Dhx34 (NM_001285931) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200013B07Rik; 1810012L18Rik; Ddx34; mKIAA0134
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229410 representing NM_001285931 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCTCCCAGGACAAGGGAGGGGAGGGGTCACCGAGACCGAGACCACCATCGAGCTCCCAGGGAGG AAGAGGCCCCGGAGAAATGGGACTGGAATTGTCCAGAGACTCGTTGTCTCTTGGAAGATGTATTCTTCCG CGATGAGGATTATATCCGTCGTGGTTCAGAGGAGTGTCAGAAGTTCTGGGCTTTCTTTGAACGCCTGCAG AGATTCCAGCACCTCAAGACCTCCCAGAAGAAGGAGAAGGACCCTGGCATGCCCAAACATGGCATCGCTG CTTTAGCTGACCTACCTCTCACTTACGACCCACGCTACCGCATCAACCTCTCCATTCTCAGCCCAGACAC TCGGGGTCGTCATGGGCCCGGCAGGGGGCTGCCGCCCGAGAGAGTGTCCGAGTTCCGCCGTGCATTGCTT CACTACCTAGACTTCCAGCAGAAGCAGGCTTTTGGGAGGCTCGTCAAACTGCAGCGTGAGCGAGCGGCAC TTCCCATCGCCCAGTATGGGAACCGCATCCTGCAGACTCTCAAGGAACACCAGGTGGTGGTGGTGGCCGG GGACACAGGCTGTGGCAAGTCTACTCAGGTGCCCCAGTACTTGCTGGCCGCCGGCTTCAGTCACGTGGCA TGCACTCAGCCCCGGAGAATCGCCTGCATCTCACTGGCCAAGCGCGTTGGCTTCGAGAGCCTCAGTCAGT ATGGCTCCCAGGTTGGCTACCAGATCCGCTTTGAAAGCACTCGGTCGGCAGCCACCAAGATCGTGTTCCT GACGGTGGGGCTGCTCCTACGCCAGATCCAGCGTGAGCCCAGCCTGCCCCAGTACCAGGTCCTGATCGTG GACGAGGTCCACGAGCGACACCTGCACAATGACTTCCTGCTGGGTGTCCTGCAGCGCCTGCTGCCCCAGC GGCCTGACCTCAAGGTCATCCTCATGTCGGCCACCATCAACATCTCTCTCTTCTCCAGCTACTTCAGCCA TGCTCCGGTGGTGCAGGTGCCTGGGAGGCTCTTCCCCATCACGGTAGTCTACCAGCCGCAGGAGGCAGAC CAGACAGCATCCAAGTCAGAGAAGCTGGACCCACGGCCCTTCCTGAGGGTGCTGGAGGCCATCGACAATA AGTATCCCCCCGAGGAGCGGGGGGACCTTCTTGTCTTCCTCAGTGGCATGGCGGAGATCACCACGGTGCT GGACGCAGCCCAGGCTTATGCCAGCCTCACCCAGCGCTGGGTGGTGCTGCCGCTGCACAGCGCCCTCTCT GTGTCTGACCAGGACAAGGTGTTCGATGTGGCTCCAGCTGGAGTCCGGAAGTGCATCCTGTCCACCAACA TTGCCGAGACCTCAGTCACGATCGATGGCATCCGCTTCGTGGTGGATTCTGGGAAGGTGAAGGAGATGAG CTACGACCCACAGGCCAAACTGCAGCGGCTGCAGGAATTCTGGATCAGCCAGGCCAGTGCTGAGCAGCGC AAGGGCCGAGCGGGCCGCACGGGCCCCGGCGTCTGCTACCGTCTCTATGCCGAGTCTGACTATGATGCCT TTGCGCCGTACCCAGTCCCGGAAATCCGCAGGGTGGCCCTGGATGCACTGGTGCTTCAGATGAAGAGTAT GAGTGTGGGGGACCCACGCACCTTCCCCTTCATCGAGCCCCCTCCACCTGCCAGCGTGGAAACCGCTATC CTCTATCTCCAAGAGCAGGGGGCCCTGGACAGCTCGGAGGCCCTCACCCCCATCGGGTCCCTGCTGGCCC AGCTGCCTGTAGATGTTGTAATTGGGAAGATGCTGATCCTCGGCTCCATGTTCAGCCTGGCAGAGCCTGT GCTCACCATTGCTGCTGCCCTCAGCGTCCAGTCACCATTCACCCGCAGTGCCCAGAGCAACCTGGACTGT GCCACTGCCCGGAGACCGCTGGAAAGTGACCAGGGTGACCCCTTCACACTCTTCAATGTCTTCAATGCCT GGGTGCAGGTGAAGTCTGAGCGGAGCGGAAACTCTCGAAAGTGGTGCCGCCGCCGGGGTGTGGAGGAGCA CCGGCTGTATGAGATGGCCAACCTGCGGCGCCAGTTCAAGGAGCTGTTGGAGGATCATGGATTGCTGAGT GGGGCCCAGGTAGTGGCCCCAGGGGACAGCTACAGTCGGCTGCAGCAGCGCCGAGAGCGCAGGGCCCTGC ACCAGCTGAAGCGACAGCATGAGGAGGGTGGGGGACGCAGGCGCAAGGTGCTGAGGCTGCAGGAGGATGG CTGCTCCAGCGATGAAGAGGACCGAGAGGGCTCTACCTCCCAGAGGGCTGACAGCGTTGACATCCAGGAT GTGAAATTCAAGCTTCGGCACAACCTGGAGCAGCTGCAGGCAGCTGCCAGCTCAGCCCAGGACCTGACCC GAGACCAACTGGCCCTGCTCAAGCTGGTGCTCGGGCGGGGGCTGTACCCACAGCTGGCTGTGCCCGATGC GTTCAACAGTGGCCGCAAGGACTCAGACCAGATTTTCCACACGCAGGCCAAGCAGGGCACCGTGCTGCAC CCCACCTGTGTCTTTGCCAACAGCCCTGAGGTATTACACACACAGGGGCAGGAGGCCTCAGGCCAAGAAG GGAGCCAAGATGGCAGGGACCAAATGAGCTGCAAGCATCAGCTGCTGGCCTTCGTCTCCCTACTGGAGAC CAACAAGCCATACCTGGTGAACTGCGTCAGAATCCCGGCCCTCCAGTCCCTCCTGCTGTTCAGCCGCTCC ATTGACACCAACGGTGACTGCTCCCGCCTGGTGGCTGACGGTTGGCTAGAGCTGCAGCTCGCAGATAGCG AGAGTGCCGTCCGGCTCCTGGCAACATCCCTGCGGCTCCGTGTCCACTGGGAAAGTGCCCTGGACCGGCA GCTGGCCCGCCAGGCCCAGCGACGGAAGCTAGAGCAGGAGGAAGACATGGGGTCTCCAGCAGTCAGCCCC CAGGAGGTGGCTGCTCTGAGCAGGGAGCTGCTGCAGTTCATGGTGGCCAAGGTTCCCTACCGCCTCCGAA GGCTCACTGGTCTAGAAGCCCAGAACCTCTACGTGGGACCCCAGACCATCACGACCGCCCCTAGTCTGCC CGGCCTCTTTGGAAATTCTACCCTGTCTCCACACCCCACCAAGGGGGGCTACGCTGTCTCTGATTATCTC ACCTACAACTGCCTCACGAGTGACACTGACCTGTACAGTGACTGTCTGCGTTCCTTCTGGACCTGCCCAC ACTGTGGCCTGCACATGCCCTTCACACCCTTGGAGCGCATTGCTCATGAGAACACCTGCCCTGAGGCGCC AGGCGATGATCCAGGGTCTGAGGAGGCTGCTCCTGCACCACCACAGAAGACATCAGCCCTGCAGAGGCCC TACCACTGCCAGGTGTGCGGGCAGGACTTCCTCTTTACCCCAACTGAGGTACTAAGACATCGAAGGCAGC ATGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285931
Insert Size	3438 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285931.1, NP_001272860.1
RefSeq Size	4310 bp
RefSeq ORF	3438 bp
Locus ID	71723
UniProt ID	Q9DBV3
Gene Summary	Probable ATP-binding RNA helicase.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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