Bard1 (NM_007525) Mouse Untagged Clone
CAT＃: MC221420
Bard1 (untagged) - Mouse BRCA1 associated RING domain 1 (Bard1), (10ug)
CNY 5608.00
CNY 9880.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | ENSMUSG00000060893; ENSMUSG00000073653 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221420 representing NM_007525
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACGCCGGCCGCCGAGGGTCTGCTCTGGGAACCAGCCTGCTCCCGTGCCCGCCATGGAGCCGGCTA CCGACGGGCTTTGGGCCCACAGCCGCGCGGCGCTTGCCCGCCTGGAGAAGCTGCTGCGCTGCTCCCGCTG TGCTAATATTCTGAAGGAGCCCGTGTGCTTAGGAGGATGTGAGCACATCTTCTGTAGTGGTTGTATAAGT GACTGTGTTGGCTCAGGATGCCCAGTGTGTTACACCCCAGCCTGGATCCTAGACCTCAAGATAAACCGAC AATTGGACAGCATGATCCAGCTTTCTAGTAAGCTCCAAAATTTGCTCCATGACAATAAAGATTCAAAAGA CAACACATCTAGGGCAAGTTTATTTGGTGATGCAGAAAGGAAGAAGAATTCAATAAAAATGTGGTTTAGT CCTCGAAGTAAGAAGGTTAGATATGTTGTGACTAAAGTGTCAGTACAAACACAGCCTCAAAAGGCAAAGG ACGACAAAGCCCAGGAAGCCTCAATGTATGAATTTGTTTCCGCAACTCCCCCTGTAGCTGTTCCTAAGAG TGCTAAAACAGCTTCTAGAACATCTGCAAAAAAGCATCCAAAGAAATCTGTAGCTAAGATCAACAGGGAG GAGAATTTAAGGCCAGAAACAAAGGATAGTAGATTTGATTCCAAAGAGGAGCTGAAGGAAGAGAAGGTTG TCTCCTGTAGCCAAATACCAGTTATGGAGAGACCACGGGTAAATGGTGAAATAGACTTATTAGCAAGTGG TTCTGTTGTAGAACCTGAATGTTCTGGAAGCTTGACTGAAGTCTCTTTACCATTGGCTGAGCATATAGTG TCTCCAGACACTGTGAGCAAGAATGAAGAGACTCCGGAGAAGAAGGTCTGTGTAAAAGATCTTCGTTCAG GAGGGAGTAATGGAAATCGTAAAGGCTGCCACAGGCCTACCACTTCTACTTCTGACAGTTGTGGGAGCAA CATTCCGAGCACCAGCAGAGGCATCGGTGAGCCAGCATTGCTTGCAGAAAACGTAGTGTTGGTTGACTGC TCTTCACTGCCTTCAGGCCAGCTACAGGTTGATGTCACACTCAGGAGAAAGAGTAACGCATCAGATGACC CCCTTAGCCTTTCACCGGGCACACCCCCACCTCTGCTGAACAATTCAACTCACAGACAAATGATGTCAAG CCCCTCCACAGTGAAGCTGTCTTCTGGTATGCCAGCCAGGAAAAGAAATCACAGAGGAGAGACCTTACTT CATATTGCTTCTATTAAGGGTGATATACCTTCTGTTGAATACCTCTTGCAAAATGGAAACGACCCAAATG TTAAAGACCATGCTGGATGGACACCGTTGCATGAAGCCTGCAGTCATGGGCACCTGAAGGTAGTGGAGTT GCTGCTCCAGCATAATGCCCTGGTGAACACCCCTGGCTATCAGAATGACTCGCCACTCCACGATGCCGTC AAGAGTGGCCACATCGATATAGTCAAGGTGTTACTGTCCCACGGTGCTTCCAGGAATGCTGTTAACATAT TTGGTGTGCGGCCTGTGGACTATACAGACAATGAGAATATAAGATCATTATTGCTGCTGCCAGAGGAGAA TGAATCATTCTCAACTAGCCAGTGCTCTATCGTGAACACCGGGCAGCGAAAGAATGGGCCGCTGGTATTT ATAGGCAGTGGGCTTTCTTCACAGCAGCAGAAAATGCTCAGCAAACTTGAGACAGTTCTTAAGGCTAAAA AGTGTATGGAATTTGACAGTACAGTAACTCATGTCATTGTTCCTGATGAGGAAGCGCAGAGTACTCTGAA GTGTATGCTTGGGATTCTCAGTGGATGCTGGATCCTGAAGTTTGATTGGGTGAAAGCCTGTCTGGACAGC AAAGTACGTGAGCAGGAAGAAAAGTATGAAGTTCCTGGAGGTCCACAGAGGAGCAGGCTCAACAGAGAGC AGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTTTTTGGGGGGAAACTTCAAGCATCATCCAAGGGA TGACCTCCTTAAGCTCATTGCTGCAGCAGGAGGCAAAGTCCTGAGTCGCAAGCCCAAGCCAGACAGTGAT GTGACTCAGACCATCAACACGGTTGCATACCATGCCAAGCCTGAGTCGGATCAGCGCTTCTGTACGCAGT ACATCGTCTACGAAGACCTGTTTAACTGTCACCCAGAGAGGGTTCGGCAGGGCAAAGTCTGGATGGCTCC GTCCACCTGGCTTATCAGCTGTATAATGGCCTTTGAATTGCTTCCTCTTGACAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007525 |
| Insert Size | 2298 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC128370, AAI28371 |
| RefSeq Size | 2386 bp |
| RefSeq ORF | 2298 bp |
| Locus ID | 12021 |
| UniProt ID | O70445 |
| Gene Summary | E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217719 | Bard1 (tGFP-tagged) - Mouse BRCA1 associated RING domain 1 (Bard1), (10ug) |
CNY 6370.00 |
|
| MR217719 | Bard1 (Myc-DDK-tagged) - Mouse BRCA1 associated RING domain 1 (Bard1) |
CNY 5608.00 |
|
| MR217719L3 | Lenti ORF clone of Bard1 (Myc-DDK-tagged) - Mouse BRCA1 associated RING domain 1 (Bard1) |
CNY 7700.00 |
|
| MR217719L4 | Lenti ORF clone of Bard1 (mGFP-tagged) - Mouse BRCA1 associated RING domain 1 (Bard1) |
CNY 7700.00 |
