Usp47 (NM_133758) Mouse Untagged Clone
CAT＃: MC224327
Usp47 (untagged) - Mouse ubiquitin specific peptidase 47 (Usp47), transcript variant 2, (10ug)
CNY 17390.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4930502N04Rik; A630020C16Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224327 representing NM_133758
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAGGAGATAGAAAATGCTGCTGAAGAACCTAGAGTCT TATGTATAATACAAGACACTACTAACTCGAAGACAGTGAGTGAACGGATCACATTAAATTTACCAGCATC TACCCCAGTCCGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACATAAATGGAACCTTTGATTTG ACCAGGGAAAATGGAGTCACTACTGCTGACATGGCACCACTGGATCACACCAGTGACAAGTCTCTTCTTG ATGCTAATTTTGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACCGCCTCAGAT GTTGTTGGAGGACTCCAATAACGTGGATGACAGCGTGCATGACAGGTTCATAGGTCCACTGCCAAGAGAA GGTTCTGTGGCCTCTACCAATGATTATGTCAGCCAGAACTACTCCTATTCATCAATTTTGAACAAGTCAG AAACTGGTTATGTGGGATTAGTAAACCAGGCGATGACGTGCTATTTGAATAGCCTTTTGCAAACACTTTT TATGACTCCTGAATTTAGGAATGCATTATATAAGTGGGAATTTGAAGACTCTGAAGAAGATCCTGTAACG AGTATCCCATACCAGCTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAATTGAGACCA CAGATGTTACAAGGAGCTTTGGATGGGATAGCAGTGAGGCTTGGCAACAGCATGATGTACAAGAACTGTG CCGTGTTATGTTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTG TACCAAGGCAAGCTGAAAGATTATGTGAGGTGTCTGGAATGCGGTTATGAAGGCTGGAGAATTGACACGT ATCTTGATATTCCACTGGTCATCCGACCTTATGGGTCCAGCCAAGCGTTTGCTAGTGTGGAAGAAGCGTT GCATGCATTTATTCAACCAGAGATCTTGGATGGCCCAAATCAATATTTTTGTGAACGTTGTAAGAAGAAG TGTGATGCTCGGAAGGGCCTTCGGTTTTTGCATTTCCCCTATCTGCTGACCTTACAGTTGAAAAGGTTTG ATTTTGATTATACAACCATGCATAGAATTAAACTGAATGATCGAATGTCATTTCCGGAGGAGCTGGATAT GAGCACATTTATTGATATTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGATAGTGGAGCAGAA AATGAAGGCAGTTGCCACAGCGACCAGATGAGTAATGATTTCTCCACGGATGATGCTGTTGATGAAGGGA TATGCCTGGAGAGCAGTAGTGGGAGTGAGAAGATTTCAAAACCTGGCCTGGAAAAGAACTCCCTGATGTA TGAGCTCTTCTCAGTGATGGTGCATTCAGGGAGTGCTGCTGGAGGACACTATTATGCTTGCATAAAGTCT TTCAGTGACGATCAATGGTACAGTTTCAATGACCAACATGTGAGCAGGATAACACAAGAAGACATTAAGA AGACACATGGTGGGTCTTCAGGAAGCAGAGGCTACTACTCCAGTGCTTTTGCAAGCTCAACAAATGCATA TATGCTGATATACAGACTGAAGGATCCAACAAGAAATGCAAAGTTTCTAGAAGTGGACGAGTACCCAGAA CACATTAAGAATCTGGTGCAGAAGGAGAGAGAGCTGGAAGAGCAGGAAAAGAGGCAACGAGAGATTGAAC GCAACACGTGCAAGATAAAATTATTTTGTTTGCATCCTGTGAAACAAGTAATGATGGAAAACAAGCTGGA GGTTCATAAGGATAAGACGCTAAAGGAAGCCGTTGAAATGGCTTATAAGATGATGGATTTAGAAGATGTG ATTCCCTTGGATTGCTGTCGCCTGGTAAAATATGACGAGTTTCATGATTACCTGGAACGATCGTATGAGG GAGAAGAAGATACACCAATGGGGTTGCTTCTAGGAGGAGTCAAGTCCACATACATGTTTGATCTGCTGTT GGAAACGAGAAAGCCTGATCAAATTTTTCAGTCTTACAAACCTGGAGAAGTGATGGTGAAAGTTCATGTT GTTGATCTGAAAGCAGAGACTGTCGCTGCTCCTGTAACAGTGCGTGCGTATTTAAATCAGACAGTTACAG AGTTTAAACAGCTGATCTCAAAGGCCACGCACTTACCTGCAGACTCCATGAGGATAGTGCTGGAGCGCTG CTACAATGACCTGCGCCTTCTCAGCATGCCCAGCAAGACCCTGAAGGCTGAGGGCTTCTTCAGAAGTAAC AAGGTGTTTGTTGAAAGCTCTGAGACAGTGGATCACCAGGCAGCCTTTACAGACTCCCACTTGTGGAAAC TCCTGGATCGACATGCAAATACAATCCGGTTATTTGTTTTGCTGCCTGAACAATCTCCAGGATCATATTC GAAAAGGACAGCATACCAGAAAGCTGGAGGTGATTCTGGTAATGTGGACGATGATTGTGAAAGAGTCAAA GGACCTGCAGGGAATTTAAAGTCTGTGGATGCTATTCTAGAAGAGAGCACTGAAAAACTGAAAAGCTTGT CACTGCAGCAGCAGCAGCAAGATGGGGACAACGGAGACAGCAGCAAAAGCACTGAGACAAGTGACTTTGA AAATATTGAATCCCCTCTCAATGAGAGGGGCTCTTCCACCTCTGTGGATAATCGAGAACTTGAACAGCAC ATTCAGACTTCTGACCCAGAAAACTTTCAGTCTGAGGAACGCTCGGACTCAGATGTGAATAATGACCGGA GTACAAGTTCGGTAGACAGTGATATTCTTAGCTCCAGTCACAGCAGTGACACTTTGTGCAATGCAGACAG CGCTCAGATTCCTCTGGCCAATGGACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACTAAAGCAAAC GAGGGGAAAAAGGAGACCTGGGACACAGCTGAAGAAGACTCTGGGACTGACAGTGAATATGACGAGAGTG GCAAGAGCAGGGGGGACATGCAGTACATGTACTTCAAAGCTGACCCCTACACTGCGGATGAAGGCTCTGG GGAAGGACACAAATGGTTGATGGTGCATGTTGATAAGAGAATCACCCTGGCAGCTTTCAAACAGCATTTA GAACCTTTTGTTGGAGTCCTCTCCTCTCACTTCAAGGTTTTTCGAGTGTACACCAGCAATCAAGAGTTTG AGACCGTCCGGTTGAATGAGACCCTTTCATCATTTTCAGATGACAATAAGATCACAATCCGACTGGGGAG AGCACTGAAGAAGGGAGAGTACAGAGTTAAAGTGTGCCAGCTCTTAGTCAATGAGCAAGAGCCATGCAAG TTTCTGCTAGACGCTGTGTTTGCTAAAGGCATGACTGTGCGGCAATCAAAAGAGGAGCTGATTCCTCAAC TCAGGGAGCAGTGTGGCTTAGACCTCAGCATCGACAGGTTTCGTCTAAGGAAGAAAACATGGAAGAATCC TGGCACTGTCTTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCCAGCAACTGGGAAGTTTTC CTGGAAGTTCTTGATGGTGTGGAGAAGATGAAGTCCATGTCACAGCTTGCCATCTTAACAAGACGCTGGA GGCCTGCAGAGATGAAGTTGGACCCCTTCCAGGAGCTTGTGTTGGAAAGCAATAGTGTTGATGAACTCCG AGAGAAGCTAAGTGAAATCAGTGGGATTCCTTTGGAAGACATTGAATTTGCCAAGGGTAGGGGAACCTTC CCCTGTGATATTTCTGTCCTTGATATTCATCAAGATTTGGACTGGAATCCTAAAGTCTCTACCCTGAATG TTTGGCCTCTTTATATCTGTGATGATGGTGCCGTCATATTTTATAGGGATAGAACAGAAGAAGTGATGGA ATTGACAGATGAGCAAAGAAATGAATTGATGAAAAAGGAAAGCAGCCGACTGCAGAAGACGGGACATCGT GTGACATACTCCCCTCGCAAAGAGAAAGCACTCAAGATCTACCTGGATGGGGCGCCAAATAAAGATGTGG CTCAAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133758 |
| Insert Size | 4071 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133758.3, NP_598519.2 |
| RefSeq Size | 5540 bp |
| RefSeq ORF | 4071 bp |
| Locus ID | 74996 |
| UniProt ID | Q8BY87 |
| Gene Summary | Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER) (By similarity). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1, which results in a shorter isoform (2), compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226462 | Usp47 (tGFP-tagged) - Mouse ubiquitin specific peptidase 47 (Usp47), (10ug) |
CNY 11880.00 |
|
| MR226462 | Usp47 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 47 (Usp47), transcript variant 2 |
CNY 9280.00 |
|
| MR226462L3 | Lenti ORF clone of Usp47 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 47 (Usp47), transcript variant 2 |
CNY 12730.00 |
|
| MR226462L4 | Lenti ORF clone of Usp47 (mGFP-tagged) - Mouse ubiquitin specific peptidase 47 (Usp47), transcript variant 2 |
CNY 12730.00 |
