Ccdc8 (NM_001101535) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ENSMUSG00000041117
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC220481 representing NM_001101535 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCAGATTGGGGAGGATGTTGACTATCTGCTCATCCCCCGGGAGGTCCGCCTGGCTGGGGGCGTGT GGAGAGTCATCTCCAAGCCTGCAACGAAGGAAGCTGAGTTTCGGGAGCGGCTAATCCAGTTTTTGCAGGA GGAGGGCCGCACCCTGGAAGATGTGGCCCGTATCATCGAAAAGAGCACTCCACACCCACCCCAACCCCGC AAAAGAACCAAGGAGCTGAGAGTGAGGAGAGTCCCGCAGATGGTCACTCCTCCACTTCGGCTGGTTGTAG GTACCTATGACAGCAGCAATGGCAGCGACAGTGAGTTGAGTGACTTCGATACCTCCAAAGTCAAAGGCAA CAGGAGCTCCTCCGGGAGGACCAGGAAGGTGCGCAAAATGCCGGTCAGTTACTTGGGCAGCAAATTCCTG GGCAGCGATGTGGAGAGTGAGGATGATCAGGAGCTGGTGGAGGCCTTCCTCCGCCGTGGGGAGAAGCCCA GCGCGCCTCCTCCACGACGCCGAGTCAATCTTCCAGTGCCCATGTTTGAGAACAACCTGGGGCCCCAGCC ATCCAAGGCCGACAGATGGAGGGAGTATGTTAGCCAGGTGTCCTGGGGGAAGCTGAAACAGAGGGTGAAG GGCTGGGCACCCAGATCAGGCTCCGAGGTGGGCCAGACCCAGCAGGCCTCCACAGCAGCTGAGAGGGCTG GAGAAATGAGACACAGCCAAGCCAGCTCGGACGATGATAGCTCCAGAAACACAGGAGACCGGAGTGACCA GATGCTGGGTACTAGACGCTGGAAGCCCAAGATCAAGTGGGTTTCCCTGAGACGATGCAGGAAGGAGCAG GTGCCACCCTTCGCTCAGGGAACAGGCATGCCAGCTGAGGAGCACCCAGAGGCTGCAGAGAACCAGGGGG CAGAGGCTGCAGCTAACCAGAGGGCAGAGCCTCTAGCTAGTCCAAGGGCAGAGGCTGCAGCTAGTCCAAG GGCAGAGACTGCAGCTGATCCAAGAGTAGAGGCTGTTGCTAGTCCAAGAGCAGAGGCTGCAGCTAGTCCA AGGGCAGAGGCTGTAGCTGATCCAAGAGCAGAGGCTGCAGCTAGTCCAAGGGCAGAGGCTGCAGCTAGTC CAAGGACTGAGGCTGCAGCTAGTCTCAGGGCAGAGGCTGTTGCTAGTCCCAGGGCAGAGGCTGCAGCTAG TCCAAGGGCAGAGGCTGCAGCTGATCCAAGAGCAGAGGCTGCAGCTAGTCCAATAGCAGAGGCTGCTGCT AACCAGAAGGCAGAGCTTGTAGATAGTCCAAGGGCAGAGACTGCAGCTGATCCAAGAGCAGAGGCTGCAG CTAGTCCAAGGGCAGAGGCTGTAGCTGATCCAAGAGTAGAGGCTGCAGCTAGTCCAATAGCAGAGGCTGC TGCTAACCAGAAGGCAGAGCTTGTAGATAGTCCAAGGGCAGAGACTGCAGCTGATCCAAGAGCAGAGGCT GCAGCTAGTCCAAGGGCAGAGGCTGCAGCTGATCCAAGAGCAGAGGTTGCAGCTAGTCCAAGGGCAGAGG CTGCAGCTAGTCCAAGGGCAGAGGCTGAAGCTAGTCCAAGGGCAGAGGCTGCAGCTAGTCCAAAGGCAGA GGCTGAAGCTAATCTGAGGGTAGAGGCTGCAGCTTATCTCAGGGCAGGAGTCCCACCTGACCAGAGGGCA GAAGCCATAGATAGTCAGAGGGCAGAGGGCCCAGCTAACCAAAGAACTGGTGCTACAGAAAATCAGAGGG TGGAGGTCCTAGCTGACCAAAGGGCAGGGGTCCTCCATGACCAGAGGGAGGAGGCAGGACCTCAGGGTAT ACAGGAAGCCTCAGCTGGTTCGGGATCTAGGGCCCAGAAACAGGTAAAGACAGTGAGGTTCCAGACCCCA GGACGCTTCTCATGGTTTCGCATGCGCAGGAGAGTCTTCTGGCACACTCCCAGGTTGCCAACCCTGCCCA GGAGAGTCCCCAGGGCAGGTGAGGCCAGGAGCCTCCGGGTACTAAGGGCTGACATCAGAGCAGATGTGGA ACACAGAGAGCAAGAAGAACAGCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001101535
Insert Size	2058 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001101535.1, NP_001095005.1
RefSeq Size	2058 bp
RefSeq ORF	2058 bp
Locus ID	434130
UniProt ID	D3YZV8
Gene Summary	Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Required for localization of CUL7 to the centrosome.[UniProtKB/Swiss-Prot Function]

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