Limd1 (NM_013860) Mouse Untagged Clone
CAT＃: MC220222
Limd1 (untagged) - Mouse LIM domains containing 1 (Limd1), (10ug)
CNY 8550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW822033; D9Ertd192e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220222 representing NM_013860
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAAATACGATGATCTGGGCCTTGAGGCCAGTAAGTTCATCGAGGACCTGAACATGTATGAGGCCT CCAAGGATGGGCTCTTCCGGGTGGACAAGGGTGCTGGCAACAACCCAGAATTTGAGGAAACTCGAAGGGT GTTCGCGACCAAGATGGCCAAAATCCACCTCCAGCAGCAGCAACAGCAGCAGCTCCTACAGGAGGAGGCC CTACCTAGGGCAGGCAGAAGCCCGGTCAACGGTGGGAACCGTCAGGGTGCGAGCGGCAAGCTGGCTGCAG ATGGGGCCGCTAAGCCTCCTCTGGCTGTGCCAACAGTGGCACCTGGATTAGCTACTACCACTGCGGCCGC GCAGCCCTCGTACCCATCTCAGGAGCAGAGGATCAGGCCATCTGCCCATGGTGCAAGGCCTGGCAGTCAG AACTGTGGTTCCAGGGAGGGGCCTGTGAGTTCCCAGAGACCTGCTTTACACGGCCTGAGTCCCTCCTGTG AAGACCCTTCCTGCCTCACTCACGGAGACTATTATGACAATTTCTCTTTGGCAAGCCCACAGTGGGGTGA TAAACCTGAAGGGTGCCCCAGCGTGAGTCTGGGTGTAGGAAGTGGATGGCCTGGTTGCCCAGGGAATGAC TCCACATTGCCCAAATCCTGTGGAGACCATCACCCTTACCAGCCACAGCTCTCCACAGTGTGCTCTGGCA GGTCTTTTGAAAGTGGCATCAGTGGCCAGGACGGTGGCATTGGTGGCCATAGCAGCGAGAAGCCAACAGG CCTTTGGTCCACTGCCTCCTCTCAGCGAGTGAACCTCGGTTTTTCTTCCATGGGCTTGGAGAATGGGACC TCCGCTCAACCCAAGGGCACAACTGTTTCAGCACCGATGGTCCCTAGCAGCGCCAGCCAAGGAGCTTGTC CGAAAAGAGATTCAGGTCTGGGATATGAGGCTTCAGGCAGGGTCTTCAAACCCCTTGTGGACACTCAGCC TTGGCTGCAGGATGGGCCCAAGTCTTACCTCTCAGTTTCTGCTCCATTATCCTCGACAGCTGGCAAGGAC AGTACCCAGCCAGGTATGACCACCGGGCTGGATCCTAAGTTTGGATGCGTGGAGTCTGGCACTAGTCCCA AGCCCAGCCCCACCAGTAACGTCCATCCGGTAATGTCCACTCCATCTGAGTTATCTTGTAAAGAGAGTTC TCCCAGCTGGTCCACTGACAGTAGTCTGGAACCTGTGCTCCCAGGGAGTCCCACCCCCTCCAGGGTGAGA TTGCCCTGCCAGACCCTCGCACCGGGCCCTGAGCTTGGACCCTCCACTGCGGAATTGAAGTTGGAAGCCC TCACCCAGCGTCTGGAGCGAGAGATGGATGCTCACCCCAAAGCGGACTACTTCGGTTCCTGTGTGAAATG CAGCAAAGGGGTGTTTGGAGCTGGCCAGGCCTGTCAGGCCATGGGAGATCTCTACCACGATGCGTGCTTC ACCTGTGCAGCCTGCAGCAGGAAGTTAAGAGGAAAGGCCTTCTATTTTGTCAATGGCAAAGTATTCTGTG AGGAAGACTTTTTGTATTCTGGCTTTCAGCAGTCTGCGGACAGGTGTTTTCTTTGTGGACACCTGATCAT GGACATGATCCTACAGGCCCTAGGGAAGTCCTATCACCCCGGCTGTTTCCGCTGCGTCATCTGTAATGAA TGTCTGGATGGGGTTCCTTTCACCGTGGACTCTGAGAACAAGATCTACTGTGTCCGAGACTATCACAAGG TGCTGGCCCCCAAGTGTGCAGCCTGTGGCCTTCCCATCCTTCCGCCAGAGGGTTCAGACGAGACCATCAG AGTTGTGTCCATGGACAGGGACTACCACGTAGAGTGCTACCACTGTGAGGACTGTGGTCTGGAGCTCAAT GACGAGGACGGTCACCGCTGCTATCCACTGGAGGACCACCTGTTCTGTCACTCCTGCCATGTCAAGAGAC TGGAGAAAGGACCCTCACCTGCACCCCTCCACCAGCACCACTTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013860 |
| Insert Size | 2007 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013860.2, NP_038888.2 |
| RefSeq Size | 4864 bp |
| RefSeq ORF | 2007 bp |
| Locus ID | 29806 |
| UniProt ID | Q9QXD8 |
| Gene Summary | Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209904 | Limd1 (tGFP-tagged) - Mouse LIM domains containing 1 (Limd1) |
CNY 6370.00 |
|
| MR209904 | Limd1 (Myc-DDK-tagged) - Mouse LIM domains containing 1 (Limd1) |
CNY 5384.00 |
|
| MR209904L3 | Lenti ORF clone of Limd1 (Myc-DDK-tagged) - Mouse LIM domains containing 1 (Limd1) |
CNY 7700.00 |
|
| MR209904L4 | Lenti ORF clone of Limd1 (mGFP-tagged) - Mouse LIM domains containing 1 (Limd1) |
CNY 7784.00 |
