Ddx20 (NM_017397) Mouse Untagged Clone
CAT＃: MC221871
Ddx20 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), (10ug)
CNY 10550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | dp103; GEMIN3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221871 representing NM_017397
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGTTTGAAGTCCCGGCGGCCTTGACAACCTCCGAGTCCACTATGGCTGCGGAGCGTG CGGCCGCGCCGGTGCAGGCTGTGGAGCCGACTCCGGCATCCCCGTGGACCCAGCGGACCGCACACGACAT CGGCGGGCCGCGCACACGTACCGGGGACGTGGTGCTGGCGGAGCCCGCGGACTTCGAGTCGCTGCTGCTG TCGCGACCGGTGCTGGAGGGTCTGCGGGCGGCCGGCTTCGAGAGACCCTCTCCGGTGCAGCTCAAGGCCA TCCCGTTGGGGCGCTGCGGGCTCGATTTAATTGTCCAAGCTAAGTCGGGCACTGGAAAAACTTGTGTATT CTCCACCATTGCTCTGGACTCTCTGATTCTTGAAAACTATAGTACTCAGATTTTGATATTGGCTCCCACA AGAGAAATTGCTGTTCAGATACACTCGGTTATCACAGCCATTGGAATAAAAATGGAAGGATTAGAATGTC ATGTCTTTATTGGAGGGACTCCATTATCACAAGACAAAACCAGACTTAAAAAGTGTCATATTGCTGTTGG ATCTCCTGGTAGAATTAAACAGCTCATAGAACTTGACTACCTGAATCCGGGCAGTATCCGTCTCTTTATT CTTGATGAAGCAGATAAGCTTTTAGAAGAAGGCAGTTTCCAGGAGCAAATAAATTGGATTTACTCCTCTT TACCTGCGAGTAAGCAGATGTTGGCAGTATCTGCTACTTACCCTGAAGTTTTGGCTAATGCTTTGACAAG GTATATGAGAGATCCCACTTTTGTAAGATTGAATCCCAGCGATCCAAGTCTCATAGGTTTGAAGCAGTAC TACCAAGTTGTCAATTCTTACCCTTTGGCTCATAAGATTTTTGAGGAAAAGACTCAGCACTTACAGGAAC TTTTTAGTAAAGTCCCATTTAATCAAGCCTTGGTGTTTTCTAATTTGCACAGCAGAGCTCAACATTTGGC TGATATCCTTTCTTCTAAAGGTTTTCCTACTGAATGCATTTCAGGTAACATGAATCAGAATCAGCGCCTT GATGCTATGGCTAAACTGAAGCAGTTTCATTGCAGAGTCCTCATTTCTACAGACTTGACGTCCCGTGGGA TTGACGCAGAGAAGGTGAATCTAGTTGTAAATCTCGATGTGCCGTTAGACTGGGAGACTTACATGCATCG GATTGGCAGAGCTGGCCGTTTTGGTACATTGGGACTGACAGTGACCTACTGTTGTAGAGGAGAAGAAGAA AATATGATGATGAAAATTGCTCAGAAATGTAATATCAACCTTCTTCCTTTACCAGATCCTATTCCTCCTG GCCTGATGGAAGAATGCTTGAATTGGGATGTGGAAGTTAAAGCTGCCATGCATACTTACAGCTCACCAAC TGTAGCTACTCAGTCCCCCAAAAAGCAAGTTCAAAAACTAGAGAGAGCCTTCCAGTCTCAAAGAACGCCT GGGAACCAAACGCCTTCCCCTAGAAATACTTCTGCATCTGCACTGTCAGCCAGGCCAAAGCACAGCAAAC CAAAGCTTCCTGTGAAAAGCCACTCAGAGTGTGGAGTCCTAGAAAAGGCAGCTCCACCACAAGAGTCGGG CTGTCCCGCACAGCTGGAAGAGCAAGTTAAGAATTCTGTTCAGACCTCTGTTGAGGATTCCTCCAGTAAC AGCCAGCATCAGGCCAAAGATAGCTCACCTGGGTCACTCCCTAAAATCCCTTGTCTGTCTTCTTTTAAAG TCCATCAGCCGTCTACTTTGACTTTTGCAGAACTGGTAGACGATTATGAGCACTATATTAAAGAGGGGTT AGAGAAACCTGTGGAAATTATCCGGCACTACACAGGGCCTGAGGCCCAGACCGGGAATCCTCAGAATGGC TTTGTGAGAAATAGAGTTTCTGAAGATAGAGCACAGATGTTGGTGAGTAGTAGCCAGTCTGGAGACTCAG AGAGTGACAGCGATTCTTGCAGCTCAAGGACTTCTTCCCAGAGCAAAGGTAATAAGTCTTACTTGGAAGG CTCCTCAGACACTCAGTTGAAAGACACTGAATGTACTCCTGTGGGTGGCCCTCTCTCTCTGGAACAAGTT CAGAATGGAAATGATACCCCGACTCAAGTGGAGTATCAAGAAGCCCCTGAAACCCAGGTAAAGGCAAGGC ATAAAGAAGGCGCTAACCAAAGGTCTAAGCAAAGTCGGAGAAACCCAGCACGGCGTTCTTCTTACCGAGT ACAGTCAGAACCCCAGGAAGAGAGTTGGTATGACTGTCACAGGGAAACAACTGCAAGTTTTTCTGATACT TACCAGGATTATGAGGAGTACTGGAGAGCGTATTACAGGGCATGGCAAGAGTATTATGCTGCTGCTTCCC ATTCATACTATTGGAATGCTCAGAGACATCCAAGCTGGATGGCTGCCTATCATATGAATACAGTTTATCT ACAAGAGATGATGCGTGGTAACCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017397 |
| Insert Size | 2478 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017397.3, NP_059093.3 |
| RefSeq Size | 2710 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 53975 |
| UniProt ID | Q9JJY4 |
| Gene Summary | The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226421 | Ddx20 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20), (10ug) |
CNY 6370.00 |
|
| MR226421 | Ddx20 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20) |
CNY 6040.00 |
|
| MR226421L3 | Lenti ORF clone of Ddx20 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20) |
CNY 7700.00 |
|
| MR226421L4 | Lenti ORF clone of Ddx20 (mGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (Ddx20) |
CNY 7700.00 |
