Hecw1 (NM_001081348) Mouse Untagged Clone
CAT＃: MC224744
Hecw1 (untagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), (10ug)
CNY 20620.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9330116H24Rik; AV273951; E130207I19Rik; Nedl1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224744 representing NM_001081348
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCACCTGTGTAGTGTGAAGAATCTGTACCAGAACAGGTTTTTAGGCCTGGCTGCCATGGCGT CTCCATCTAGAAACTCCCAGAGCCGACGCCGGTGCAAAGAACCCCTCCGGTACAGCTATAACCCAGACCA ATTCCACAACATAGACATCAGAAATGGTGCCCATGATGCTATCACCATCCCTCGCTCCACCAGTGACACT GACCTGGTCACTTCAGACAGCCGCTCTACACTCATGGTCAGCAGTTCCTACTATTCGATAGGGCACTCCC AGGATTTGGTGATCCACTGGGACATCAAGGAAGAAGTGGATGCTGGAGACTGGATTGGCATGTACCTTAT TGGTGAAGTCTCATCTGAAAACTTTCTGGATTATAAGAACCGTGGAGTCAATGGTTCTCACAGAGGCCAG ATCATCTGGAAGATTGATGCCAGCTCTTACTTTGTGGAATCTGAAACTAAGATCTGCTTCAAATACTACC ACGGAGTAAGTGGGGCCCTGCGAGCTACAACCCCCAGTGTCACTGTCAAGAACTCAGCAGCCCCTATTTT TAAAGGTATTGGCTCTGAAGAGACTGCACAAAGTCAAGGAAGCCGGAGGCTGATCAGCTTCTCCCTCTCA GATTTCCAGGCCATGGGATTGAAGAAAGGGATGTTTTTCAACCCCGACCCTTATCTGAAGATTTCCATTC AACCTGGGAAACATAGCATCTTCCCAGCCCTCCCTCATCATGGACAGGAAAGGAGATCCACGATCATAGG CAACACTGTAAACCCCATCTGGCAGGCAGAGCATTTCAGTTTCGTGTCTCTGCCAACCGATGTGCTGGAA ATTGAAGTGAAGGACAAGTTTGCCAAGAGTCGTCCCATCATCAAGCGTTTCTTGGGAAAGCTGTCAATGC CTGTTCAGAGGCTGCTGGAGAGACACGCCATAGGGGATAGGGTGGTCAGCTACACACTTGGCCGCCGGCT TCCAACTGACCATGTGAGTGGGCAGCTGCAATTCCGATTTGAGATCACATCCTCCATCCATGCAGATGAT GAAGAGATCTCTCTGAGTGCTGAGCCTGAGTCGTCAGCAGAAACGCAGGACAGCATCATGAATAGTATGG TGGGAAACAGCAATGGAGAGCCTTCTGGTGATGCTACAGAATTCTGCAAGGATGCGAAGCCAGAGTCACC AAGTGAAGGGAATGGAGTAAACAGCTCAGAAAACCAAAACCAGGAGCATGCCGGGCCAGTTGAGGAAGCG GCAGGAGCTATGGAGGCCAGAGATGGTAGCAATGTCTCTGAAGCACCCGAAGAGCCCGGAGAGCTTCAGG ACCCAGAGCAACATGACACCCAGCCCACCCTGAGTGCAGAAGAAGTGGCCGAGGGACTTCCCCTGGATGA GGATTCGCCATCCTCGCTGTTGCCAGAAGAGAATACAGCTCTAGGCAGCAAGGTAGAAGAGGAAACAGTA CCAGAAAATGGAGCCAGAGAGGAAGAAATGCAGAAGGGGAAAGATGAAGAAGAGGAAGAAGAGGACGTTT CTACCCTGGAGCAAGGAGAGCCTGGACTGGAGCTGAGGGTCTCCGTGAGAAAAAAAAGCAGACCATGTTC CCTACCTGTATCAGAGCTTGAGACAGTGATTGCATCTGCCTGTGGAGATGCTGAGACCCCGAGAACCCAT TATATCCGCATCCACACCCTGCTGCACAGCATGCCCTCGGCCCAGAGAGGAAGCACCACGGAGGAGGAAG ATGGCTTGGAGGAGGAATCCACCCTCAAGGAGTCCTCTGAAAAGGATGGGCTCAGCGAGGTGGACACAAT AGCTGCTGACCCACAGTCCATGGAAGATGGCGAGAGCGATGGGGCTACTCTGTGCATGGCACCCTCTGAC TGCTCTGGGGGACATTTCTCCAGTTTATCCAAAGGCATTGGTGCTGGCCAAGATGGCGAAGCTCACCCCA GCACTGGGAGTGAGAGTGACTCCAGCCCCCAGCAGGGAGCAGACCACAGTTGCGAGGGCTGTGATGCCTC ATGCTGCAGCCCGTCTTGCTATAGCACATCTTGCTACAGCAGCTCTTGTTACAGCAGCTCCTGTTACAGC AGCTCCTGCTACAATGGTAACAATCGGTTCGCCAGCCACACACGCTTCTCCTCTGTGGACAGCGCCAAGA TCTCTGAGAGCACAGTCTTTTCTTCACAAGAGGATGAGGAAGAGGAAAACAGTGCATTCGAGTCGGTACC CGACTCGGTGCAAAGCCCAGAGCTAGATCCCGAGTCTACAAATGGGGCTGGCCCTTGGCAGGATGAACTG GCTGCCCCTGGTGGGAATGCAGCAAGAAGTACTGAAGGTCTGGAGTCCCCCATGGCAGGTCCTAGCAATA GGAGAGAAGGTGAATGTCCTATACTCCATAATTCCCAGCCAATAAGCCAACTTCCTTCCTTGAGGCCTGA ACATCATCACTACCCAACAATCGATGAGCCTCTTCCACCAAACTGGGAGGCTCGAATTGACAGCCATGGA CGGGTCTTTTATGTGGACCATATAAATCGCACAACCACCTGGCAGCGACCATCGATGGCCCCTACCCCCG ATGGAATGATCAGATCCGGGTCTGTCCACCAGATGGAGCAGCTTAACAGAAGGTATCAAAACATCCAGAG GACCATGGCAACAGAGAGAGCTGAAGAAGATTCTGGCAATCAAAACTCTGAGCAAATACCAGACGGAGGA GGAGGAGGTGGTGGTGGGAGTGACTCGGAAGCAGAATCATCCCAATCAAGCTTAGATCTGAGGAGAGAAG GGTCACTTTCTCCTGTCAACTCACAGAAGGTCACCTTGCTGCTGCAGTCTCCAGCGGTCAAGTTCATCAC CAATCCTGAGTTCTTCACTGTGTTGCATGCCAATTATAGTGCCTACAGGGTTTTCACAAGCAGCACTTGC CTGAAGCATATGATTTTAAAAGTCCGAAGAGATGCTCGAAATTTTGAGCGCTATCAGCACAACCGGGACC TGGTGAATTTCATCAACATGTTTGCAGATACTCGGCTGGAACTGCCCCGGGGTTGGGAGATCAAAACTGA TCATCAGGGAAAGTCTTTCTTCGTGGACCACAACAGTCGAGCTACTACTTTCATAGATCCCCGAATCCCT CTTCAGAATGGCCGTCTTCCCAATCATCTAACCCACCGACAGCACCTCCAGAGGCTCCGGAGTTACAGTG CTGGGGAGGCCTCAGAAGTCTCTAGAAACAGAGGAGCATCTTTGCTGGCCAGACCAGGACACAGCCTAAT AGCTGCTATTCGAAGCCAACATCAACATGAATCCTTGCCACTGGCATACAATGACAAGATTGTGGCATTT CTTCGCCAGCCAAACATTTTTGAAATGCTACAAGAACGTCAGCCAAGCTTGGCAAGAAACCACACACTCA GGGAGAAAATCCATTATATTCGGACAGAGGGCAATCATGGGCTTGATAAATTGTCCTGTGATGCAGATCT AGTCATTTTGCTGAGTCTCTTTGAAGAAGAGATCATGTCCTATGTTCCCCTGCAATCTGCCTTCCACCCT GGATATAGCTTCTCTCCCCGCTGTTCACCCTGTTCCTCACCTCAGAACTCCCCAGGTTTACAGAGAGCCA GCGCAAGAGCCCCTTCACCATACCGAAGGGATTTTGAAGCTAAGCTCCGCAATTTCTACCGAAAACTGGA AGCCAAAGGATTTGGACAGGGTCCGGGTAAAATTAAGCTCATCATCCGTCGAGACCACTTGTTGGAGGGG ACCTTCAATCAAGTGATGGCATACTCAAGGAAGGAGCTCCAGAGAAACAAGCTCTACATCACCTTTGTTG GAGAGGAGGGGCTGGACTACAGTGGCCCTTCTCGAGAGTTCTTCTTCCTGTTGTCTCAGGAGCTCTTCAA CCCTTACTATGGACTCTTTGAGTATTCTGCAAATGACACATACACAGTACAGATCAGCCCCATGTCAGCA TTTGTAGAAAACTATCTTGAATGGTTTAGATTCAGTGGTCGTATCCTGGGCCTAGCTCTGATCCATCAGT ACCTCCTGGACGCTTTCTTCACAAGACCCTTCTATAAGGGACTCCTGAAGCTGCCATGTGATCTGAGTGA CCTAGAATATTTGGATGAGGAATTCCACCAGAGTTTACAGTGGATGAAGGACAACAACATCACAGACATT CTAGATCTCACCTTCACTGTTAATGAAGAAGTTTTTGGACAGGTAACAGAAAGAGAGTTGAAGTCTGGAG GCGCCAACACGCAGGTGACTGAGAAAAACAAGAAGGAATACATAGAGAGGATGGTGAAGTGGCGTGTGGA GCGTGGTGTGGTACAGCAGACGGAGGCATTGCTCAGGGGTTTCTATGAGGTTGTAGACTCAAGGCTCGTC TCAGTGTTTGATGCGAGGGAACTGGAGCTGGTGATCGCTGGAACTGCTGAAATCGACCTGAATGACTGGA GGAATAACACAGAGTACCGGGGAGGTTATCACGATGGCCACCTTGTGATCCGCTGGTTCTGGGCTGCAGT GGAACGCTTCAATAATGAGCAGAGATTGCGATTACTTCAGTTTGTGACAGGAACATCAAGTGTGCCCTAT GAAGGCTTTGCAGCTCTGCGGGGAAGCAATGGGCTTCGACGTTTCTGCATAGAAAAGTGGGGGAAGATTA CATCTCTTCCCAGGGCACATACGTGCTTCAACCGATTGGATCTCCCACCATACCCTTCATATTCCATGTT GTATGAAAAGCTGTTAACAGCAGTAGAAGAAACCAGCACCTTTGGACTTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001081348 |
| Insert Size | 4815 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001081348.3, NP_001074817.3 |
| RefSeq Size | 9460 bp |
| RefSeq ORF | 4815 bp |
| Locus ID | 94253 |
| UniProt ID | Q8K4P8 |
| Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218858 | Hecw1 (tGFP-tagged) - Mouse HECT C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), (10ug) |
CNY 13300.00 |
|
| MR218858 | Hecw1 (Myc-DDK-tagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1) |
CNY 10976.00 |
|
| MR218858L3 | Lenti ORF clone of Hecw1 (Myc-DDK-tagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1) |
CNY 13970.00 |
|
| MR218858L4 | Lenti ORF clone of Hecw1 (mGFP-tagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1) |
CNY 13970.00 |
