Ttll8 (NM_172818) Mouse Untagged Clone
CAT＃: MC221968
Ttll8 (untagged) - Mouse tubulin tyrosine ligase-like family, member 8 (Ttll8), (10ug)
CNY 10640.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700019P01Rik; 4933401B17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221968 representing NM_172818
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTGTCCCCCGACCCCAAACCCTCCTTTCAGGCCTCCCTCCCACACCCGGGTCCTCCGAACCCCAC CCTTGCCTCCCTGGGTATGTCTGAACTCTAAATCTTTGTCAACAGGGGTTGGGGGCCAAAAGAACCAGCT ACGAGAGGCGAGCATGGAGAACGGCGAGAGGAAGAAGCTGTCTTCCACTTTGTCAGATGGAGATCACAAA GAAGAAAATAAGTTAAAACAAGGAATTCCCCAAGATTTGTCTTCTTCCCCCAAATTAGACAGATACAAAA TAGCAAGACAGTTAACAGAAAAAGCCATCAAGGAGAGGAAGATTTTCTCCATCTATGGACACTACCCTGT CATCCGGGCCACCTTGCGAAGAAAGGGCTGGGTGGAGAAGAAGTTCAATTTCTTCCCCAAAGCTCTCCAG AACCTTGGAAGTGAAGATAAAAGTGCTGAGACCAAAGAAAATCAAGAAATAGCCTTGGAGAGATTCGATG ATATCCACGATGTGATGTCCAGGTTGGTAAAGAACGAGATCCCATACCTCCTCTGGACTATCAAGAGGGA TGTTGTGGACTACCACAGCCTGACCTGTGACCAGATGCTAAATCACTACGGGAAGACAGCATCATTCACA ACCAAGATTGGACTCTGCCTGAACATGAGAAGTCTGCCTTGGTACGTCCAAGCCAACCCGAACACCTTCT TCCCACGGTGCTATGGCCTTTGCACTGAGAGCGAGAAGCAGGAATTCTTGGATGACTTCCGGCGCACGGT GGCTGCCAGCATCCTCAAGTGGGTAGTCCTTCACCAGAATTACTGCAGCAAAGTCAAGGGCAAGAGTAAG AAGGAGGAGGCCAAGAACAGTGACCCCAGCCCCAAGAAAGATCCTGAAAATCCTGACCTCAAGCTACCAA GCCTCTCGGGACAGGTTGTAGACACAGCTTGCAAGGTGTGCCAGGCCTACCTGGGACAGCTAGAGCATGA AGACATAGACGTATCGGAGGCCAGCACCGAGGCCTTGTCTGAGGAGGAATGGAATGACCTGACACAGCAG TACTATCTACTGGTTCATGGCAATGCTTCCATCACTGATTCGAAAAGTTACTTTGCGCAATGCCAAGCTC TGCTGAGTAAGATCAGTTCTGTGAACCCTCAGACAGAGATCGATGGGATCCGGAACATCTGGATCATAAA GCCTGCAGCCAAGTCCCGGGGTCGAGATATTGTGTGTATGGACCGTGTGGAGAACATCCTGAGTCTGGTG GCCGCAGACAGCCAGACCACAAAGGACAACAAATGGGTGGTCCAGAAGTACATCGAGACCCCAATGCTCA TCTACGACACCAAGTTTGACATCAGACAGTGGTTCCTCGTCACAGACTGGAATCCCCTAACCATCTGGTT CTACAAAGAAAGCTACCTCCGCTTCTCCACACAGCGCTTCTCCCTGGACAAGCTGGACAGCGCCATCCAC CTGTGTAACAACTCCATCCAGAGGCGCCTCAAAAATGATAAAGAGCGCAGTCCGCTGCTACCTTGTCATA ACATGTGGACCAGCACTCGTTTCCAGGAGTACCTGCAGAAGAGGGGCCGAGGAGGAACGTGGGGTAGCAT CATCTACCCGTCTATGAAAAGAGCTGTCACCAATGCCATGAGGGTAGCCCAGGACCACGTAGAAGCCCGT AAGAACAGCTTCGAGCTCTACGGAGCCGACTTCATCCTGGGGCGAGACTTCAAGCCATGGCTTATCGAGA TCAACTCCAGCCCCACCATGCACCCCTCCACTCCCGTCACAGCTCAGCTCTGTGCCCAGGTGCAGGAGGA CACCATCAAGGTGGTGGTGGATCGCAAACTGGACCGAAACTGTGACATCGGCAACTTTGAGCTTCTGTGG CGGCAGCCTGCCGTGGAGCTGCCACCGTTCAACGGGTCTGACCTCTGTGTGGAAGGTATCAGCGTGAAGA AAGCCAAGAAGCAAATGCCGCCCATTGCCAGCGTCGGCCTCTCAGAATCACTCTTAGATGCTCCACCCAA AGTGCGGAGTGCCCGGGCCCTGATGGAAACAGTGATCAGGCCTCCAAGAACGACCGTGCGGCAGGACTGG AAACGGGAAGAAGCAAAGGTACTTTCAACCACTTGGTCCATGCCAGTAATGGATGCAGAGGTCAGAGGTC GAGCAAAGCCCATCTATGCATTCGAGGTCAATGACTACCAACATGTAGACAATAAGTCCCACAAGAGCGG TTATACCAGAGTCCAATCCAGTAAAGTCCCAGGGGTAACCTTAACAAGTGCACAGCACCCTGCCCTGTTT GCACAGACTATGAAACCCACACAGATGACCTCGTCTCCACCACCCACTGCGTCAGGTAACCATAGAGACT CTTCGCCTTTCTGTCCCATTGTATTTGAGGAACTCTGGCTCCACCCCAATTCTCAGAGACGACCTTCAAG CTGTATTCTCCAAAGCAGGGCACAGGGCTGGATCAGGGGGATCCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172818 |
| Insert Size | 2499 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172818.3, NP_766406.2 |
| RefSeq Size | 2976 bp |
| RefSeq ORF | 2499 bp |
| Locus ID | 239591 |
| UniProt ID | A4Q9F1 |
| Gene Summary | Monoglycylase which modifies both tubulin and non-tubulin proteins, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues of target proteins. Monoglycylates tubulin, with a preference for alpha-tubulin toward beta-tubulin. Has the ability to modify non-tubulin proteins such as ANP32A, ANP32B, SET and NCL. Involved in the side-chain initiation step of the glycylation reaction by adding a single glycine chain to generate monoglycine side chains. Not involved in elongation step of the polyglycylation reaction.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). CCDS Note: The coding region has been updated to extend the N-terminus to an upstream in-frame start codon. The uAUG appears to be restricted to rat and mouse, and has a weak Kozak signal compared to a strong one for the better conserved downstream AUG, which raises the possibility of the dAUG being use due to leaky scanning. The update changes the protein length from 781 aa to 832 aa, but the splice sites are unaffected. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219988 | Ttll8 (tGFP-tagged) - Mouse tubulin tyrosine ligase-like family member 8 (Ttll8), (10ug) |
CNY 6370.00 |
|
| MR219988 | Ttll8 (Myc-DDK-tagged) - Mouse tubulin tyrosine ligase-like family, member 8 (Ttll8) |
CNY 6096.00 |
|
| MR219988L3 | Lenti ORF clone of Ttll8 (Myc-DDK-tagged) - Mouse tubulin tyrosine ligase-like family, member 8 (Ttll8) |
CNY 7700.00 |
|
| MR219988L4 | Lenti ORF clone of Ttll8 (mGFP-tagged) - Mouse tubulin tyrosine ligase-like family, member 8 (Ttll8) |
CNY 7700.00 |
