Ngef (NM_001111314) Mouse Untagged Clone
CAT＃: MC220792
Ngef (untagged) - Mouse neuronal guanine nucleotide exchange factor (Ngef), transcript variant 1, (10ug)
CNY 9120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | ephexin; Tims2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220792 representing NM_001111314
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCAAAAACTCTGAAGACTGGGGGAAACCTCAGAGGAAATCAGAGAGCAGCTCACGGAAAAGCA ATCATGGCCCAGCTGAGATGAGGCCTGCGTTACCTCCGGAGAACAGGGAGGCACCTGAGACTGGCGAGGA AACTCAAAACGAAGAACCCCGCCGCCTCATCCCAATCCAGAGGCATTCCCTCTTCAATCGTGCTGTGAGA CACAGGCACAAGGCCAGGAGCACGTCTGAGCGGAGAGCCAGTGACCAAGCAGATTTGCCAAAAATGGGGA AATCTGTAAATGAACGGTCGGCCTTCAATCTGCCCCAGGGGAGATTGCCTCCCTGGAGAACACCAGCCCA GAGGGACACAGGAGCGCAGGAAGCCAGCGAGTCATCCTCCACCCCTGGAAATGGAACCACACCCGAGGAG TGCCCAGCCCTCACCGACAGCCCCACCACTCTCACGGAGGCCCTGCAGATGATCCATCCCATTCCCGCCG ACTCCTGGAGAAACCTCATTGAACAAATAGGGCTCCTGTATCAAGAGTATAGAGACAAATCGACTCTCCA AGAAATTGAAACACGGAGGCAGCAGGATGCAGAAATCCAAGGCAACTCCGATGGGTCCCAGGTTGGGGAA GACGCTGGAGAGGAGGAGGAGGAGGAGGAGGAGGGAGAGGAGGAGGAGCTGGCCAGCCCTCCTGAGAGGA GAGCTCTGCCTCAGATCTGCCTGCTCAGCAACCCCCACTCCAGGTTCAACCTCTGGCAAGACCTTCCTGA GATCCAGAGCAGTGGCGTGCTGGACATTCTCCAGCCGGAGGAGATCAGGCTGCAGGAGGCCATGTTTGAG TTGGTTACCTCTGAGGCCTCCTACTATAAGAGCCTGAACCTGCTGGTGTCGCACTTCATGGAGAACGAGC GTCTGAAGAAGATCCTGCATCCATCTGAGGCCCACATCCTCTTTTCCAATGTCCTGGATGTCATGGCTGT CAGTGAGCGGTTTTTGCTGGAGCTAGAGCACCGCATGGAGGAGAACATTGTTATCTCGGATGTGTGCGAC ATCGTGTACCGTTACGCAGCTGATCACTTCTCGGTCTATATCACTTACGTCAGTAACCAGACCTACCAGG AAAGGACATACAAGCAGCTCCTACAGGAGAAGGCCGCTTTCCGGGAACTGATCGCACAGTTGGAGCTGGA CCCCAAATGCAAGGGCCTGCCTTTCTCCTCCTTCCTCATCTTGCCTTTCCAGAGGATCACGAGACTCAAG CTGCTGGTCCAGAATATCCTGAAGAGAGTGGAGGAGAGGTCTGAACGTGAAGGCACCGCCTTGGATGCCC ACAAGGAGCTAGAAATGGTGGTAAAGGCATGCAATGAGGGTGTCCGGAAGATGAGCCGCACAGAACAGAT GATCAGCATTCAGAAGAAGATGGAGTTCAAGATCAAGTCGGTACCCATCATCTCACACTCCCGGTGGCTG CTGAAGCAGGGTGAGCTGCAGCAGATGTCCGGCCCCAAGACCTCCCGCACCCTGCGGACCAAGAAGCTCT TCAGAGAAATTTACCTCTTCCTCTTCAATGACCTGCTGGTGATCTGCCGGCAGATCCCTGGAGACAAGTA CCAGGTGTTTGATTCGGCCCCAAGGGGCCTGCTTCGAGTGGAGGAGCTGGAGGACCAGGGTCAAACACTG GCTAATGTGTTCATCCTGCGGCTGCTGGAAAATGCAGATGACCGAGAGGCCACCTATATGCTGAAGGCAT CCTCCCAGAGCGAGATGAAGCGCTGGATGACCTCACTGGCCCCCAACAGGAGGACCAAGTTTGTATCCTT CACATCTCGGCTGTTGGACTGTCCCCAGGTCCAGTGTGTGCACCCGTATGTGGCCCAGCAGCCTGATGAA CTGACGCTGGAACTGGCAGATATCCTGAACATCCTGGAGAAGACAGAGGATGGGTGGATCTTTGGTGAGC GGCTGCATGACCAGGAGAGAGGCTGGTTCCCCAGTTCCATGACAGAGGAGATCCTGAACCCCAAGATCCG CTCCCAGAACCTCAAGGAATGTTTCCGGGTACATAAGATGGAAGACCCTCAGCGCAGCCAGAATAAGGAC CGCAGGAAGCTGGGCAGCCGGAATCGTCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001111314 |
| Insert Size | 2133 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001111314.1, NP_001104784.1 |
| RefSeq Size | 3127 bp |
| RefSeq ORF | 2133 bp |
| Locus ID | 53972 |
| UniProt ID | Q8CHT1 |
| Gene Summary | Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210191 | Ngef (Myc-DDK-tagged) - Mouse neuronal guanine nucleotide exchange factor (Ngef), transcript variant 1 |
CNY 5208.00 |
|
| MR210191L3 | Lenti ORF clone of Ngef (Myc-DDK-tagged) - Mouse neuronal guanine nucleotide exchange factor (Ngef), transcript variant 1 |
CNY 7700.00 |
|
| MR210191L4 | Lenti ORF clone of Ngef (mGFP-tagged) - Mouse neuronal guanine nucleotide exchange factor (Ngef), transcript variant 1 |
CNY 7700.00 |
