Mastl (NM_025979) Mouse Untagged Clone
CAT＃: MC222270
Mastl (untagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl), (10ug)
CNY 11120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2700091H24Rik; C88295; GW; GWL; MAST-L; THC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222270 representing NM_025979
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCGCCTCGGCAAGTGAGGAGAATGAAGGAGGTGCGGCGATAGAGGAGTGCGTGAGTAGGATCC CGGTACCAAGACCACCCTCCATTGAGGAGTTCACCATAGTGAAGCCCATTAGCCGTGGTGCATTCGGGAA AGTGTACCTGGGACAGAAAGGCGGCAAGTTGTACGCAGTGAAGGTTGTCAAAAAGGCAGACATGATCAAT AAAAATATGACTCATCAGGTACAAGCTGAGAGAGATGCCCTGGCACTGAGCAAAAGCCCTTTTGTTGTGC ATCTCTATTACTCACTGCAGTCAGCAAGCAACATCTACTTGATAATGGAGTATCTTATTGGTGGAGATGT CAAGTCTCTCCTACATATATATGGTTATTTTGATGAAGAGATGGCTATAAAATATATTTCTGAAGTAGCA TTGGCTTTAGACTACCTGCACAGACATGGAATCATCCATAGGGATTTGAAACCGGACAATATGCTTATTT CCAATGAGGGTCATATTAAACTGACAGATTTTGGCCTTTCCAAAGTTACTTTGAATAGAGACATCAATAT GATGGATATTCTCACAACACCATCAATGTCTAAACCTAAGCAAGATTATTCAAGAACTCCAGGACAAGTC TTATCTCTCATCAGTTCTTTGGGATTTTTCACACCAGTTGGAGAAAAAGATCAAGACTCTGCAAACATGT TTTCAGCCCCTAAGTCTGCTGCACAACTTTCTCGAGGATTCATTTGTCCTATGTCTGTAGATCAGAAGGA GCCGACTTCTTATTCGAGCAAACTGCTAAAATCATGTTTTGAAACCCTTTCATCCAATCCAGAAATACCT GTGAAATGTCTGACTTCTAATCTACTCCAGTGTAGGAAAAGACTGGGTACCTCAAGTACTAGTAGCCAGT CTCATACCTTCGTATCCAGTGTGGAGTCAGAATGCCACAGCAATCCCAAATGGGAAAGAGACTGCCAGAG TACCGAATCATCAGGCTGTGCAATGAGCTGGAATGCAGTTGAAATGCTGTATGCAAAATCTACAAGTGCC ATCAAGACAAAAACAGAACTGGAATTAGCACTTTCTCCCATTCATGACAGCAGTGCCATTCCTGCTGCTG GAAGCAACCAGGTGACCCTCCCTAGAAAATGCTTCAGGGAAATCTCTTGGGAAGCAAGGGACCCAGATAA TGAGAACATGACCATTGACAAGGGACAGTCTGGTTTCTGTCAGTCCAGTCAGAGGTCTGTAAATTCTAGT GCTACATCTGAAGAACACCTTGGTAAAAGAAATTATAAAAGAAATTTCCACTTGGTTGACTCCAGTCCTT GTCAGGAAATTATGCAAAGTAAAAAAAACTGTACAGAGTATGAGGCCAATAAGGAAAGGCAAGGCTGCCG TGCAAATCAAAGTACAGGCTTGACAACCGAAGTCCAGAACCTGAAGCTATCAGGGTGTGAAAGTCAGCAA CTTGACTATGCCAATAAGGAGAACATTGTCACCTATTTAACTGACAGACAAACCCCAGAAAAACTGCATA TACCAACGATAGCAAAGAACCTTATGTCTGAACTAGATGAAGACTGTGAGCTGAGCAGTAAAAAGGACTG CCTCAGTTCTAATTCTGTATGTTCTGATGAGGATAGAGCTCTTAAAACTACCTGTGTGGATTCGGATTCA TCTTTTCCTGGAGTGTCTATGATGGAGAGTTCACTGGAAATTCAGGCCTTAGAACCAGATAAAAGCATCA GAGACTACTCTTTTGAAGAACCAAACACTGAAGATCTATTTGTGTTACCAAAATGCCAAGAAAATTCCTT GCCACAAGATGACTGCCATGCTTGTATCCAAGACAGTAGCCAAGTGTCAGCTCATCCCTCAAAGGCGCCC AAAGCATTGACCTCTAAAATCAACGTCGTGGCTTTTCGAAGTTTTAACAGTCATATTAACGCATCTACTA ATTCGGAACCATCCAAAATCAGCATAACTTCTTTAGATGCTATGGACATTTCCTGTGATTACAGTGGTTC ATACCCCATGGCTGTGAGCCCTACTGAGAAAGGAAGGCACTACACGTCACATCAGACTCCAAATCAGGTC AAGTTGGGAACATCATACAGAACTCCAAAGAGTGTGAGAAGAGGGGCAGCCCCAGTAGATGACGGGCGAA TTCTAGGGACTCCAGATTACCTGGCACCTGAGCTCTTACTGGGTACAGCCCATGGTCCTGCAGTAGACTG GTGGGCTCTTGGAGTTTGCTTGTTCGAATTTCTGACAGGAATTCCCCCTTTCAATGATGAAACACCACAA CAAGTATTCCAGAATATTTTAAAAAGAGATATCCCATGGCCAGAAGGCGAAGAAAAGCTATCTGATAATG CTCAAAGTGCAATGGACATGCTTTTAACCATTGATGATTCAAAGAGAGCTGGAATGAGAGAACTAAAACA GCATCCTCTCTTCAGTGAAGTGGACTGGGAAAATCTGCAGCATCAGACTATGCCTTTCGTACCCCAACCA GACGACGAAACAGATACATCCTATTTTGAAGCCAGAAATAATGCTCAACATCTGACCATATCTGGGTTTA GTCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_025979 |
| Insert Size | 2598 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025979.4, NP_080255.3 |
| RefSeq Size | 4652 bp |
| RefSeq ORF | 2598 bp |
| Locus ID | 67121 |
| UniProt ID | Q8C0P0 |
| Gene Summary | Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211003 | Mastl (tGFP-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl) |
CNY 8360.00 |
|
| MR211003 | Mastl (Myc-DDK-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl) |
CNY 6336.00 |
|
| MR211003L3 | Lenti ORF clone of Mastl (Myc-DDK-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl) |
CNY 9500.00 |
|
| MR211003L4 | Lenti ORF clone of Mastl (mGFP-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl) |
CNY 9500.00 |
