Mrg2 (BC003762) Mouse Tagged ORF Clone

CAT#: MG205536

  • TrueORF®

Mrg2 (tGFP-tagged) - Mouse myeloid ecotropic viral integration site-related gene 2 (cDNA clone MGC:5914 IMAGE:3593200)

ORF Plasmid: DDK tGFP



  "BC003762" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2280.00


货期*
4周

规格
    • 10 ug

Product images

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Meis3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG205536 representing BC003762
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGGAGGTATGATGAGCTGCGCCACTACCCAGGCATTACAGAGCACATGACAGCCCTGGCCAGCT
TTTCAGAGGCAGCGCCTTCAGTACCTAGAGCCCCTGGGCCCTACACCCCACACCGGCCACCCCAGCTCCA
AGCTCCAGGCTTGGACAGCGACAGCTTGAAGAGAGAGAAGGATGACATCTATGGACACCCCCTCTTCCCA
CTCTTGGCCCTGGTCTTTGAGAAGTGTGAGCTGGCTACATGCTCGCCCCGTGATGGGGCCTCGGCTGGGC
TGGGTTCACCCCCAGGTGGTGACGTCTGCTCCTCTGACTCCTTCAATGAGGATATTGCTGCCTTTGCTAA
GCAGATCCGCTCCGAGAGGCCGCTGTTCTCTTCCAACCCGGAGCTGGACAACCTGATGGTCCAGGCCATC
CAGGTACTCCGGTTCCACCTGCTGGAGCTGGAGAAGGGGAAGATGCCCATAGACCTGGTGATTGAGGATC
GGGATGGTAGTTGCAGGGAGGATCTTGAGGACTACGCAGCCTCCTGCCCCAGCCTCCCAGACCAGAATAC
TACATGGATCAGAGACCATGAGGACAGTGGGTCTGTACATCTGGGGACCCCAGGTCCATCCAGCGGAGGC
CTGGCCTCCCAGAGTGGGGACAACTCAAGTGACCAAGGAGATGGGCTGGACACAAGCGTAGCCTCTCCCA
GCTCCGCAGGAGAGGATGAGGACCTGGACCTGGAACGCAGACGGAACAAGAAGAGGGGGATCTTTCCCAA
AGTGGCCACCAACATCATGAGGGCCTGGTTGTTCCAGCATCTCTCGCACCCGTACCCTTCAGAAGAGCAA
AAGAAACAGCTGGCCCAGGACACAGGGCTCACCATCCTGCAGGTGAACAACTGGTTTATTAATGCCCGGA
GACGAATAGTGCAGCCCATGATTGACCAGTCTAATCGCACAGGGCAGGGTGCATCTTTCAACCCTGAGGG
CCAGCCCATGGCAGGCTTCACAGAGACTCAGCCACAAGTGACAGTCAGGACACCAGGATCAATGGGGATG
AACCTGAACTTAGAAGGAGAGTGGCATTACCTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG205536 representing BC003762
Red=Cloning site Green=Tags(s)

MARRYDELRHYPGITEHMTALASFSEAAPSVPRAPGPYTPHRPPQLQAPGLDSDSLKREKDDIYGHPLFP
LLALVFEKCELATCSPRDGASAGLGSPPGGDVCSSDSFNEDIAAFAKQIRSERPLFSSNPELDNLMVQAI
QVLRFHLLELEKGKMPIDLVIEDRDGSCREDLEDYAASCPSLPDQNTTWIRDHEDSGSVHLGTPGPSSGG
LASQSGDNSSDQGDGLDTSVASPSSAGEDEDLDLERRRNKKRGIFPKVATNIMRAWLFQHLSHPYPSEEQ
KKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRTGQGASFNPEGQPMAGFTETQPQVTVRTPGSMGM
NLNLEGEWHYL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN BC003762
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC003762, AAH03762
RefSeq Size 1721 bp
RefSeq ORF 1085 bp
Locus ID 17537
Gene Summary The protein encoding this gene belongs to the three amino acid loop extension family of homeodomain transcription factors, which play essential roles in many embryonic processes. These proteins are characterized by an atypical homeodomain containing a three amino acid loop extension between helices 1 and 2. Expression of this gene begins during the compaction stage of embryogenesis and continues into the blastocyst stage. This gene is also expressed in pancreatic islet cells and beta-cells and regulates beta-cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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