Rnf40 (NM_172281) Mouse Untagged Clone
CAT＃: MC223131
Rnf40 (untagged) - Mouse ring finger protein 40 (Rnf40), (10ug)
CNY 12920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI848422; BRE1-B; mKIAA0661 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223131 representing NM_172281
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCCTCAGCAACAAGCGCGCCGCCGGCGACGGGGGCTCAGGACCCCCAGAGAAGAAGATGAACC GCGAGGAGAAGACCACGACCACTCTAATCGAACCCATTCGTCTCGGCGGCATTTCTTCCACGGAGGAGAT GGATTCCAAGGTACTGCAGTTCAAGAACAAGAAGCTGGCGGAGCGGTTGGAGCAGCGGCAAGCCTGCGAG GATGAGCTCCGGGAGAGAATTGAGAAATTGGAGAAGAGGCAGGCCACTGATGATGCCACACTCCTCATCG TCAACCGCTACTGGGCCCAGCTGGATGAAACTGTAGAAGCCCTCCTCCAGTGCTACGAGAACCAGAGGGA ACTGTCTTCAGGGACAGAGGTGCCTGGGTGCCAAGAGGGCCTGACCCGTGATGTGATCCCTCGACCAGAC CCAGGGACATCAGATCTGAGGGAACCGCTGCCAGTGCAGTTTCGAGCCCCTCTCAGTGAGCCGGCCTTGG CTTTTGTGGTGGCACTGGGCGCCAGCAGCTGTGAGGAAGTGGAGCTGCAGCTGCAGGGTCGGATGGAATT CTCCAAGGCTGCCGTGTCTCGGGTGGTCGAGGCCTCGGACCGCCTGCAACGGCAGGTGGAAGAACTCTGT CAGCGAGTATACAGCCGGGGGGACAGTGAGGCCCCTGGGGAGGTGGCACGGGTGCGCACCCGGGAGCTAG GCAGAGAGAACCGTAGGCTACAGGACTTGGCCACTCAGCTGCAAGAGAAACACCACCGCATCTCGTTGGA GTACTCTGAGCTCCAAGATAAAGTGACATCGACAGAGACCAAGGTCCTAGAGATGGAGACCACAGTGGAG GACCTGCAGTGGGACATTGAGAAGCTGCGCAAGCGAGAACAGAAGCTCAATAAGCACTTGGCAGAGGCCT TGGAGCAGCTCAACTCCGGCTACTATGTGTCTGGGAGCTCCACAGGCTTTCAGGGAGGCCAGATTACACT CAGCATGCAGAAGTTTGAGATGTTGAATGCAGAGCTGGAAGAAAATCAGGAATTGGCCAATAGCCGAATG GCAGAGCTGGAGAAGCTGCAGGCGGAGCTTCAGGGAGCCGTTCGGACCAATGAGCGTCTCAAGGTGGCCC TGCGTAGCCTTCCTGAGGAGGTCGTTCGTGAGACAGGGGAGTACCGCATGCTGCAGGCACAGTTCTCCCT GCTCTACAATGAGTCTTTGCAAGTGAAGACCCAGCTCGATGAGGCCCGGGGGTTGCTGCTGGCCTCCAAG AACTCCCACTTGAGACACATTGAACATATGGAGAGTGATGAATTGGGGCTGCAGAAGAAGCTGCGCACAG AAGTTATCCAGCTGGAAGACACCCTGGCCCAGGTACGCAAGGAGTACGAGATGCTGCGCATCGAGTTTGA ACAGAACCTTGCGGCCAACGAGCAGGCTGGGCCTATCAACCGGGAAATGCGCCACCTGATTAGCAGCCTC CAGAACCACAATCACCAGCTAAAAGGAGATGCCCAGCGATACAAGCGGAAACTTCGAGAAGTACAGGCTG AGATTGGTAAACTCCGGGCACAGGCCAGCGGCTCTTCCCACTGCATTCCCACCCTGAGCCACCCAGATGA CCCTGGCCTCAATGCCCTTGCCCCAGGGAAGGAAGATAGTGGGCCAGGCCCTGGAGGTACCCCTGACTGC AAGAAGGAGATGGCTTTATTGGCTGGAGCCACCTCTGCTACTTCTTCCATAAAGAAAGAGGAGCTGGTCT CCTCTGAAGATGACGCCCAGGCCCTAACCCCTGTAACCCAGGGCTTGCCCTCCAGGGGTCGAGAACCTGA GGCCAGACCCAAGCGAGAACTTCGGGAACGGGAAGGGCCTAGCCTAGGACCCCCACCTGCAGCCTCAACT CTGTCAAGGGCTGATCGAGAAAAGGCCAAGGTGGAAGAAGCCAAAAGGAAGGAATCGGAACTGCTCAAAG GTCTCCGAGCAGAGCTCAAGAAGGCTCAGGAAAGCCAGAAGGAGATGAAGCTGCTCCTGGACATGTACAA GTCAGCACCCAAGGAGCAGCGGGACAAGGTGCAGCTCATGGCAGCTGAGCGCAAGGCCAAGGCTGAGGTG GATGAGCTTCGGAGCCGCATCCGAGAGCTGGAAGAACGAGACCGAAGAGAAAGCAAGAAGATTGCGGATG AAGATGCCCTGCGGCGGATCCGGCAGGCAGAAGAGCAGATTGAACACTTGCAACGCAAATTGGGTGCCAC CAAGCAGGAGGAGGAGGCGTTGCTGTCGGAGATGGATGTAACAGGTCAGGCTTTTGAAGACATGCAGGAG CAAAACGGGCGGCTACTTCAGCAGTTACGGGAAAAGGATGACGCCAACTTCAAGCTCATGTCGGAGCGGA TCAAGGCCAACCAGATTCACAAGCTGCTCCGAGAGGAGAAGGATGAGTTGGGCGAGCAGGTTCTTGGCCT TAAGTCCCAGGTGGATGCCCAGCTGCTGACCGTACAGAAGCTTGAGGAGAAGGAGCGGGCTCTGCAGGGC AGCCTCGGGGGTGTGGAAAAGGAGTTGACTCTGCGCAGCCAGGCTCTGGAGCTTAATAAGAGAAAGGCTG TAGAAGCAGCCCAGTTGGCTGAGGACCTGAAGGTGCAGTTGGAGCATGTGCAGACTCGACTTCGAGAGAT CCAGCCCTGCCTGGCAGAGAGCCGGGCTGCCCGGGAGAAGGAGAGCTTCAACCTCAAGAGGGCTCAGGAG GACATTTCCCGGCTTCGGCGAAAACTAGAGAAACAGCGGAAAGTGGAGGTTTATGCAGATGCTGACGAGA TCCTGCAGGAGGAGATCAAGGAGTACAAGGCCCGGTTGACCTGCCCCTGCTGTAACACCCGCAAGAAGGA TGCCGTGCTTACCAAGTGCTTCCACGTTTTCTGCTTTGAATGTGTGCGGGGCCGCTATGAGGCCCGCCAG AGGAAATGTCCCAAGTGCAACGCAGCCTTTGGTGCCCACGACTTCCACCGTGTGTACATCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_172281 |
| Insert Size | 3006 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172281.2, NP_758485.2 |
| RefSeq Size | 4172 bp |
| RefSeq ORF | 3006 bp |
| Locus ID | 233900 |
| UniProt ID | Q3U319 |
| Gene Summary | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211421 | Rnf40 (tGFP-tagged) - Mouse ring finger protein 40 (Rnf40) |
CNY 9600.00 |
|
| MR211421 | Rnf40 (Myc-DDK-tagged) - Mouse ring finger protein 40 (Rnf40) |
CNY 6968.00 |
|
| MR211421L3 | Lenti ORF clone of Rnf40 (Myc-DDK-tagged) - Mouse ring finger protein 40 (Rnf40) |
CNY 10640.00 |
|
| MR211421L4 | Lenti ORF clone of Rnf40 (mGFP-tagged) - Mouse ring finger protein 40 (Rnf40) |
CNY 10640.00 |
