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Rap2b (NM_028712) Mouse Tagged ORF Clone
CAT＃: MG201667
- TrueORF®
Rap2b (tGFP-tagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b)
ORF Plasmid: DDK
"NM_028712" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4021402C18Rik; AA408554 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG201667 representing NM_028712
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGAGTACAAAGTGGTGGTACTGGGCTCGGGCGGCGTGGGCAAGTCCGCGCTCACCGTGCAGTTCG TAACAGGTTCCTTCATCGAGAAGTACGACCCGACCATCGAGGACTTTTACCGCAAGGAGATCGAGGTGGA CTCGTCGCCGTCGGTGCTGGAGATCTTGGACACCGCGGGCACGGAGCAGTTCGCCTCAATGCGGGACCTG TACATCAAGAATGGCCAGGGCTTCATTCTCGTCTACAGCCTGGTCAACCAGCAGAGCTTCCAGGACATCA AGCCCATGCGGGACCAGATCATCCGCGTGAAGCGGTACGAGCGCGTACCCATGATCCTGGTAGGCAACAA GGTGGACTTGGAGGGTGAACGTGAGGTCTCCTATGGCGAGGGTAAGGCCCTGGCCGAGGAGTGGAGCTGC CCCTTCATGGAGACATCGGCCAAAAACAAAGCCTCAGTGGATGAGCTATTCGCAGAGATCGTGAGGCAGA TGAACTACGCGGCACAGCCCAACGGCGACGAGGGCTGCTGCTCGGCCTGCGTGATCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201667 representing NM_028712
Red=Cloning site Green=Tags(s) MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDL YIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVSYGEGKALAEEWSC PFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCCSACVIL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028712 |
| ORF Size | 549 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028712.4, NP_082988.1 |
| RefSeq Size | 4197 bp |
| RefSeq ORF | 552 bp |
| Locus ID | 74012 |
| UniProt ID | P61226 |
| Gene Summary | Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204890 | Rap2b (untagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b), (10ug) |
CNY 2,000.00 |
|
| MR201667 | Rap2b (Myc-DDK-tagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b) |
CNY 2,400.00 |
|
| MR201667L3 | Lenti ORF clone of Rap2b (Myc-DDK-tagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b) |
CNY 4,750.00 |
|
| MR201667L4 | Lenti ORF clone of Rap2b (mGFP-tagged) - Mouse RAP2B, member of RAS oncogene family (Rap2b) |
CNY 4,750.00 |
