Ezh2 (NM_007971) Mouse Untagged Clone
CAT＃: MC221274
Ezh2 (untagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1, (10ug)
CNY 9690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Enx-1; Enx1h; KMT6; mKIAA4065 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221274 representing NM_007971
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAGACTGGGAAGAAATCTGAGAAGGGACCGGTTTGTTGGCGGAAGCGTGTAAAATCAGAGTACA TGAGACTGAGACAGCTCAAGAGGTTCAGAAGAGCTGATGAAGTAAAGACTATGTTTAGTTCCAATCGTCA GAAAATTTTGGAAAGAACTGAAACCTTAAACCAAGAGTGGAAGCAGCGGAGGATACAGCCTGTGCACATC ATGACTTCTGTGAGCTCATTGCGCGGGACTAGGGAGTGTTCAGTCACCAGTGACTTGGATTTTCCAGCAC AAGTCATCCCGTTAAAGACCCTGAATGCAGTCGCCTCGGTGCCTATAATGTACTCTTGGTCGCCCTTACA ACAGAATTTTATGGTGGAAGACGAAACTGTTTTACATAACATTCCTTATATGGGGGATGAAGTTCTGGAT CAGGATGGCACTTTCATTGAAGAACTAATAAAAAATTATGATGGAAAAGTGCATGGTGACAGAGAATGTG GATTTATAAATGATGAAATTTTTGTGGAGTTGGTAAATGCTCTTGGTCAATATAATGATGATGATGATGA CGATGATGGAGATGATCCAGATGAAAGAGAAGAAAAACAGAAAGATCTAGAGGATAATCGAGATGATAAA GAAACTTGCCCACCTCGGAAATTTCCTGCTGATAAAATATTTGAAGCCATTTCCTCAATGTTTCCAGATA AGGGCACCGCAGAAGAACTGAAAGAAAAATATAAAGAACTCACGGAGCAGCAGCTCCCAGGTGCTCTGCC TCCTGAATGTACTCCAAACATCGATGGACCAAATGCCAAATCTGTTCAGAGGGAGCAAAGCTTGCATTCA TTTCATACGCTCTTCTGTCGACGATGTTTTAAGTATGACTGCTTCCTACATCCCTTCCATGCAACACCCA ACACATATAAGAGGAAGAACACAGAAACAGCTTTGGACAACAAGCCTTGTGGACCACAGTGTTACCAGCA TCTGGAGGGAGCTAAGGAGTTTGCTGCTGCTCTTACTGCTGAGCGTATAAAGACACCACCTAAACGCCCA GGGGGCCGCAGAAGAGGAAGACTTCCGAATAACAGTAGCAGACCCAGCACCCCCACCATCAGTGTGCTGG AGTCAAAGGATACAGACAGTGACAGAGAAGCAGGGACTGAAACTGGGGGAGAGAACAATGATAAAGAAGA AGAAGAGAAAAAAGATGAGACGTCCAGCTCCTCTGAAGCAAATTCTCGGTGTCAAACACCAATAAAGATG AAGCCAAATATTGAACCTCCTGAGAATGTGGAGTGGAGTGGTGCTGAAGCCTCCATGTTTAGAGTCCTCA TTGGTACTTACTACGATAACTTTTGTGCCATTGCTAGGCTAATTGGGACCAAAACATGTAGACAGGTGTA TGAGTTTAGAGTCAAGGAGTCCAGTATCATAGCACCTGTTCCCACTGAGGATGTAGACACTCCTCCAAGA AAGAAGAAAAGGAAACATCGGTTGTGGGCTGCACACTGCAGAAAGATACAACTGAAAAAGGACGGCTCCT CTAACCATGTTTACAACTATCAACCCTGTGACCATCCACGGCAGCCTTGTGACAGTTCGTGCCCTTGTGT GATAGCACAAAATTTTTGTGAAAAGTTTTGTCAATGTAGTTCAGAGTGTCAAAACCGCTTTCCTGGATGT CGGTGCAAAGCACAATGCAACACCAAACAGTGTCCATGCTACCTGGCTGTCCGAGAGTGTGACCCTGACC TCTGTCTCACGTGTGGAGCTGCTGACCATTGGGACAGTAAAAATGTATCCTGTAAGAACTGTAGCATTCA GCGGGGCTCTAAAAAGCACTTACTGCTGGCACCGTCTGATGTGGCAGGCTGGGGCATCTTTATCAAAGAT CCTGTACAGAAAAATGAATTCATCTCAGAATACTGTGGGGAGATTATTTCTCAGGATGAAGCAGACAGAA GAGGAAAAGTGTATGACAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGC AACCCGAAAGGGCAACAAAATTCGTTTTGCTAATCATTCAGTAAATCCAAACTGCTATGCAAAAGTTATG ATGGTTAATGGTGACCACAGGATAGGCATCTTTGCTAAGAGGGCTATCCAGACTGGTGAAGAGTTGTTTT TTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTGGGCATCGAACGAGAAATGGAAATCCCTTG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007971 |
| Insert Size | 2241 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007971.2, NP_031997.2 |
| RefSeq Size | 2665 bp |
| RefSeq ORF | 2241 bp |
| Locus ID | 14056 |
| UniProt ID | Q61188 |
| Gene Summary | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). This isoform is also known as ENX-1A. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210436 | Ezh2 (tGFP-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2) |
CNY 9792.00 |
|
| MR210436 | Ezh2 (Myc-DDK-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 8192.00 |
|
| MR210436L1 | Lenti ORF clone of Ezh2 (Myc-DDK-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 7700.00 |
|
| MR210436L2 | Lenti ORF clone of Ezh2 (mGFP-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 10592.00 |
|
| MR210436L3 | Lenti ORF clone of Ezh2 (Myc-DDK-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 7700.00 |
|
| MR210436L4 | Lenti ORF clone of Ezh2 (mGFP-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 1 |
CNY 10592.00 |
