Dgkd (NM_177646) Mouse Untagged Clone
CAT＃: MC224002
Dgkd (untagged) - Mouse diacylglycerol kinase, delta (Dgkd), (10ug)
CNY 15680.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI841987; D330025K09; dgkd-2; DGKdelta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224002 representing NM_177646
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGGCGCCCCTCCGCCGGGTCCTCCTCAGCCACCTCCGCCGCCGCCGCCTGAGGAGT CGTCCGACAGTGAACCCGAGGCTGAGCCGGGCTCCCCGCAGAAGCTTATCCGTAAGGTGTCCACGTCGGG CCAGATCCGGCAGAAGACCATCCTCAAAGAAGGCATGCTGACCAAACAGAACAACTCGTTCCAGCGATCC AAGAGGAGGTACTTTAAGCTTCGAGGGCGAACACTTTACTATGCTAAAACTGCAAAGTCGATTATATTTG ATGAGGTAGACCTGACTGATGCCAGCGTAGCAGAATCCAGCACCAAAAATGTCAACAACAGTTTTACGGT CATCACTCCATGTAGGAAGCTCATCTTGTGTGCCGACAACAGGAAAGAAATGGAAGATTGGATTGCAGCA CTCAAGACTGTCCAGAACAAAGAGCATTTTGAGCCTACCCAATATAGCATGGACCATTTCTCAGGGATGC ACAACTGGTATGCTTGTTCCCATGCACGGCCAACCTATTGCAATGTGTGTCGTGAAGTTTTGTCAGGGGT CACATCCCATGGGCTATCCTGTGAAGTGTGTAAGTTTAAGGCTCACAAACGTTGTGCTGTGCGTGCAACT AGTAACTGCAAGTGGACCACATTGGCCTCAATCGGGAAGGATATCATTGAGGACGAGGATGGGATTGCCA TGCCTCACCAGTGGTTGGAAGGAAACCTGCCTGTGAGTGCCAAGTGTATCGTGTGTGACAAGACTTGTGG CAGCGTGTTACGCCTGCAGGACTGGCGCTGCCTCTGGTGCAAAGCCATGGTTCACACATCTTGCAAAGAG TCCTTGGTGATGAAGTGTCCACTTGGCCTGTGCAAGGTGTCTGTCATCCCTCCCACGGCACTCAACAGTA TTGACTCCGATGGGTTCTGGAAGGCCACCTGTCCCCCGTCTTGTACAAGCCCTCTGCTGGTTTTTGTCAA CTCAAAAAGTGGAGACAATCAAGGTGTGAAGTTTCTCAGAAGATTCAAGCAGCTGCTGAACCCTGCCCAG GTTTTTGACCTCATGAATGGAGGACCACACCTTGGCTTACGGTTATTTCAGAAGTTTGACACCTTCCGGA TTCTGGTTTGTGGCGGGGATGGCAGTGTTGGCTGGGTCCTCTCTGAAATCGATAGCCTCAACCTTCACAA ACAGTGTCAGCTAGGTGTGCTGCCCCTGGGGACAGGGAACGACCTGGCCCGTGTTCTTGGCTGGGGCTCT GCCTGTGATGATGATACACAGCTTCCTCAGATCTTGGCAAAGCTGGAGAGGGCCAGCACTAAGATGCTGG ACAGGTGGAGTGTCATGGCATATGAGACCAAGCTCCCTCGGCAGGCCTCCTCCTCCACTGTCACTGAGGA CTTCAGCGAGGACTCAGAGGTGCAGCAAATTCTCTTCTATGAAGACTCAGTCGCAGCTCATCTTTCTAAA ATCTTAACTTCGGACCAGCATTCTGTGGTGATCTCATCAGCCAAAGTGCTCTGTGAGACAGTGAAGGACT TTGTGGCTCGAGTGGGCAAGGCCTATGAGAAGACGACAGAGAGCTCGCAGGAGTCAGAGGTCATGGCCAA GAAGTGCTCTGTCTTGAAAGAGAAACTGGACTCTCTCCTCAAGACCTTGGATGACGAGTCTCAGGCTTCA TCCTCTCTGTCTAACCCACCTCCTACCATTGCTGAGGAGGCTGAGGATGGGGACGGATCAGGCAACATCT GTAGCTCTACTGGGGACCATTTGGTGGGCTCGGCATGCCCCTCCCGGCCACAGATCTTCCGGCCCCGGGA GCAGCTAATGCTGAGAGCCAACAGCCTGAAGAAAGCAATTCGCCAGATTATAGAGCACACAGAAAAAGCG GTGGATGAGCAGAATGCCCAGACCCAGGAGCAGCAGGGTTTTGTCCTAGGCCTCTCTGAATCTGAGAAGA AGGACTTGAAGACTGACAACAGGGTGTGCACATCATCTGTCCATAGTGAGAGCTGTGTGATTGCCAAGGG GAGAAGTCAACGCAAAGCATCCAGGGCCCCTTGTGAGAAGCTGGTGAGCAAAGGCCTGTCACTCGGCAGC TCTGCCTCACTCCCTCCTGGTACAGGAAGCCGGGACAGCCTGCCTGCTCTGAACACCAAGATCCTATATC CAAGTGTTCGGGCAGGGATGTCTGGCTCCTTGCCAGGGGGCTCAGTCATCAGTCGCTTGTTAATTAATGC TGATCCCTTTAATGCTGAACCTGAAAACCTAGAATACTACACAGAGAAGTGTGTCATGAACAACTATTTT GGCATTGGCTTGGATGCAAAGATATCTCTGGACTTCAACAACAAGCGTGATGAGCACCCAGAGAAATGCA GGAGTAGGACTAAGAACATGATGTGGTATGGTGTCCTTGGCACCAAAGAGCTGCTGCACAGAACCTACAG GAACCTGGAACAAAAGGTCCTGCTGGAGTGTGATGGCCGCCCTATTCCCCTCCCGAGTCTTCAAGGAATT GCTGTCCTCAACATTCCCAGCTATGCTGGAGGGACCAACTTCTGGGGGGGCACCAAGGAAGATGATACTT TTGCAGCTCCATCATTCGATGATAAGATTCTGGAGGTGGTTGCTGTGTTCGGCAGCATGCAGATGGCTGT GTCTCGTGTAATTAAGCTACAGCATCATCGGATTGCCCAGTGTCGCACAGTGAAGATCTCCATCCTGGGG GATGAAGGCGTGCCTGTGCAGGTGGATGGGGAAGCCTGGATCCAGCCACCTGGGTACATTCGGATCGTGC ACAAGAACAGGGCGCAGACATTGACCAGAGACAGGGCCTTTGAAAACACCCTGAAGTCTTGGGAAGACAA ACAGAAGTGTGAGCTGTCCCGCCCACCCTCCTTTTCCCTGCACCCTGAGATCCTGTCTGAGGAGGAAGCT ACCCAGATGGATCAGTTTGGGCAGGCAGCAGGGGGCCTCATTCACAGCATTCGGGAGATAGCACAGTCCC ACAGAGCCATGGAACAGGAGCTCGCACATGCTGTCAATGCCAGCTCCAAAGCCATGGAGCGAGTATACGG CAAGCCCAGAACTGCAGAGGGGTTGAACTGTAGCTTTGTTCTGGAGATGGTGAATAACATCAGAGCACTG CGCAGTGAGACAGAGCTGCTGCTGGCTGGGAAGATGGCCCTGCAATTGGATCCCCCTCAGAAGGAACGGC TCGGGGCTGCCCTAATTGAGATGGACCAGCAGCTCAGGAAGCTGACAGACACTCCCTGGCTTTGCCAGCC CTTGGAGCCTGGTGAGGAAGAGTCTCTCCAACAGAATGTGATGCTGGATCTTACTAAACGCAGCCGTAGT GGTAAATTCCGCCTTGTGACCAAGTTTAAAAAGGAGAAAAACAATAAGAACAAAGAAGTTCACAGTAACC TAGGAGGCCCTGTTCACCTCTGGGGGACAGAGGAGGTTGCTGCCTGGCTGGAGCACCTCAGTCTCTGTGA GTATAAGGACATCTTCACGCGGCACGACATCCGGGGCTCTGAGCTCCTGCACCTGGAGCGGAGGGACCTC AAGGACCTTGGTGTGACCAAAGTGGGCCATATGAAGAGGATCCTTTGTGGGATCAAGGAGCTGAGCCGGA GCTCCCCTGCCGCCGAGGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_177646 |
| Insert Size | 3663 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177646.3, NP_808314.2 |
| RefSeq Size | 5694 bp |
| RefSeq ORF | 3663 bp |
| Locus ID | 227333 |
| UniProt ID | E9PUQ8 |
| Gene Summary | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:17021016). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:17021016). By controlling the levels of diacylglycerol, regulates for instance the PKC and EGF receptor signaling pathways and plays a crucial role during development (PubMed:17021016). May also regulate clathrin-dependent endocytosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG215309 | Dgkd (tGFP-tagged) - Mouse diacylglycerol kinase delta (Dgkd), (10ug) |
CNY 11780.00 |
|
| MR215309 | Dgkd (Myc-DDK-tagged) - Mouse diacylglycerol kinase, delta (Dgkd) |
CNY 8496.00 |
|
| MR215309L3 | Lenti ORF clone of Dgkd (Myc-DDK-tagged) - Mouse diacylglycerol kinase, delta (Dgkd) |
CNY 12640.00 |
|
| MR215309L4 | Lenti ORF clone of Dgkd (mGFP-tagged) - Mouse diacylglycerol kinase, delta (Dgkd) |
CNY 12640.00 |
