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Fam20c (NM_030565) Mouse Tagged ORF Clone
CAT＃: MG203162
- TrueORF®
Fam20c (tGFP-tagged) - Mouse cDNA sequence BC004044 (BC004044)
ORF Plasmid: DDK
"NM_030565" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C76981; DMP-4; DMP4; GEF-CK; mKIAA4081 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203162 representing NM_030565
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAACATGACCAAGGAGATCCGGGATGTGACGCGGGATAAGAAGCTATGGAGAACTTTCTTTGTGT CTCCAGCCAACAACATCTGTTTCTACGGGGAGTGTTCCTACTACTGTTCCACCGAGCATGCCCTGTGTGG GAGGCCCGACCAGATCGAAGGATCCCTGGCGGCCTTCCTGCCTGACCTGTCGCTGGCCAAGAGGAAGACA TGGCGGAACCCCTGGCGTCGTTCCTACCACAAGCGAAAGAAGGCAGAGTGGGAAGTCGACCCTGATTACT GTGAGGAGGTGAAGCAGACGCCACCCTACGACAGTGGCCACCGAATCCTGGACATCATGGATATGACCGT CTTTGATTTCCTCATGGGGAACATGGATCGGCATCACTACGAGACCTTTGAGAAGTTCGGGAATGAGACC TTCATCATCCACTTGGACAACGGGCGCGGGTTTGGGAAATACTCACACGATGAGCTTTCCATTCTCGCTC CTCTTCATCAGTGCTGCAGGATCAGGAGGTCCACCTACCTGAGACTGCAGCTGCTGGCCAAAGAGGAACA CAAACTGAGCCTGCTGATGGCCGAGTCCCTGCAGCATGACAAGGTGGCACCCGTGCTCTACCAGCTGCAT CTGGAGGCCCTGGACCGGCGGTTGCGCATAGTGCTGCAGGCTGTGCGAGACTGTGTGGAGAAGGACGGGC TGAGCAGTGTGGTGGAGGACGACCTAGCCACTGAGCACAGAGCCTCCACGGAGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203162 representing NM_030565
Red=Cloning site Green=Tags(s) MINMTKEIRDVTRDKKLWRTFFVSPANNICFYGECSYYCSTEHALCGRPDQIEGSLAAFLPDLSLAKRKT WRNPWRRSYHKRKKAEWEVDPDYCEEVKQTPPYDSGHRILDIMDMTVFDFLMGNMDRHHYETFEKFGNET FIIHLDNGRGFGKYSHDELSILAPLHQCCRIRRSTYLRLQLLAKEEHKLSLLMAESLQHDKVAPVLYQLH LEALDRRLRIVLQAVRDCVEKDGLSSVVEDDLATEHRASTER TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030565 |
| ORF Size | 747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030565.5, NP_085042.1 |
| RefSeq Size | 3009 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 80752 |
| UniProt ID | Q5MJS3 |
| Gene Summary | Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth (PubMed:22900076, PubMed:22732358, PubMed:25789606). Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome (By similarity). Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity (By similarity). Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1 (PubMed:25789606). In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201959 | Fam20c (untagged) - Mouse family with sequence similarity 20, member C (Fam20c), (10ug) |
CNY 2,000.00 |
|
| MR203162 | Fam20c (Myc-DDK-tagged) - Mouse family with sequence similarity 20, member C (Fam20c) |
CNY 2,400.00 |
|
| MR203162L3 | Lenti ORF clone of Fam20c (Myc-DDK-tagged) - Mouse family with sequence similarity 20, member C (Fam20c) |
CNY 4,750.00 |
|
| MR203162L4 | Lenti ORF clone of Fam20c (mGFP-tagged) - Mouse family with sequence similarity 20, member C (Fam20c) |
CNY 4,800.00 |
