Scn3a (NM_018732) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gm1000; Nav1.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225075 representing NM_018732 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGGCACTGCTGGTACCCCCGGGACCTGAGAGCTTCCGCCTTTTCACTCGAGAATCTCTTGCTG CTATCGAAAAGCGTGCTGCAGAAGAGAAAGCCAAGAAACCCAAGAAAGAACAAGACATTGACGATGAGAA CAAGCCAAAGCCAAACAGTGACTTGGAAGCTGGGAAGAACCTTCCATTTATCTATGGAGACATTCCTCCA GAGATGGTGTCGGAGCCTCTGGAGGACCTGGACCCCTACTACGTCAGTAAGAAAACTTTTGTAGTGTTGA ATAAAGGGAAGGCAATTTTTCGATTCAGTGCCACCTCCGCCTTGTATATTTTAACTCCACTAAACCCCGT TAGGAAAATTGCTATTAAGATTTTGGTACACTCTTTATTCAGCATGCTTATCATGTGCACTATTTTGACC AACTGTGTATTTATGACATTGAGCAATCCTCCTGACTGGACGAAGAATGTAGAGTACACATTCACTGGGA TCTATACCTTTGAGTCACTTATAAAGATCTTGGCCAGAGGATTCTGCTTAGAAGATTTCACATTTCTTCG TGACCCATGGAACTGGCTGGATTTCAGTGTCATCGTGATGGCATATGTGACAGAGTTTGTGGACCTGGGC AATGTCTCAGCGCTGAGAACGTTCAGAGTTCTCCGAGCATTGAAAACAATATCAGTCATTCCAGGTTTAA AGACCATCGTGGGGGCCCTGATCCAGTCGGTGAAGAAGCTGTCTGACGTCATGATACTCACTGTGTTCTG TCTGAGCGTCTTTGCTCTCATCGGGCTGCAGCTCTTCATGGGCAACCTGAGGAATAAATGCTTGCAGTGG CCTCCAAGCGATTCTGCTTTTGAGATCAACACTACTTCCTACTTCAATGGCACAATGGACTCAAATGGGA CATTTGTTAATGTAACAATGAGCACATTCAACTGGAAGGACTATATCGCAGATGACAGCCACTTTTATGT TTTGGATGGACAAAAAGATCCTTTACTTTGTGGAAATGGGTCCGATGCAGGACAATGTCCAGAAGGGTAC ATCTGTGTGAAGGCTGGACGAAACCCCAACTACGGTTACACGAGCTTTGACACATTTAGCTGGGCCTTCT TATCGCTGTTTCGACTCATGACTCAAGACTACTGGGAGAACCTTTACCAGTTGACATTACGTGCAGCTGG GAAAACCTACATGATCTTTTTCGTCCTGGTAATTTTCTTGGGCTCATTTTATTTGGTGAACTTGATCCTG GCTGTGGTGGCCATGGCCTATGAGGAACAAAATCAGGCCACACTGGAGGAGGCTGAGCAGAAAGAGGCGG AGTTTCAGCAGATGTTGGAGCAGTTGAAAAAGCAGCAAGAGGAGGCTCAGGCGGTGGCAGCTGCCTCAGC AGCGTCCAGAGACTTCAGTGGGATAGGAGGGTTAGGAGAACTTCTGGAGAGTTCTTCAGAAGCTTCCAAG TTGAGCTCCAAGAGTGCCAAGGAGTGGAGGAATCGGAGGAAGAAGAGGAGACAGAGGGAGCACTTGGAGG GAAACCACAGACCCGAAGGAGACAGGTTTCCCAAGTCGGAATCAGAAGACAGCGTCAAGCGAAGGAGTTT CCTGTTCTCCCTGGATGGGAACCCGCTGAGCGGCGACAAGAAGCTGTGCTCTCCCCATCAGTCTCTCTTG AGTATCCGTGGCTCCCTGTTTTCCCCAAGACGCAATAGCAAAACGAGCATTTTCAGCTTCAGAGGTCGGG CGAAGGACGTGGGGTCTGAGAATGACTTTGCGGATGATGAACACAGCACCTTTGAAGATAGCGAGAGCAG GAGAGACTCACTGTTTGTGCCGCACAGACCTGGAGAGCGACGCAACAGTAACGGTACCACCACTGAAACA GAAGTCAGGAAGAGAAGGCTAAGTTCTTACCAGATCTCGATGGAAATGCTGGAGGATTCCTCTGGGAGAC AAAGAGCCATGAGCATAGCCAGTATCCTGACCAACACGATGGAGGAACTTGAAGAATCTAGACAGAAGTG TCCACCATGCTGGTATAGATTTGCCAATGTGTTTTTGATCTGGGACTGTTGTGATTCATGGTTGAAAGTA AAGCATCTTGTGAATTTGATTGTGATGGATCCATTTGTTGACCTGGCCATCACCATCTGCATCGTGTTAA ACACACTGTTCATGGCCATGGAGCACTACCCGATGACGGAGCAGTTCAGCAGTGTGCTGACGGTGGGAAA CCTGGTCTTCACCGGGATCTTCACAGCCGAGATGGTCCTGAAAATCATCGCAATGGATCCCTATTACTAT TTCCAAGAGGGCTGGAATATCTTTGATGGAATTATTGTTAGCCTGAGTTTAATGGAGCTTGGCCTGGCAA ACGTGGAGGGGCTGTCCGTGCTTCGGTCCTTCAGACTGCTGCGAGTCTTCAAGTTGGCAAAATCCTGGCC CACACTGAATATGCTCATTAAGATCATCGGCAACTCGGTGGGCGCACTGGGCAACCTGACCCTGGTGCTG GCCATCATCGTCTTCATTTTTGCCGTGGTCGGCATGCAGCTGTTTGGAAAGAGCTACAAGGAGTGTGTTT GCAAGATCAATGAGGACTGCAAGCTCCCGCGCTGGCACATGAACGACTTCTTCCACTCCTTCCTGATAGT GTTCCGCGTGCTGTGTGGGGAGTGGATAGAGACCATGTGGGACTGCATGGAGGTCGCGGGCCAGACCATG TGCCTTATTGTGTTTATGTTGGTCATGGTGATTGGGAACCTTGTGGTTCTGAACCTCTTCCTGGCCTTAT TGTTGAGTTCCTTTAGTTCAGACAACCTTGCTGCTACGGACGATGATAACGAAATGAACAACCTCCAGAT CGCGGTGGGAAGGATGCAAAAGGGGATTGATTATGTGAAAAATAAGATACGGGAGTGCTTCCGAAAAGCG TTTTTTAGAAAGCCGAAAGTGATAGAAATCCACGAAGGGAACAAAATAGACAGCTGCATGTCCAATAACA CGGGCGTAGTTGAAATAAGCAAAGAGCTTAACTACCTTAAAGACGGTAACGGAACCACCAGTGGCGTGGG TACTGGAAGCAGTGTGGAGAAATACGTAATTGATGAAAATGACTACATGTCATTCATCAACAACCCCAGC CTCACCGTGACGGTGCCAATTGCCGTGGGAGAGTCTGACTTTGAAAATTTAAACACGGAAGAGTTTAGCA GTGAGTCAGAACTGGAAGAAAGCAAGGAGAAATTAAATGCAACCAGCTCTTCTGAAGGAAGCACAGTTGA CGTTGCTCCGCCCCGAGAAGGTGAACAAGCTGAAATTGAACCTGAGGAGGACCTTAAGCCAGAAGCTTGC TTTACTGAAGGATGCATTAAAAAATTTCCCTTCTGCCAAGTAAGTACGGAAGAAGGTAAAGGAAAAATCT GGTGGAATCTTAGGAAGACCTGCTATAGCATTGTGGAACACAACTGGTTTGAGACGTTCATTGTGTTCAT GATTCTCCTCAGTAGTGGTGCTTTGGCCTTTGAAGATATATACATTGAGCAACGGAAGACCATCAAAACC ATGCTGGAGTATGCTGACAAAGTCTTCACTTACATCTTCATCCTGGAAATGCTCCTCAAATGGGTGGCCT ATGGATTTCAAACCTATTTCACCAATGCCTGGTGCTGGTTGGACTTCTTGATTGTTGATGTTTCTTTGGT TAGCCTGGTGGCCAACGCTCTTGGCTATTCAGAACTTGGTGCCATCAAATCCCTACGGACCCTGAGAGCT CTGAGGCCGCTCCGAGCCTTATCCCGCTTTGAAGGCATGAGGGTGGTTGTGAACGCTCTTGTTGGTGCAA TCCCCTCCATCATGAATGTGCTACTGGTGTGCCTCATCTTCTGGTTAATCTTTAGTATCATGGGTGTGAA TCTGTTTGCTGGAAAGTTCTATCACTGTGTTAACATGACAACGGGCAGCATGTTCGACATGAGTGAAGTC AACAATTTCAGCGACTGTCAGGCTCTTGGCAAGCAAGCCCGATGGAAGAATGTGAAAGTCAACTTTGACA ATGTTGGGGCTGGCTACCTGGCATTGCTGCAAGTGGCCACATTCAAAGGCTGGATGGATATTATGTATGC AGCTGTGGATTCACGGGACGTCAAACTGCAGCCTGTATATGAAGAAAATCTGTACATGTATCTGTACTTT GTCATCTTCATCATCTTTGGGTCGTTCTTCACTCTAAATCTATTCATCGGCGTCATCATAGACAACTTCA ACCAGCAGAAGAAGAAGTTTGGAGGTCAAGACATCTTTATGACAGAAGAGCAGAAAAAGTACTACAATGC AATGAAGAAACTTGGCTCCAAAAAACCTCAGAAGCCCATCCCTCGACCTGCAAACAAATTTCAAGGAATG GTCTTTGACTTTGTAACCAGACAAGTGTTTGACATCAGCATCATGATCCTCATCTGCCTCAACATGGTGA CCATGATGGTGGAAACGGACGACCAGAGCAAATACATGACCCTGGTTTTGTCCCGAATCAACCTGGTATT CATCGTCCTCTTCACTGGGGAGTTTCTGCTGAAGCTCATCTCTCTCAGATACTACTACTTCACGATTGGC TGGAACATCTTTGACTTTGTGGTGGTGATTCTCTCAATTGTAGGAATGTTCCTTGCTGAGCTGATAGAGA AGTATTTTGTGTCTCCTACCCTGTTCCGAGTCATCCGCCTGGCCAGGATTGGACGAATCCTACGCCTGAT CAAAGGCGCCAAGGGGATCCGCACGCTGCTCTTTGCTCTGATGATGTCCCTTCCTGCGCTGTTCAACATC GGCCTCCTGCTTTTCCTCGTCATGTTCATCTACGCCATCTTTGGGATGTCCAACTTTGCCTATGTTAAAA AAGAGGCTGGAATTGATGACATGTTCAACTTTGAGACTTTTGGCAACAGCATGATCTGCCTGTTCCAAAT CACCACCTCTGCGGGCTGGGATGGACTGTTGGCCCCCATCCTCAACAGTGCACCTCCTGACTGTGACCCT GATGCAATTCACCCTGGAAGCTCAGTGAAGGGAGACTGTGGGAACCCATCTGTGGGGATTTTCTTTTTTG TCAGCTACATCATCATATCCTTCCTGGTTGTGGTGAACATGTACATTGCTGTCATCCTGGAGAACTTCAG CGTTGCCACAGAAGAAAGTGCAGAGCCCCTGAGTGAGGACGACTTTGAGATGTTCTACGAGGTCTGGGAG AAGTTCGACCCTGACGCCACCCAGTTCATAGAGTTCTGCAAGCTCTCTGACTTTGCAGCTGCCCTGGATC CTCCCCTCCTCATCGCAAAGCCAAACAAAGTCCAGCTCATTGCCATGGACCTGCCCATGGTGAGTGGAGA CCGCATCCACTGCCTGGACATCTTATTTGCTTTTACAAAGCGGGTCCTGGGTGAGAGTGGAGAGATGGAT GCCCTTCGAATCCAGATGGAAGATCGGTTCATGGCTTCCAATCCCTCCAAGGTCTCTTATGAGCCCATTA CCACCACTCTGAAGCGCAAACAAGAGGAGGTGTCTGCTGCTATCATTCAGCGTAATTACAGATGTTATCT TTTAAAGCAAAGGTTAAAAAACATATCAAATACGTATGACAAAGAGACAATCAAGGGGAGGATTGTCTTG CCTATAAAAGGAGATATGGTTATTGACAAATTAAATGGGAATTCCACCCCAGAAAAGACAGATGGGAGTT CCTCTACCACCTCTCCTCCTTCCTATGACAGTGTAACAAAACCAGATAAGGAAAAGTTTGAGAAAGACAA ACCAGAAAAAGAAAGCAAAGGGAAAGAGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_018732
Insert Size	5844 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_018732.3, NP_061202.3
RefSeq Size	9560 bp
RefSeq ORF	5844 bp
Locus ID	20269
UniProt ID	A2ASI5
Gene Summary	Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:29142310). May contribute to the regulation of serotonin/5-hydroxytryptamine release by enterochromaffin cells (PubMed:29142310). In pancreatic endocrine cells, required for both glucagon and glucose-induced insulin secretion (PubMed:25172946).[UniProtKB/Swiss-Prot Function]

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