAGGGACAATCAACACAAAATTCTGCTTATAGTAGCAGTTACGGCTATCCACCTA GTTCTCTTGGGAGAGCTATTACTGATGGACAGGCCGGATTTGGCAATGATACTTTGAGTAAGGTGCCTGG CATTAGTAGTATTGAGCAAGGCATGACTGGACTGAAAATTGGTGGTGACCTGACAGCTGCGGTGACGAAA ACTGTAGGTACAGCCTTGAGCAGCAGTGGTATGACTAGCATTGCAACCAATAATGTGCCCCCTGTTAGCA GTGCAGCACCTAAACCAACTTCTTGGGCTGCTATTGCCAGAAAGCCTGCAAAACCTCAGCCGAAACTTAA ACCCAAGGGCAATGTGGGAATTGGGGGTTCTGCTGTGCCACCACCTCCTATAAAACACAACATGAATATT GGAACTTGGGATGAAAAGGGGTCAGTGGTAAAGGCTCCACCAACCCAACCAGTTCTGCCTCCTCAAACTA TAATCCAGCAGCCTCAGCCATTAATTCAACCACCACCATTGGTGCAAAGCCAACTGCCTCAACAGCAGCC TCAGCCACCACAACCACAGCAGCAACAAGGACCTCAGCCACAGGCCCAGCCTCACCAAGTGCAGTCTCAA CAGCCACAGTTGCAGAATCGTTGGGTAGCTCCTCGGAATAGGGGAACCGGCTTCAACCAGAACAATGGAA CAGGCAGTGAAAACTTTGGTTTAGGTGTTGTGCCTGTTAGTGCTTCACCTTCTAGTGTAGAGGTGCATCC AGTGCTGGAAAAACTAAAGGCCATAAACAATTATAATCCCAAAGACTTTGATTGGAACCTGAAGAATGGA CGTGTGTTTATAATTAAGAGCTATTCTGAGGATGATATACATCGTTCCATCAAGTACTCTATCTGGTGTA GTACTGAGCATGGTAATAAGCGTTTGGATGCAGCTTATCGTTCCCTGAATGGGAAAGGCCCACTCTATTT ACTCTTCAGTGTGAATGGCAGTGGACATTTTTGTGGAGTGGCTGAAATGAAGTCTGTTGTAGACTATAAT GCTTATGCTGGTGTCTGGTCTCAGGATAAGTGGAAGGGCAAATTTGAAGTTAAATGGATCTTTGTCAAAG ATGTTCCCAATAACCAATTACGACATATTCGCTTAGAAAATAATGACAACAAACCAGTAACCAATTCAAG GGACACTCAAGAAGTACCCCTAGAAAAAGCAAAGCAAGTGCTTAAAATAATCGCTACTTTCAAGCATACC ACCTCAATCTTTGATGACTTTGCACATTATGAAAAGCGTCAAGAGGAGGAGGAAGCCATGCGAAGGGAGA GAAATAGAAACAAGCAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC057158 |
| Insert Size | 1770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057158, AAH57158 |
| RefSeq Size | 3173 bp |
| RefSeq ORF | 1769 bp |
| Locus ID | 229096 |
| Gene Summary | Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs and promotes RNA translation efficiency (By similarity). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (By similarity). Shares m6A-containing mRNAs targets with YTHDF1 and YTHDF2, and regulates different processes depending on the context (By similarity). Facilitates the translation of targeted mRNAs in cooperation with YTHDF1 by binding to m6A-containing mRNAs and interacting with 40S and 60S ribosome subunits (By similarity). Acts as a negative regulator of type I interferon response by down-regulating interferon-stimulated genes (ISGs) expression: acts by binding to FOXO3 mRNAs and promoting their translation (PubMed:30591559). Binds to FOXO3 mRNAs independently of METTL3-mediated m6A modification (PubMed:30591559). Can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation (By similarity). Recognizes and binds m6A-containing circular RNAs (circRNAs) and promotes their translation (By similarity). circRNAs are generated through back-splicing of pre-mRNAs, a non-canonical splicing process promoted by dsRNA structures across circularizing exons (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209195 | Ythdf3 (tGFP-tagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717) |
CNY 6460.00 |
|
| MR209195 | Ythdf3 (Myc-DDK-tagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717) |
CNY 5890.00 |
|
| MR209195L3 | Lenti ORF clone of Ythdf3 (Myc-DDK-tagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717) |
CNY 7790.00 |
|
| MR209195L4 | Lenti ORF clone of Ythdf3 (mGFP-tagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717) |
CNY 7790.00 |
